Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3450 and PP_3451 overlap by 1 nucleotidesPP_3451 and PP_3452 are separated by 124 nucleotidesPP_3452 and PP_3453 are separated by 30 nucleotidesPP_3453 and PP_3454 are separated by 6 nucleotides PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691 _3450 PP_3451: PP_3451 - conserved exported protein of unknown function, at 3,910,691 to 3,911,530 _3451 PP_3452: PP_3452 - GGDEF domain protein, at 3,911,655 to 3,912,746 _3452 PP_3453: PP_3453 - putative Sensor protein RstB, at 3,912,777 to 3,914,078 _3453 PP_3454: PP_3454 - putative transcriptional regulatory protein, at 3,914,085 to 3,914,786 _3454
Group Condition PP_3450 PP_3451 PP_3452 PP_3453 PP_3454
carbon source 2,3-Butanediol (C) +0.1 +0.5 +0.3 -2.5 -2.6
stress 2-Ethylhexanol 0.4 vol% +0.2 -0.1 -0.0 -1.4 -1.6
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -0.2 -0.5 +0.0 -0.7 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.1 -1.6 +0.0 -0.1 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -0.6 +0.1 -0.0 -1.3
carbon source Delta-Undecalactone (C) -0.0 +0.1 +0.0 -1.1 -0.9
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL +0.2 -0.0 -0.0 -0.6 -1.4
pH pH 4 -0.0 -0.4 -0.3 -1.1 +0.0
phage P. putida Sci small MOI 0.1 -0.4 -0.1 -0.3 +0.2 -1.3
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. +0.1 -0.3 -0.0 -1.4 +0.0
phage P. putida Sci Big MOI 0.1 +0.1 -1.6 -0.2 +0.1 -0.0
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -0.3 +0.1 -0.3 -0.7 -0.3
carbon source Delta-Decalactone (C) -0.1 +0.1 -0.2 -0.8 -0.6
carbon source Delta-Undecalactone (C) +0.1 +0.1 +0.0 -1.0 -0.8
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.0 -0.0 -0.1 -1.5 +0.1
carbon source carbon source 2-Deoxy-D-glucose 10 mM -0.2 +0.2 -0.5 -0.5 -0.4
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.1 -0.2 +0.3 -1.3 +0.1
carbon source Delta-Decalactone (C) -0.1 +0.4 -0.0 -0.8 -0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.3 +0.0 -1.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 +0.5 +0.1 -0.4 -0.8
phage P. putida Missley MOI 0.1 -0.6 +0.6 -0.4 -0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 -0.8 +0.5 -0.3 -0.0
stress Geraniol 1 vol% +0.4 +2.3 +0.0 -1.8 -1.4
supernatant LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter +0.2 +0.8 -0.2 -0.5 +0.3
carbon source Myristic (C) +0.3 +0.4 +0.7 +0.2 -0.5
carbon source 1-Pentanol (C) -0.1 -0.1 +0.0 +0.8 +1.0
micoeukaryotes C.elegans, mixed culture-2 +0.0 +0.6 +0.1 +0.4 +0.5
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.0 +0.4 +0.2 +0.8 +0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.0 +1.1 +0.1 +0.2 +0.9
nitrogen source 3,5-Dinitrosalicylic (N) -0.1 +0.2 +0.3 +4.7 -0.1
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