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Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_3447 and PP_3448 are separated by 192 nucleotides
PP_3448 and PP_3449 overlap by 4 nucleotides
PP_3449 and PP_3450 overlap by 67 nucleotides
PP_3450 and PP_3451 overlap by 1 nucleotides
PP_3447: PP_3447 - conserved membrane protein of unknown function, at 3,904,892 to 3,905,323
_3447
PP_3448: PP_3448 - bacteriophage N4 receptor, inner membrane subunit, at 3,905,516 to 3,907,690
_3448
PP_3449: PP_3449 - conserved exported protein of unknown function, at 3,907,687 to 3,908,118
_3449
PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691
_3450
PP_3451: PP_3451 - conserved exported protein of unknown function, at 3,910,691 to 3,911,530
_3451
Group
Condition
PP
_3447
PP
_3448
PP
_3449
PP
_3450
PP
_3451
nitrogen source
NAG (N); with MOPS
-5.0
-0.5
-1.1
-0.3
+0.4
pH
Growth at pH6 and (C) D-Glucose
-5.1
-0.3
-0.5
-0.1
-0.3
pH
Growth at pH7 and (C) D-Glucose
-5.2
+0.0
-1.0
+0.0
+0.1
phage
P. putida Sci small MOI 0.1
+0.0
-0.0
-6.2
-0.3
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-5.9
+0.3
+0.5
-0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-4.2
-0.1
-0.9
+0.1
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-4.1
+0.2
-0.4
-0.2
-0.0
pH
Growth at pH6 and (C) D-Glucose
-5.1
-0.1
+0.4
-0.1
+0.5
nitrogen source
2,6-diaminopimelic (N)
-0.2
+0.5
-3.1
-0.2
-0.3
nitrogen source
nitrogen source nitrite 1mM
-2.4
-0.0
-0.8
-0.2
-0.0
carbon source
carbon source D-Glucose 55.5 mM
-3.2
-0.2
-0.0
-0.2
+0.2
carbon source
ferulate (C); with MOPS
-2.5
+0.1
-0.7
-0.2
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-0.1
+0.0
-2.0
-0.1
-0.9
carbon source
L-Leucine (C)
-2.0
-0.3
-0.9
+0.1
+0.1
phage
P. putida Sci Big MOI 0.1
-1.5
+0.1
+0.0
+0.1
-1.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-2.4
+0.1
-0.2
-0.3
+0.2
carbon source
Delta-Undecalactone (C)
-3.0
+0.1
+0.3
-0.0
+0.1
carbon source
Valerolactone (C)
-3.3
+0.3
-0.1
+0.3
+0.6
nitrogen source
Urea (N)
+0.5
-0.3
-2.3
-0.0
+0.1
temperature shift
Temperature shift 30_to_34; with MOPS
+0.7
+0.1
-2.1
-0.3
+0.2
stress
R2A with Fusaric 240 ug/mL
+1.1
-0.5
-1.6
-0.1
-0.3
nitrogen source
Propandiamine (N)
+0.3
+0.1
-2.3
+0.0
+0.9
carbon source
D-Glucose (C)
+1.6
-0.0
-1.3
-0.2
-0.8
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-1.6
+0.1
-0.4
+0.0
+1.1
nitrogen source
NAG (N)
+1.9
+0.0
-2.3
-0.0
-0.1
r2a control with 0.2% methanol
R2A control with 0.2% methanol
+1.4
-0.1
-1.1
-0.0
-0.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+1.2
+0.5
-0.7
-0.2
-0.7
carbon source
Trisodium citrate (C)
+1.8
-0.2
+0.9
+0.1
-0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.8
+0.4
+2.8
+0.2
+0.1
temperature
Growth at 25C; with MOPS
+1.8
-0.2
+1.2
-0.1
+0.3
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