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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3532 and PP_3533 are separated by 187 nucleotides
PP_3533 and PP_3534 are separated by 71 nucleotides
PP_3534 and PP_3535 are separated by 44 nucleotides
PP_3535 and PP_3536 are separated by 206 nucleotides
PP_3532: PP_3532 - Transcriptional regulator, AsnC family, at 4,003,425 to 4,003,847
_3532
PP_3533: PP_3533 - putative ornithine cyclodeaminase, at 4,004,035 to 4,005,087
_3533
PP_3534: PP_3534 - Transcriptional regulator, LysR family, at 4,005,159 to 4,006,040
_3534
PP_3535: PP_3535 - putative gamma-glutamyltransferase, at 4,006,085 to 4,007,875
_3535
PP_3536: PP_3536 - conserved protein of unknown function, at 4,008,082 to 4,009,170
_3536
Group
Condition
PP
_3532
PP
_3533
PP
_3534
PP
_3535
PP
_3536
nitrogen source
NAG (N); with MOPS
-6.6
-0.6
+0.3
+0.2
-0.1
nitrogen source
2,6-diaminopimelic (N)
-4.1
-0.0
-0.3
-0.2
+0.1
pH
Growth at pH6 and (C) Trisodium citrate
-3.4
+0.1
+0.1
+0.0
-0.2
carbon source
1,3-Butandiol (C)
-3.2
-0.2
+0.2
+0.0
+0.0
carbon source
Valeric (C)
-3.1
-0.1
-0.1
+0.2
+0.1
carbon source
Trisodium citrate (C)
-1.9
-0.0
-0.5
-0.5
+0.2
temperature shift
Temperature shift 10_to_25; with MOPS
-1.9
+0.0
-0.4
-0.1
-0.2
r2a control
R2A control
-2.0
+0.1
-0.2
-0.0
-0.3
pH
Growth at pH6 and (C) Trisodium citrate
-2.0
+0.0
-0.0
-0.0
-0.2
temperature shift
Temperature shift 10_to_21; with MOPS
-1.5
-0.1
-0.4
-0.2
-0.1
nitrogen source
Urea (N)
-2.2
-0.2
-0.1
+0.1
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-1.6
-0.3
-0.2
-0.0
+0.1
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
-0.5
-0.4
-0.8
-0.3
-0.1
temperature shift
Temperature shift 10_to_30; with MOPS
-2.3
-0.2
+0.1
+0.1
+0.2
carbon source
Rhamnogalacturonan - from potato (C)
-2.1
-0.0
-0.2
+0.2
+0.1
pH
Growth at pH8 and (C) D-Glucose
-1.6
+0.3
-0.8
+0.2
+0.1
nitrogen source
Urea (N); with MOPS
-2.9
+0.2
+0.4
+0.1
+0.2
carbon source
ferulate (C); with MOPS
-1.6
+0.1
-0.5
-0.2
+0.3
temperature shift
Temperature shift 10_to_30; with MOPS
-2.0
+0.0
-0.1
+0.1
+0.1
phage
PUT 8 MOI 1
-2.7
+0.2
-0.2
+0.1
+0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.4
+0.3
+0.2
-0.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.5
-0.1
-0.5
-0.2
+0.7
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.9
-0.8
-0.1
-0.0
+0.3
carbon source
L-Leucine (C)
-1.9
+0.0
+0.4
-0.1
+0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.4
+0.3
+0.2
-0.2
-0.1
carbon source
L-Leucine (C)
-1.4
+0.6
+0.3
-0.4
+0.1
carbon source
ferulate (C); with MOPS
-1.4
+0.2
+0.4
-0.0
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+1.3
-0.4
-0.5
+0.3
-0.2
nitrogen source
D-2-Aminobutyric (N)
+1.2
+0.3
+0.3
+0.3
-0.2
phage
P. putida Missley MOI 0.1
+1.1
+0.3
+0.3
+0.1
+0.3
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