Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3546 and PP_3547 are separated by 7 nucleotidesPP_3547 and PP_3548 are separated by 667 nucleotidesPP_3548 and PP_3549 overlap by 4 nucleotidesPP_3549 and PP_3550 are separated by 30 nucleotides PP_3546: PP_3546 - Sensory box histidine kinase/response regulator, at 4,019,290 to 4,021,356 _3546 PP_3547: PP_3547 - putative NAD(P)-binding oxidoreductase with NAD(P)-binding Rossmann-fold domain, at 4,021,364 to 4,022,305 _3547 PP_3548: PP_3548 - multidrug efflux transport system - permease subunit, at 4,022,973 to 4,024,508 _3548 PP_3549: PP_3549 - multidrug efflux transport system - membrane fusion protein, at 4,024,505 to 4,025,698 _3549 PP_3550: PP_3550 - putative Transcriptional regulator EmrR, at 4,025,729 to 4,026,202 _3550
Group Condition PP_3546 PP_3547 PP_3548 PP_3549 PP_3550
nitrogen source NAG (N); with MOPS -0.3 +0.0 -0.3 -0.5 N.D.
temperature Growth at 30C; with MOPS -0.6 -0.1 -0.4 +0.1 N.D.
phage P. putida PUT 7 MOI 1 -0.1 -0.2 +0.1 -0.7 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.3 +0.3 -0.1 -0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.8 -0.2 -0.0 +0.2 N.D.
phage P. putida Sci small MOI 0.1 +0.1 +0.3 -0.1 -0.8 N.D.
nitrogen source nitrogen source nitrite 1mM -0.4 -0.5 -0.1 +0.5 N.D.
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 -0.5 -0.1 +0.4 N.D.
nitrogen source Urea (N); with MOPS +0.2 -0.1 +0.2 -0.7 N.D.
nitrogen source Caprolactam (N) -0.3 -0.0 -0.3 +0.4 N.D.
pH Growth at pH6 and (C) D-Glucose -0.7 +0.4 +0.2 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.5 -0.2 +0.4 N.D.
phage P. putida Missley MOI 0.1 +0.1 +0.4 +0.1 -0.8 N.D.
carbon source Valeric (C) +0.6 -0.1 -0.1 -0.5 N.D.
carbon source Ethyl decanoate (C) -0.3 -0.3 +0.1 +0.4 N.D.
pH pH 8 -0.4 -0.3 +0.2 +0.4 N.D.
temperature shift Temperature shift 30_to_25; with MOPS +0.1 +0.2 +0.2 -0.6 N.D.
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.4 -0.2 -0.2 +0.7 N.D.
carbon source ferulate (C); with MOPS +0.5 -0.2 -0.1 -0.1 N.D.
carbon source Hexanoic (C) +0.6 -0.1 -0.2 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.3 +0.2 -0.1 N.D.
carbon source butyrate (C) +0.7 -0.1 +0.0 -0.3 N.D.
phage P. putida Sci Big MOI 0.1 -0.6 +0.5 -0.1 +0.6 N.D.
phage P. putida South MOI 10 +0.3 -0.4 +0.1 +0.4 N.D.
nitrogen source 2,6-diaminopimelic (N) -0.3 -0.2 +0.2 +0.7 N.D.
nitrogen source D-2-Aminobutyric (N) -0.4 +0.1 +0.2 +0.5 N.D.
carbon source 2-Methylbutanoic (C) +0.6 -0.3 -0.1 +0.4 N.D.
nitrogen source 2,6-diaminopimelic (N) -0.3 -0.0 +0.2 +0.9 N.D.
pH Growth at pH7 and (C) D-Glucose +0.1 +0.5 -0.2 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 +0.3 -0.1 +0.4 N.D.
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