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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3449 and PP_3450 overlap by 67 nucleotides
PP_3450 and PP_3451 overlap by 1 nucleotides
PP_3451 and PP_3452 are separated by 124 nucleotides
PP_3452 and PP_3453 are separated by 30 nucleotides
PP_3449: PP_3449 - conserved exported protein of unknown function, at 3,907,687 to 3,908,118
_3449
PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691
_3450
PP_3451: PP_3451 - conserved exported protein of unknown function, at 3,910,691 to 3,911,530
_3451
PP_3452: PP_3452 - GGDEF domain protein, at 3,911,655 to 3,912,746
_3452
PP_3453: PP_3453 - putative Sensor protein RstB, at 3,912,777 to 3,914,078
_3453
Group
Condition
PP
_3449
PP
_3450
PP
_3451
PP
_3452
PP
_3453
phage
P. putida Sci small MOI 0.1
-6.2
-0.3
+0.5
-0.2
-0.3
nitrogen source
2,6-diaminopimelic (N)
-3.1
-0.2
-0.3
-0.0
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-2.0
-0.1
-0.9
+0.1
-0.3
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-1.9
-0.0
+0.1
+0.2
-1.1
carbon source
D-Glucose (C)
-1.3
-0.2
-0.8
-0.3
+0.0
temperature shift
Temperature shift 30_to_34; with MOPS
-2.1
-0.3
+0.2
-0.2
-0.2
reactor
40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
-0.8
+0.1
-0.3
-0.0
-1.4
nitrogen source
NAG (N)
-2.3
-0.0
-0.1
-0.1
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.5
-0.1
-1.6
+0.0
-0.1
carbon source
2,3-Butanediol (C)
-0.7
+0.1
+0.5
+0.3
-2.5
no stress control
Growth with no stress
-1.6
-0.2
-0.1
-0.3
-0.1
nitrogen source
Urea (N)
-2.3
-0.0
+0.1
-0.1
+0.1
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
-1.6
+0.1
-0.3
+0.1
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.9
+0.0
-0.9
+0.0
-0.3
carbon source
carbon source 2-Deoxy-D-glucose 10 mM
-1.0
-0.2
+0.2
-0.5
-0.5
nitrogen source
nitrate (N)
-1.2
-0.2
-0.3
-0.4
+0.1
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
-0.4
-0.1
-0.2
+0.3
-1.3
temperature shift
Temperature shift 10_to_30; with MOPS
-1.7
+0.1
+0.1
-0.3
+0.2
temperature shift
Temperature shift 10_to_30
-1.9
+0.0
+0.4
-0.1
+0.1
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.9
+0.0
+0.5
-0.5
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.7
+0.1
-0.8
+0.5
-0.3
nitrogen source
Propandiamine (N)
-2.3
+0.0
+0.9
+0.2
+0.0
nitrogen source
NAG (N); with MOPS
-1.1
-0.3
+0.4
+0.3
+0.1
pH
Growth at pH9 and (C) Trisodium citrate
-1.1
-0.1
+0.6
-0.1
+0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.7
+0.0
+0.3
-0.3
-1.0
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-1.1
-0.0
+0.4
+0.2
+0.8
stress
Geraniol 1 vol%
+0.1
+0.4
+2.3
+0.0
-1.8
pH
Growth at pH7 and (C) Trisodium citrate
+1.2
+0.1
+0.3
-0.1
+0.5
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+2.8
+0.2
+0.1
+0.4
+0.2
nitrogen source
3,5-Dinitrosalicylic (N)
-0.3
-0.1
+0.2
+0.3
+4.7
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