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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3474 and PP_3475 overlap by 4 nucleotides
PP_3475 and PP_3476 overlap by 29 nucleotides
PP_3476 and PP_3477 overlap by 7 nucleotides
PP_3477 and PP_3478 overlap by 4 nucleotides
PP_3474: PP_3474 - putative Curli fiber protein CsgE, at 3,940,271 to 3,940,672
_3474
PP_3475: PP_3475 - putative type II secretory pathway, pseudopilin pulG-related signal peptide protein, at 3,940,669 to 3,941,199
_3475
PP_3476: PP_3476 - Type II secretion system protein G, at 3,941,171 to 3,941,557
_3476
PP_3477: PP_3477 - Putative secretion type II protein related to protein G, at 3,941,551 to 3,942,030
_3477
PP_3478: PP_3478 - putative Secretion protein, at 3,942,027 to 3,943,892
_3478
Group
Condition
PP
_3474
PP
_3475
PP
_3476
PP
_3477
PP
_3478
carbon source
2,3-Butanediol (C)
-3.7
-0.3
-0.3
-1.0
-0.3
carbon source
Delta-Nonalactone (C)
-3.2
-0.5
+0.2
-0.2
-1.0
carbon source
Delta-Decalactone (C)
-2.6
-0.7
+0.1
-0.8
-0.2
carbon source
Delta-Undecalactone (C)
-3.0
-0.3
-0.1
-0.3
-0.3
carbon source
Delta-Decalactone (C)
-1.7
-0.8
-0.7
-0.4
-0.4
stress
MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
-2.4
-0.5
-0.0
-0.3
-0.1
carbon source
Decanoic (C)
-2.2
-0.3
-0.0
-0.1
-0.6
stress
R2A with Fusaric 480 ug/mL
-1.4
-0.4
-0.7
-0.4
-0.1
phage
P. putida PUT 7 MOI 1
-0.3
-1.0
-0.6
-0.1
-0.8
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-1.8
-0.0
+0.1
-1.1
+0.2
stress
R2A with Fusaric 480 ug/mL
-1.3
-0.0
-0.2
-0.5
-0.6
stress
R2A with Fusaric 480 ug/mL
-1.5
-0.2
+0.0
-0.6
-0.5
carbon source
Delta-Undecalactone (C)
-2.2
-0.1
+0.2
-0.6
+0.1
stress
pinene 10 vol%
-1.5
-0.6
+0.1
-0.5
-0.2
nitrogen source
Ammonium chloride and TNT (N)
-1.8
-0.5
+0.3
-0.6
+0.0
stress
Geraniol 1 vol%
-2.2
-0.2
+0.0
-0.1
-0.2
carbon source
ferulate (C); with MOPS
-2.3
-0.2
+0.1
-0.1
+0.1
phage
P. putida Sci small MOI 0.1
-0.1
-1.3
-0.8
+0.2
-0.4
stress
MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
-1.7
-0.1
-0.1
-0.6
+0.2
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-1.0
-0.0
-0.2
-1.3
+0.2
stress
MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
-1.7
-0.1
+0.2
-0.4
-0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-1.6
+0.2
+0.1
-0.7
-0.2
nitrogen source
Ammonium chloride and TNT (N)
-1.6
-0.5
+0.3
-0.3
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.4
-1.1
+0.6
-1.0
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.5
-0.1
-0.9
+0.9
-0.1
temperature
Growth at 25C
+0.4
+0.3
+0.2
-1.0
-0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.0
+0.0
+1.3
+0.2
-0.5
carbon source
ferulate (C); with MOPS
-1.0
+0.5
+0.2
+0.2
+0.7
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
-0.4
+1.0
+0.5
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+0.5
+0.9
+0.0
+0.8
+0.6
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