Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3522 and PP_3523 are separated by 158 nucleotidesPP_3523 and PP_3524 are separated by 44 nucleotidesPP_3524 and PP_3525 overlap by 17 nucleotidesPP_3525 and PP_3526 are separated by 152 nucleotides PP_3522: PP_3522 - conserved protein of unknown function, at 3,995,385 to 3,995,807 _3522 PP_3523: PP_3523 - conserved protein of unknown function, at 3,995,966 to 3,996,322 _3523 PP_3524: PP_3524 - conserved protein of unknown function, at 3,996,367 to 3,996,699 _3524 PP_3525: PP_3525 - conserved exported protein of unknown function, at 3,996,683 to 3,997,162 _3525 PP_3526: PP_3526 - Transcriptional regulator, AraC family, at 3,997,315 to 3,998,322 _3526
Group Condition PP_3522 PP_3523 PP_3524 PP_3525 PP_3526
nitrogen source 2,6-diaminopimelic (N) -3.4 -0.2 -0.9 +0.1 -0.2
temperature Growth at 30C; with MOPS -2.7 +0.0 +0.2 -1.1 -0.4
nitrogen source NAG (N); with MOPS -1.6 -0.5 +0.1 -0.8 -0.4
carbon source D-Glucose (C); with MOPS -0.8 -0.1 +0.0 -1.2 -0.9
nitrogen source D-2-Aminobutyric (N) -2.6 +0.3 -0.1 -0.3 +0.1
carbon source ferulate (C); with MOPS +0.2 -1.1 -1.2 +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.9 -0.5 +0.4 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.0 -0.0 -0.6 +0.3 -0.6
carbon source Trisodium citrate (C) -2.2 -0.6 +0.5 +0.2 +0.3
pH Growth at pH6 and (C) D-Glucose +0.2 -0.6 +0.1 -1.4 -0.2
carbon source carbon source 4-vinylphenol 0.01 mM -0.9 -0.1 +0.3 -1.2 -0.0
carbon source Myristic (C) -2.0 +0.0 -0.5 +0.4 +0.3
pH Growth at pH6 and (C) D-Glucose +0.4 -1.5 -0.4 -0.1 -0.1
stress Growth with Chloride 500 mM; with MOPS -1.4 -0.0 +0.4 -0.1 -0.5
carbon source D-Glucose (C) +0.5 -0.4 -0.9 -0.1 -0.5
nitrogen source NAG (N); with MOPS -0.4 +0.3 +0.4 -0.5 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.3 -0.4 +0.4 +0.3 -0.3
pH Growth at pH7 and (C) Trisodium citrate +0.3 -1.3 +0.4 -0.5 -0.0
carbon source carbon source 3-O-methyl-glucose 50 mM +0.5 -0.4 +0.3 -1.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.9 +0.7 -0.5 -0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +1.0 -0.2 -0.5 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.2 -0.5 -1.1 -0.0 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 +0.5 +0.2 -0.3 +0.5
nitrogen source 2,6-diaminopimelic (N) +0.3 -0.5 -0.4 +1.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 +0.8 +0.4 +0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 +1.0 +0.6 -0.1 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.6 +1.0 -0.5 +0.6 -0.4
phage P. putida Sci small MOI 0.1 -1.0 +1.0 +1.3 -0.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.4 +0.3 +0.8 +0.7 +0.3
phage P. putida Sci Big MOI 0.1 +0.9 +0.9 -0.1 +0.4 -0.2
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