Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3577 and PP_3578 are separated by 78 nucleotidesPP_3578 and PP_3579 are separated by 173 nucleotidesPP_3579 and PP_3580 are separated by 229 nucleotidesPP_3580 and PP_3581 overlap by 4 nucleotides PP_3577: PP_3577 - RNA polymerase, sigma 19 factor, at 4,058,670 to 4,059,182 _3577 PP_3578: PP_3578 - phosphoglucomutase, at 4,059,261 to 4,060,898 _3578 PP_3579: PP_3579 - conserved membrane protein of unknown function, at 4,061,072 to 4,061,719 _3579 PP_3580: PP_3580 - conserved protein of unknown function, at 4,061,949 to 4,062,254 _3580 PP_3581: PP_3581 - conserved membrane protein of unknown function, at 4,062,251 to 4,064,338 _3581
Group Condition PP_3577 PP_3578 PP_3579 PP_3580 PP_3581
phage P. putida Sci small MOI 0.1 +0.3 -0.2 -0.5 -5.2 -0.1
pH Growth at pH6 and (C) D-Glucose -0.0 -0.1 +0.4 -5.8 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.1 -0.4 -3.4 +0.0
carbon source acetate (C) -0.2 -0.4 -0.9 -2.3 +0.1
nitrogen source nitrate (N) +0.1 -0.3 +0.1 -3.6 -0.0
pH Growth at pH9 and (C) Trisodium citrate -0.2 -0.2 +0.1 -3.3 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.1 +0.1 -0.4 -2.9 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 -0.1 -1.0 -2.2 -0.1
carbon source Tween 20 (C) -0.9 +0.1 -0.4 -1.3 -0.1
phage moi 100 Time6-phageFRS +0.0 -0.2 +0.1 -2.6 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 +0.4 +0.4 -3.6 +0.4
carbon source D-Glucose (C); with MOPS +0.1 -0.2 +0.1 -2.4 -0.0
phage P. putida PUT10 MOI 0.1 -0.2 +0.0 -0.0 -2.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.0 +0.0 -0.4 -2.2 +0.1
temperature shift Temperature shift 30_to_25 -0.2 -0.2 -0.1 -1.9 +0.1
stress Growth with Chloride 500 mM; with MOPS -0.5 -0.3 -0.2 -1.6 +0.3
pH Growth at pH6 and (C) Trisodium citrate -0.1 -0.0 +0.3 -2.3 -0.2
no stress control Growth with no stress -0.3 -0.4 -0.5 -1.1 +0.1
nitrogen source Propandiamine (N) +0.2 +0.2 -0.6 -1.5 -0.3
nitrogen source Ammonium chloride (N) +0.4 -0.4 +0.0 -2.0 +0.1
pH Growth at pH8 and (C) Trisodium citrate -0.0 -0.2 +0.3 -2.1 +0.3
carbon source 3-Hydroxydecanoic (C) +0.2 +0.1 -0.3 -1.8 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.1 +0.1 -0.0 -2.0 +0.2
phage P. putida Sci Big MOI 0.1 -0.1 +0.0 +0.4 -2.2 +0.4
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.3 -0.3 -0.3 -1.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.4 +0.2 -2.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 +0.6 -0.5 -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 -0.3 +0.7 -1.5 +0.1
temperature shift Temperature shift 30_to_25 +0.3 -0.5 +0.0 +1.5 +0.2
phage PUT 8 MOI 1 +0.4 +0.1 -0.1 +1.9 +0.1
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