Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3540 and PP_3541 are separated by 532 nucleotidesPP_3541 and PP_3542 are separated by 168 nucleotidesPP_3542 and PP_3543 are separated by 121 nucleotidesPP_3543 and PP_3544 are separated by 50 nucleotides PP_3540: PP_3540 - Hydroxymethylglutaryl-CoA lyase, at 4,012,165 to 4,013,064 _3540 PP_3541: PP_3541 - Transporter, MgtC family, at 4,013,597 to 4,014,115 _3541 PP_3542: PP_3542 - conserved protein of unknown function, at 4,014,284 to 4,014,523 _3542 PP_3543: PP_3543 - Iron-sulfur cluster-binding protein, at 4,014,645 to 4,016,048 _3543 PP_3544: PP_3544 - Transcriptional regulator, GntR family, at 4,016,099 to 4,017,508 _3544
Group Condition PP_3540 PP_3541 PP_3542 PP_3543 PP_3544
pH Growth at pH6 and (C) D-Glucose N.D. -5.1 N.D. +0.1 +0.4
pH Growth at pH6 and (C) Trisodium citrate N.D. -2.7 N.D. -0.1 -0.2
pH Valerolactone (C) pH 6.5 N.D. -2.3 N.D. -0.1 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -2.8 N.D. +0.1 -0.1
stress Growth with Chloride 500 mM; with MOPS N.D. -2.0 N.D. +0.0 -0.4
biofilm mediium pdms biofilm mediium PDMS; 7 hours N.D. -2.3 N.D. +0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.6 N.D. -0.3 -0.3
nitrogen source NAG (N); with MOPS N.D. -1.9 N.D. +0.4 -0.3
nitrogen source NAG (N); with MOPS N.D. -1.6 N.D. +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -1.3 N.D. -0.2 -0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Phenylalanine 5 mM N.D. -1.3 N.D. +0.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.4 N.D. -0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.3 N.D. -0.4 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -1.8 N.D. +0.2 +0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. -1.5 N.D. -0.1 +0.1
carbon source Acetate Carbon Source (20mM) N.D. -1.5 N.D. +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.3 N.D. +0.4 -0.3
stress Cholinium alpha ketoglutarate 10 vol% N.D. -1.4 N.D. +0.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -1.0 N.D. +0.3 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -1.3 N.D. +0.6 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 N.D. +0.6 -0.3
carbon source Valerolactone (C) N.D. +1.3 N.D. -0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.1 N.D. -0.2 -0.2
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. +0.9 N.D. -0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +1.1 N.D. -0.3 -0.2
stress Geraniol 1 vol% N.D. -0.5 N.D. +0.3 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.7 N.D. +0.4 -0.4
carbon source Nonanoic (C) N.D. +1.5 N.D. +0.1 -0.1
carbon source Valeric (C) N.D. +1.1 N.D. +0.1 +0.3
nitrogen source 2,6-diaminopimelic (N) N.D. +1.1 N.D. +0.2 +0.2
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