Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3532 and PP_3533 are separated by 187 nucleotidesPP_3533 and PP_3534 are separated by 71 nucleotidesPP_3534 and PP_3535 are separated by 44 nucleotidesPP_3535 and PP_3536 are separated by 206 nucleotides PP_3532: PP_3532 - Transcriptional regulator, AsnC family, at 4,003,425 to 4,003,847 _3532 PP_3533: PP_3533 - putative ornithine cyclodeaminase, at 4,004,035 to 4,005,087 _3533 PP_3534: PP_3534 - Transcriptional regulator, LysR family, at 4,005,159 to 4,006,040 _3534 PP_3535: PP_3535 - putative gamma-glutamyltransferase, at 4,006,085 to 4,007,875 _3535 PP_3536: PP_3536 - conserved protein of unknown function, at 4,008,082 to 4,009,170 _3536
Group Condition PP_3532 PP_3533 PP_3534 PP_3535 PP_3536
nitrogen source NAG (N); with MOPS -6.6 -0.6 +0.3 +0.2 -0.1
nitrogen source 2,6-diaminopimelic (N) -4.1 -0.0 -0.3 -0.2 +0.1
pH Growth at pH6 and (C) Trisodium citrate -3.4 +0.1 +0.1 +0.0 -0.2
carbon source 1,3-Butandiol (C) -3.2 -0.2 +0.2 +0.0 +0.0
carbon source Valeric (C) -3.1 -0.1 -0.1 +0.2 +0.1
carbon source Trisodium citrate (C) -1.9 -0.0 -0.5 -0.5 +0.2
temperature shift Temperature shift 10_to_25; with MOPS -1.9 +0.0 -0.4 -0.1 -0.2
r2a control R2A control -2.0 +0.1 -0.2 -0.0 -0.3
pH Growth at pH6 and (C) Trisodium citrate -2.0 +0.0 -0.0 -0.0 -0.2
temperature shift Temperature shift 10_to_21; with MOPS -1.5 -0.1 -0.4 -0.2 -0.1
nitrogen source Urea (N) -2.2 -0.2 -0.1 +0.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -1.6 -0.3 -0.2 -0.0 +0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.5 -0.4 -0.8 -0.3 -0.1
temperature shift Temperature shift 10_to_30; with MOPS -2.3 -0.2 +0.1 +0.1 +0.2
carbon source Rhamnogalacturonan - from potato (C) -2.1 -0.0 -0.2 +0.2 +0.1
pH Growth at pH8 and (C) D-Glucose -1.6 +0.3 -0.8 +0.2 +0.1
nitrogen source Urea (N); with MOPS -2.9 +0.2 +0.4 +0.1 +0.2
carbon source ferulate (C); with MOPS -1.6 +0.1 -0.5 -0.2 +0.3
temperature shift Temperature shift 10_to_30; with MOPS -2.0 +0.0 -0.1 +0.1 +0.1
phage PUT 8 MOI 1 -2.7 +0.2 -0.2 +0.1 +0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.4 +0.3 +0.2 -0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 -0.1 -0.5 -0.2 +0.7
carbon source carbon source 3-O-methyl-glucose 50 mM -0.9 -0.8 -0.1 -0.0 +0.3
carbon source L-Leucine (C) -1.9 +0.0 +0.4 -0.1 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.4 +0.3 +0.2 -0.2 -0.1
carbon source L-Leucine (C) -1.4 +0.6 +0.3 -0.4 +0.1
carbon source ferulate (C); with MOPS -1.4 +0.2 +0.4 -0.0 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +1.3 -0.4 -0.5 +0.3 -0.2
nitrogen source D-2-Aminobutyric (N) +1.2 +0.3 +0.3 +0.3 -0.2
phage P. putida Missley MOI 0.1 +1.1 +0.3 +0.3 +0.1 +0.3
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