Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3529 and PP_3530 are separated by 133 nucleotidesPP_3530 and PP_3531 are separated by 89 nucleotidesPP_3531 and PP_3532 are separated by 82 nucleotidesPP_3532 and PP_3533 are separated by 187 nucleotides PP_3529: PP_3529 - dimethyl-sulfide monooxygenase, at 4,000,319 to 4,001,683 _3529 PP_3530: PP_3530 - putative S-ureidoglycine aminohydrolase, at 4,001,817 to 4,002,653 _3530 PP_3531: PP_3531 - conserved exported protein of unknown function, at 4,002,743 to 4,003,342 _3531 PP_3532: PP_3532 - Transcriptional regulator, AsnC family, at 4,003,425 to 4,003,847 _3532 PP_3533: PP_3533 - putative ornithine cyclodeaminase, at 4,004,035 to 4,005,087 _3533
Group Condition PP_3529 PP_3530 PP_3531 PP_3532 PP_3533
nitrogen source NAG (N); with MOPS +0.1 +0.5 +0.4 -6.6 -0.6
nitrogen source 2,6-diaminopimelic (N) +0.3 -0.3 -1.3 -4.1 -0.0
carbon source 1,3-Butandiol (C) -0.1 -0.7 -0.2 -3.2 -0.2
carbon source Valeric (C) -0.4 -0.8 +0.2 -3.1 -0.1
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.4 -0.1 -3.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -2.3 +0.0 -1.5 -0.1
carbon source ferulate (C); with MOPS -0.7 -1.6 +0.2 -1.6 +0.1
nitrogen source Urea (N) +0.1 -1.0 +0.1 -2.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.1 -0.5 -0.8 -0.7 -0.5
nitrogen source Adenine (N) +0.0 -3.0 +0.0 +0.3 +0.0
phage PUT 8 MOI 1 -0.5 +0.0 +0.4 -2.7 +0.2
carbon source Trisodium citrate (C) -0.2 -0.7 +0.4 -1.9 -0.0
temperature shift Temperature shift 10_to_30; with MOPS +0.1 -0.3 +0.2 -2.3 -0.2
nitrogen source NAG (N); with MOPS +0.2 -0.3 -0.9 -1.2 -0.3
r2a control R2A control -0.1 -0.5 +0.2 -2.0 +0.1
nitrogen source Urea (N); with MOPS +0.2 -0.3 +0.6 -2.9 +0.2
pH Growth at pH6 and (C) Trisodium citrate -0.0 -0.6 +0.4 -2.0 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +0.3 -0.7 -1.4 +0.3
carbon source ferulate (C); with MOPS +0.2 -1.3 +0.2 -1.4 +0.2
carbon source Rhamnogalacturonan - from potato (C) +0.2 -0.1 +0.1 -2.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.9 +0.4 -1.2 +0.2
nitrogen source Adenine (N) -0.0 -2.5 +0.2 +0.7 +0.1
carbon source 5-Aminovaleric 10 mM (C) -0.1 -0.5 +0.3 -1.4 +0.2
carbon source carbon source 3-O-methyl-glucose 10 mM -0.2 -0.1 +0.8 -1.4 -0.3
temperature shift Temperature shift 10_to_25; with MOPS +0.1 +0.3 +0.3 -1.9 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 -0.8 -0.7 +0.7 -0.4
carbon source L-Leucine (C) +0.0 +0.1 +0.6 -1.4 +0.6
pH pH 6 -0.1 +0.7 +0.5 -1.2 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 -0.7 -0.3 +1.0 +0.4
nitrogen source D-2-Aminobutyric (N) -0.0 -0.3 +1.1 +1.2 +0.3
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