Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3561 and PP_3562 overlap by 8 nucleotidesPP_3562 and PP_3563 are separated by 206 nucleotidesPP_3563 and PP_3564 are separated by 1 nucleotidesPP_3564 and PP_3565 are separated by 104 nucleotides PP_3561: PP_3561 - conserved membrane protein of unknown function, at 4,040,113 to 4,041,060 _3561 PP_3562: PP_3562 - conserved exported protein of unknown function, at 4,041,053 to 4,041,706 _3562 PP_3563: PP_3563 - conserved exported protein of unknown function, at 4,041,913 to 4,042,764 _3563 PP_3564: PP_3564 - Transcriptional regulator, AraC family, at 4,042,766 to 4,043,743 _3564 PP_3565: PP_3565 - Transporter, LysE family, at 4,043,848 to 4,044,459 _3565
Group Condition PP_3561 PP_3562 PP_3563 PP_3564 PP_3565
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 -0.6 +0.1 -1.8 +0.0
phage P. putida Sci Big MOI 0.1 -0.1 +0.4 -1.4 -1.0 -0.0
nitrogen source nitrate (N) +0.0 +0.2 -0.2 -1.2 -0.8
carbon source ferulate (C); with MOPS +0.0 -0.5 -1.4 -0.2 +0.3
r2a control with 0.2% methanol R2A control with 0.2% methanol -0.1 -0.4 -1.4 +0.3 -0.1
pH Growth at pH6 and (C) Trisodium citrate +0.0 -0.5 -0.8 +0.1 -0.6
carbon source D-Glucose (C); with MOPS -0.4 -0.5 -0.8 -0.0 +0.2
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.2 -0.2 -1.0 -0.4 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.5 -0.6 -0.5 +0.3
pH Growth at pH6 and (C) D-Glucose -0.3 +0.4 -0.2 -1.0 -0.2
phage P. putida Missley MOI 0.1 +0.4 -0.4 -0.5 -0.1 -0.6
nitrogen source D-2-Aminobutyric (N) -0.1 +0.5 -0.4 -0.8 -0.2
stress Growth with Chloride 500 mM; with MOPS +0.3 -0.3 -1.2 +0.2 -0.1
carbon source acetate (C) -0.1 -0.5 +0.5 -0.7 -0.2
phage P. putida PUT 7 MOI 1 -0.2 +0.2 -0.6 +0.4 -0.6
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 -0.1 -0.8 -0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.0 -1.4 +0.4 -0.0
carbon source Tween 20 (C) +0.3 +0.1 -1.1 +0.1 -0.2
nitrogen source nitrite (N) +0.4 +0.2 -1.2 +0.2 +0.0
nitrogen source D-2-Aminobutyric (N) +0.1 +1.0 -0.7 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.2 -0.8 +0.3 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.5 -1.2 -0.3 +0.7
pH pH 5 -0.0 -0.4 -0.4 +0.4 +0.6
nitrogen source nitrogen source nitrite 0.5mM +0.2 +0.0 +1.1 -0.7 -0.2
carbon source D-Glucose (C) -0.5 +0.2 +0.7 -0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.0 -0.6 +1.3 -0.1
carbon source Valerolactone (C) +0.4 -0.6 +0.3 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.5 +1.5 -0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +1.4 -0.4 -0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.6 +1.6 +0.4 +0.2
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