Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3446 and PP_3447 are separated by 149 nucleotidesPP_3447 and PP_3448 are separated by 192 nucleotidesPP_3448 and PP_3449 overlap by 4 nucleotidesPP_3449 and PP_3450 overlap by 67 nucleotides PP_3446: PP_3446 - threonine deaminase, at 3,903,150 to 3,904,742 _3446 PP_3447: PP_3447 - conserved membrane protein of unknown function, at 3,904,892 to 3,905,323 _3447 PP_3448: PP_3448 - bacteriophage N4 receptor, inner membrane subunit, at 3,905,516 to 3,907,690 _3448 PP_3449: PP_3449 - conserved exported protein of unknown function, at 3,907,687 to 3,908,118 _3449 PP_3450: PP_3450 - conserved protein of unknown function containing TPR repeat, at 3,908,052 to 3,910,691 _3450
Group Condition PP_3446 PP_3447 PP_3448 PP_3449 PP_3450
nitrogen source NAG (N); with MOPS -0.2 -5.0 -0.5 -1.1 -0.3
pH Growth at pH7 and (C) D-Glucose -0.4 -5.2 +0.0 -1.0 +0.0
phage P. putida Sci small MOI 0.1 -0.1 +0.0 -0.0 -6.2 -0.3
pH Growth at pH6 and (C) D-Glucose -0.4 -5.1 -0.3 -0.5 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.1 -5.9 +0.3 +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -4.2 -0.1 -0.9 +0.1
pH Growth at pH6 and (C) D-Glucose -0.0 -5.1 -0.1 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 -4.1 +0.2 -0.4 -0.2
nitrogen source 2,6-diaminopimelic (N) -0.9 -0.2 +0.5 -3.1 -0.2
nitrogen source nitrogen source nitrite 1mM -0.3 -2.4 -0.0 -0.8 -0.2
carbon source carbon source D-Glucose 55.5 mM +0.1 -3.2 -0.2 -0.0 -0.2
carbon source ferulate (C); with MOPS +0.1 -2.5 +0.1 -0.7 -0.2
carbon source L-Leucine (C) +0.0 -2.0 -0.3 -0.9 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.0 -2.4 +0.1 -0.2 -0.3
carbon source Valerolactone (C) +0.0 -3.3 +0.3 -0.1 +0.3
carbon source Delta-Undecalactone (C) -0.0 -3.0 +0.1 +0.3 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.5 -1.6 +0.1 -0.4 +0.0
nitrogen source Urea (N) +0.1 +0.5 -0.3 -2.3 -0.0
temperature shift Temperature shift 30_to_34; with MOPS -0.2 +0.7 +0.1 -2.1 -0.3
nitrogen source Propandiamine (N) +0.1 +0.3 +0.1 -2.3 +0.0
stress R2A with Fusaric 240 ug/mL -0.0 +1.1 -0.5 -1.6 -0.1
nitrogen source NAG (N) -0.1 +1.9 +0.0 -2.3 -0.0
carbon source D-Glucose (C) -0.2 +1.6 -0.0 -1.3 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.9 +1.2 +0.5 -0.7 -0.2
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.1 +1.4 -0.1 -1.1 -0.0
carbon source ferulate (C); with MOPS -0.1 +0.8 -0.7 +1.0 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.4 +2.1 -0.1 -0.1 -0.1
carbon source Trisodium citrate (C) -0.6 +1.8 -0.2 +0.9 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.8 +0.4 +2.8 +0.2
temperature Growth at 25C; with MOPS +0.1 +1.8 -0.2 +1.2 -0.1
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