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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3651 and PP_3652 are separated by 22 nucleotides
PP_3652 and PP_3653 are separated by 66 nucleotides
PP_3653 and PP_3654 are separated by 111 nucleotides
PP_3654 and PP_3655 are separated by 396 nucleotides
PP_3651: PP_3651 - Sensor histidine kinase/response regulator, at 4,145,918 to 4,148,101
_3651
PP_3652: PP_3652 - Transporter, LysE family, at 4,148,124 to 4,148,756
_3652
PP_3653: PP_3653 - Transporter, LysE family, at 4,148,823 to 4,149,437
_3653
PP_3654: PP_3654 - leucine-responsive regulatory protein, at 4,149,549 to 4,150,001
_3654
PP_3655: PP_3655 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,150,398 to 4,151,810
_3655
Group
Condition
PP
_3651
PP
_3652
PP
_3653
PP
_3654
PP
_3655
nitrogen source
D-2-Aminobutyric (N)
-0.4
-1.0
-4.9
-5.7
-0.1
nitrogen source
D-2-Aminobutyric (N)
-0.2
-0.7
-4.3
-5.0
+0.4
nitrogen source
DL-2-Aminobutyric (N)
+0.1
-0.1
-2.8
-4.2
+0.1
nitrogen source
DL-2-Aminobutyric (N)
+0.0
-0.3
-2.1
-3.8
+0.2
temperature shift
Temperature shift 30_to_10; with MOPS
-0.6
-0.2
-0.7
-0.8
-0.1
carbon source
D-Glucose (C)
+0.1
-0.0
-1.0
-0.7
-0.1
carbon source
L-Leucine (C)
+0.0
-0.1
-0.3
-1.3
-0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
-0.2
-0.9
-0.9
+0.3
temperature shift
Temperature shift 30_to_10; with MOPS
-0.6
-0.1
-0.2
-0.6
+0.0
temperature shift
Temperature shift 30_to_4
-0.8
-0.4
-0.1
-0.5
+0.2
pH
Growth at pH6 and (C) D-Glucose
-0.2
-0.3
+0.1
-1.1
-0.0
carbon source
Trisodium citrate (C)
+0.2
-0.6
-0.1
-0.8
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.0
+0.2
-0.4
-1.0
-0.4
temperature
Growth at 25C
-0.1
-0.1
+0.1
-0.9
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.1
+0.2
-0.3
-0.6
-0.3
pH
Growth at pH9 and (C) Trisodium citrate
+0.2
-0.6
-0.8
+0.2
-0.2
pH
Growth at pH9 and (C) Trisodium citrate
+0.1
-0.5
-0.5
+0.3
-0.3
carbon source
carbon source 2-Deoxy-D-glucose 10 mM
-0.3
-0.4
+0.4
-0.4
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.3
-0.4
+0.6
-0.5
-0.0
carbon source
ferulate (C); with MOPS
+0.8
-0.6
-0.4
-0.3
-0.2
carbon source
ferulate (C); with MOPS
+0.4
-0.4
-0.5
-0.2
+0.1
pH
Growth at pH7 and (C) Trisodium citrate
+0.4
-0.6
-0.5
+0.4
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.2
+0.3
+0.3
-0.7
-0.2
phage
P. putida Missley MOI 0.1
-0.2
-0.7
+0.2
+0.4
+0.1
stress
Growth with Chloride 500 mM; with MOPS
+0.2
-0.3
-0.9
+0.5
+0.3
phage
P. putida Sci small MOI 0.1
-0.0
-0.3
+0.6
-0.7
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.0
-0.4
-0.3
+0.8
-0.1
carbon source
Valerolactone (C)
+0.9
-0.2
-0.2
+0.4
+0.2
nitrogen source
L-Methionine (N)
-0.1
-0.0
+5.8
+5.7
+0.0
nitrogen source
L-Methionine (N)
-0.2
+0.0
+6.2
+6.1
+0.0
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