Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3449 and PP_3450 overlap by 67 nucleotidesPP_3450 and PP_3451 overlap by 1 nucleotidesPP_3451 and PP_3452 are separated by 124 nucleotidesPP_3452 and PP_3453 are separated by 30 nucleotides PP_3449: PP_3449 - conserved exported protein of unknown function, at 3,907,687 to 3,908,118 _3449 PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691 _3450 PP_3451: PP_3451 - conserved exported protein of unknown function, at 3,910,691 to 3,911,530 _3451 PP_3452: PP_3452 - GGDEF domain protein, at 3,911,655 to 3,912,746 _3452 PP_3453: PP_3453 - putative Sensor protein RstB, at 3,912,777 to 3,914,078 _3453
Group Condition PP_3449 PP_3450 PP_3451 PP_3452 PP_3453
phage P. putida Sci small MOI 0.1 -6.2 -0.3 +0.5 -0.2 -0.3
nitrogen source 2,6-diaminopimelic (N) -3.1 -0.2 -0.3 -0.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -2.0 -0.1 -0.9 +0.1 -0.3
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -1.9 -0.0 +0.1 +0.2 -1.1
carbon source D-Glucose (C) -1.3 -0.2 -0.8 -0.3 +0.0
temperature shift Temperature shift 30_to_34; with MOPS -2.1 -0.3 +0.2 -0.2 -0.2
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -0.8 +0.1 -0.3 -0.0 -1.4
nitrogen source NAG (N) -2.3 -0.0 -0.1 -0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.5 -0.1 -1.6 +0.0 -0.1
carbon source 2,3-Butanediol (C) -0.7 +0.1 +0.5 +0.3 -2.5
no stress control Growth with no stress -1.6 -0.2 -0.1 -0.3 -0.1
nitrogen source Urea (N) -2.3 -0.0 +0.1 -0.1 +0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -1.6 +0.1 -0.3 +0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.9 +0.0 -0.9 +0.0 -0.3
carbon source carbon source 2-Deoxy-D-glucose 10 mM -1.0 -0.2 +0.2 -0.5 -0.5
nitrogen source nitrate (N) -1.2 -0.2 -0.3 -0.4 +0.1
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.4 -0.1 -0.2 +0.3 -1.3
temperature shift Temperature shift 10_to_30; with MOPS -1.7 +0.1 +0.1 -0.3 +0.2
temperature shift Temperature shift 10_to_30 -1.9 +0.0 +0.4 -0.1 +0.1
carbon source carbon source 3-O-methyl-glucose 50 mM -0.9 +0.0 +0.5 -0.5 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.7 +0.1 -0.8 +0.5 -0.3
nitrogen source Propandiamine (N) -2.3 +0.0 +0.9 +0.2 +0.0
nitrogen source NAG (N); with MOPS -1.1 -0.3 +0.4 +0.3 +0.1
pH Growth at pH9 and (C) Trisodium citrate -1.1 -0.1 +0.6 -0.1 +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.7 +0.0 +0.3 -0.3 -1.0
carbon source 3-methyl-1-butanol 0.1 vol% (C) -1.1 -0.0 +0.4 +0.2 +0.8
stress Geraniol 1 vol% +0.1 +0.4 +2.3 +0.0 -1.8
pH Growth at pH7 and (C) Trisodium citrate +1.2 +0.1 +0.3 -0.1 +0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.8 +0.2 +0.1 +0.4 +0.2
nitrogen source 3,5-Dinitrosalicylic (N) -0.3 -0.1 +0.2 +0.3 +4.7
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