Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3516 and PP_3517 are separated by 60 nucleotidesPP_3517 and PP_3518 overlap by 4 nucleotidesPP_3518 and PP_3519 are separated by 245 nucleotidesPP_3519 and PP_3520 are separated by 81 nucleotides PP_3516: PP_3516 - Transcriptional regulator, AraC family, at 3,989,716 to 3,990,672 _3516 PP_3517: PP_3517 - conserved exported protein of unknown function, at 3,990,733 to 3,991,626 _3517 PP_3518: PP_3518 - conserved exported protein of unknown function, at 3,991,623 to 3,993,068 _3518 PP_3519: PP_3519 - putative Lipoprotein, at 3,993,314 to 3,993,565 _3519 PP_3520: PP_3520 - conserved exported protein of unknown function, at 3,993,647 to 3,994,366 _3520
Group Condition PP_3516 PP_3517 PP_3518 PP_3519 PP_3520
carbon source Tween 20 (C) -0.1 -0.8 -0.1 -5.3 +0.0
temperature shift Temperature shift 30_to_21; with MOPS -0.3 -0.1 -0.2 -4.8 -0.3
carbon source D-Glucose (C); with MOPS -0.0 +0.3 -0.4 -5.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 +0.1 +0.1 -5.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.5 +0.2 +0.1 -4.7 -0.0
carbon source acetate (C) +0.1 +0.1 +0.2 -5.0 -0.3
carbon source D-Glucose (C); with MOPS -0.3 +0.4 +0.0 -5.0 +0.1
nitrogen source Valerolactam (N) -4.7 +0.1 +0.2 -0.2 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 -0.2 -0.2 -4.4 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 +0.1 +0.0 -4.4 -0.0
nitrogen source Valerolactam (N) -3.9 +0.0 -0.0 -0.7 +0.2
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL +0.1 -0.3 +0.0 -3.5 -0.1
stress NaCl stress 250 mM -0.3 -0.1 +0.1 -3.1 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.1 -0.1 +0.2 -2.7 +0.2
reactor_pregrowth Adaptation in M9 media timecourse 3; day 3 -0.3 -0.1 +0.1 -2.8 +0.0
reactor_pregrowth Adaptation in M9 media timecourse 1; day 3 -0.1 -0.0 +0.1 -3.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -0.0 +0.1 -2.6 -0.2
pH Valerolactone (C) pH 6.5 -0.3 +0.1 -0.2 -2.2 -0.3
pH LB 0.1x with 55.5 mM D-Glucose; pH 4.6 -0.0 -0.0 +0.0 -2.7 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.0 -0.4 +0.2 -2.5 +0.1
carbon source Crotonic (C) -0.1 -0.1 +0.0 -2.7 +0.2
phage P. putida Sci small MOI 0.1 +0.5 +0.6 +0.1 -3.9 +0.3
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -0.1 -0.3 +0.3 -2.3 +0.2
carbon source Nonanoic (C) +0.4 +0.2 -0.3 -2.0 -0.0
temperature Growth at 30C -0.2 +0.3 +0.1 -2.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.5 +0.5 +0.0 -1.7 +0.2
carbon source ferulate (C); with MOPS -0.6 +0.1 +0.0 +1.9 -0.3
nitrogen source D-2-Aminobutyric (N) -0.2 -0.3 -0.1 +2.9 +0.1
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.2 +0.2 +0.2 +2.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -0.3 +0.3 +5.5 +0.4
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