Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3556 and PP_3557 are separated by 246 nucleotidesPP_3557 and PP_3558 are separated by 94 nucleotidesPP_3558 and PP_3559 overlap by 4 nucleotidesPP_3559 and PP_3560 are separated by 93 nucleotides PP_3556: PP_3556 - conserved membrane protein of unknown function, at 4,033,384 to 4,034,703 _3556 PP_3557: PP_3557 - Methyl-accepting chemotaxis transducer, at 4,034,950 to 4,037,094 _3557 PP_3558: PP_3558 - putative Amino acid transporter, periplasmic amino acid-binding protein, at 4,037,189 to 4,038,205 _3558 PP_3559: PP_3559 - glycine betaine ABC transporter (permease), at 4,038,202 to 4,039,050 _3559 PP_3560: PP_3560 - Transcriptional regulator, LysR family, at 4,039,144 to 4,040,007 _3560
Group Condition PP_3556 PP_3557 PP_3558 PP_3559 PP_3560
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.4 -0.1 -0.4 -0.8 -1.2
stress R2A with Fusaric 240 ug/mL -0.1 +0.1 -0.3 -1.7 -0.2
pH Growth at pH6 and (C) D-Glucose +0.0 -0.1 -0.0 -0.7 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 +0.0 -0.5 -0.7 -0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 -0.3 -0.3 -1.4 -0.3
toxin pUC18_phdP1_pSU18T_docP1 +0.0 -0.4 -0.3 -1.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.4 -0.1 -1.1 -0.1
nitrogen source nitrogen source nitrate 5mM +0.1 -0.4 +0.2 -1.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 +0.3 +0.2 -0.6 -0.6
pH Growth at pH7 and (C) D-Glucose -0.2 -0.8 -0.3 +0.8 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.0 +0.3 +0.3 -1.1 -0.2
carbon source D-Glucose (C) +0.0 -0.1 +0.3 -1.5 +0.7
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.1 -0.3 -0.4 +0.6 -0.4
carbon source Myristic (C) +0.3 +0.1 -0.1 -1.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 +0.3 +0.2 +0.5 -0.6
carbon source Tween 20 (C) +0.3 -0.9 +0.3 +0.5 -0.1
nitrogen source 2,6-diaminopimelic (N) -0.4 -0.5 +0.3 +0.7 -0.0
stress Growth with Chloride 500 mM; with MOPS +0.1 -0.5 +0.2 +0.7 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.4 +0.6 -0.8 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.3 +0.1 +0.8 -0.9
nitrogen source NAG (N); with MOPS +0.1 -0.1 +0.4 +0.9 -0.8
phage P. putida South MOI 10 -0.2 -0.3 -0.0 +0.9 +0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.5 -0.2 +0.1 +1.3 +0.3
nitrogen source nitrate (N) -0.1 -0.3 +0.3 +0.6 +0.6
phage P. putida Sci Big MOI 0.1 +0.1 -0.4 +0.4 +0.8 +0.2
nitrogen source Urea (N); with MOPS +0.1 +0.2 +0.7 +0.9 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 +0.2 +0.3 +1.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.2 +0.0 +1.1 +0.9
phage P. putida Missley MOI 0.1 +0.0 +0.2 +0.6 +1.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 -0.1 +0.2 +2.5 -0.4
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