Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3525 and PP_3526 are separated by 152 nucleotidesPP_3526 and PP_3527 are separated by 38 nucleotidesPP_3527 and PP_3528 are separated by 271 nucleotidesPP_3528 and PP_3529 are separated by 16 nucleotides PP_3525: PP_3525 - conserved exported protein of unknown function, at 3,996,683 to 3,997,162 _3525 PP_3526: PP_3526 - Transcriptional regulator, AraC family, at 3,997,315 to 3,998,322 _3526 PP_3527: PP_3527 - Transcriptional regulator, TetR family, at 3,998,361 to 3,999,044 _3527 PP_3528: PP_3528 - putative ABC transporter, periplasmic substrate-binding protein, at 3,999,316 to 4,000,302 _3528 PP_3529: PP_3529 - dimethyl-sulfide monooxygenase, at 4,000,319 to 4,001,683 _3529
Group Condition PP_3525 PP_3526 PP_3527 PP_3528 PP_3529
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.2 +0.1 -4.7 +0.2 +0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM +0.0 -0.1 -3.7 -0.1 +0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.1 -0.1 -3.6 +0.0 +0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -0.4 +0.2 -3.3 -0.1 +0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -0.1 +0.1 -3.6 -0.1 +0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM +0.2 -0.1 -3.0 -0.3 -0.1
carbon source ferulate (C); with MOPS +0.3 -0.2 -1.1 -0.6 -0.7
nitrogen source NAG (N); with MOPS -0.5 -1.1 -0.1 -0.5 +0.1
carbon source D-Glucose (C); with MOPS -1.2 -0.9 -0.1 +0.0 +0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.7 -0.1 -0.8 -0.5 +0.1
pH Growth at pH6 and (C) D-Glucose -1.4 -0.2 +0.1 +0.0 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.9 -0.5 +0.4 -0.6 -0.1
temperature Growth at 30C; with MOPS -1.1 -0.4 +0.1 -0.3 +0.0
carbon source carbon source 4-vinylphenol 0.01 mM -1.2 -0.0 -0.1 -0.1 -0.3
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.9 -0.0 -0.1 -0.6 -0.1
carbon source L-Phenylalanine 5 mM (C) -0.1 -0.2 -1.9 +0.3 +0.2
carbon source L-Phenylalanine 5 mM (C) +0.1 -0.1 -1.8 +0.2 +0.0
carbon source carbon source 3-O-methyl-glucose 50 mM -1.2 -0.2 +0.0 +0.4 -0.3
carbon source L-Phenylalanine 5 mM (C) +0.3 +0.1 -1.9 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.1 -0.5 -0.6 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.6 -0.4 +0.2 +0.1 -0.4
nitrogen source D-2-Aminobutyric (N) +0.4 -0.3 -0.9 -0.2 -0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM -0.1 +0.1 -1.3 +0.2 +0.2
nitrogen source D-2-Aminobutyric (N) -0.3 +0.1 -0.9 -0.2 +0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 -0.5 -0.2 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -0.0 -0.5 -0.6 +0.4
carbon source Vanillin (C) +0.3 +0.1 -0.9 +0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -0.3 +0.3 -0.5 +0.2
nitrogen source 2,6-diaminopimelic (N) +1.1 -0.3 -0.7 -0.4 +0.3
carbon source Protocatechuic Acid (C) +0.1 +0.4 +0.9 +0.2 -0.1
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