Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3447 and PP_3448 are separated by 192 nucleotidesPP_3448 and PP_3449 overlap by 4 nucleotidesPP_3449 and PP_3450 overlap by 67 nucleotidesPP_3450 and PP_3451 overlap by 1 nucleotides PP_3447: PP_3447 - conserved membrane protein of unknown function, at 3,904,892 to 3,905,323 _3447 PP_3448: PP_3448 - bacteriophage N4 receptor, inner membrane subunit, at 3,905,516 to 3,907,690 _3448 PP_3449: PP_3449 - conserved exported protein of unknown function, at 3,907,687 to 3,908,118 _3449 PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691 _3450 PP_3451: PP_3451 - conserved exported protein of unknown function, at 3,910,691 to 3,911,530 _3451
Group Condition PP_3447 PP_3448 PP_3449 PP_3450 PP_3451
nitrogen source NAG (N); with MOPS -5.0 -0.5 -1.1 -0.3 +0.4
pH Growth at pH6 and (C) D-Glucose -5.1 -0.3 -0.5 -0.1 -0.3
pH Growth at pH7 and (C) D-Glucose -5.2 +0.0 -1.0 +0.0 +0.1
phage P. putida Sci small MOI 0.1 +0.0 -0.0 -6.2 -0.3 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -5.9 +0.3 +0.5 -0.1 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.2 -0.1 -0.9 +0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -4.1 +0.2 -0.4 -0.2 -0.0
pH Growth at pH6 and (C) D-Glucose -5.1 -0.1 +0.4 -0.1 +0.5
nitrogen source 2,6-diaminopimelic (N) -0.2 +0.5 -3.1 -0.2 -0.3
nitrogen source nitrogen source nitrite 1mM -2.4 -0.0 -0.8 -0.2 -0.0
carbon source carbon source D-Glucose 55.5 mM -3.2 -0.2 -0.0 -0.2 +0.2
carbon source ferulate (C); with MOPS -2.5 +0.1 -0.7 -0.2 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.1 +0.0 -2.0 -0.1 -0.9
carbon source L-Leucine (C) -2.0 -0.3 -0.9 +0.1 +0.1
phage P. putida Sci Big MOI 0.1 -1.5 +0.1 +0.0 +0.1 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -2.4 +0.1 -0.2 -0.3 +0.2
carbon source Delta-Undecalactone (C) -3.0 +0.1 +0.3 -0.0 +0.1
carbon source Valerolactone (C) -3.3 +0.3 -0.1 +0.3 +0.6
nitrogen source Urea (N) +0.5 -0.3 -2.3 -0.0 +0.1
temperature shift Temperature shift 30_to_34; with MOPS +0.7 +0.1 -2.1 -0.3 +0.2
stress R2A with Fusaric 240 ug/mL +1.1 -0.5 -1.6 -0.1 -0.3
nitrogen source Propandiamine (N) +0.3 +0.1 -2.3 +0.0 +0.9
carbon source D-Glucose (C) +1.6 -0.0 -1.3 -0.2 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-2 -1.6 +0.1 -0.4 +0.0 +1.1
nitrogen source NAG (N) +1.9 +0.0 -2.3 -0.0 -0.1
r2a control with 0.2% methanol R2A control with 0.2% methanol +1.4 -0.1 -1.1 -0.0 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.2 +0.5 -0.7 -0.2 -0.7
carbon source Trisodium citrate (C) +1.8 -0.2 +0.9 +0.1 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.4 +2.8 +0.2 +0.1
temperature Growth at 25C; with MOPS +1.8 -0.2 +1.2 -0.1 +0.3
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