Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3421 and PP_3422 are separated by 174 nucleotidesPP_3422 and PP_3423 are separated by 101 nucleotidesPP_3423 and PP_3424 overlap by 10 nucleotidesPP_3424 and PP_3425 are separated by 191 nucleotides PP_3421: PP_3421 - Sensor histidine kinase, at 3,872,688 to 3,874,139 _3421 PP_3422: PP_3422 - Lytic transglycosylase, at 3,874,314 to 3,874,907 _3422 PP_3423: PP_3423 - Type II secretion pathway protein XcpT, at 3,875,009 to 3,875,518 _3423 PP_3424: PP_3424 - Type II secretion pathway protein XcpS, at 3,875,509 to 3,876,720 _3424 PP_3425: PP_3425 - Efflux transporter RND family, MFP subunit, at 3,876,912 to 3,878,153 _3425
Group Condition PP_3421 PP_3422 PP_3423 PP_3424 PP_3425
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 -0.0 -0.2 -0.1 -1.2
pH Valerolactone (C) pH 6.5 -0.3 +0.1 -0.1 -1.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.0 -0.3 +0.1 +0.5 -1.4
pH pH 8 +0.4 -0.2 +0.2 -0.2 -1.2
nitrogen source 2,6-diaminopimelic (N) +0.3 +0.1 -1.3 +0.1 -0.2
carbon source Nonanoic (C) -0.4 +0.3 -0.2 -0.0 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 +0.3 -0.2 +0.0 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.0 -0.1 +0.2 -1.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.4 -0.4 -0.5 +0.4
phage P. putida Sci small MOI 0.1 +0.1 +0.5 -0.4 -0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.0 -0.4 -0.5 +0.6
carbon source carbon source 3-O-methyl-glucose 50 mM +0.2 -0.0 +0.5 -0.3 -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.0 +0.0 +0.3 +0.3 -1.0
phage P. putida Sci small MOI 0.1 +0.2 +0.4 -0.0 +0.2 -1.1
pH Growth at pH6 and (C) D-Glucose +0.1 +0.1 -0.5 -0.4 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.4 -0.5 +0.1 -0.4
carbon source carbon source Glycerol 108.6 mM +0.4 -0.2 +0.5 -0.1 -0.7
nitrogen source NAG (N); with MOPS -0.0 -0.6 +0.2 -0.2 +0.7
phage P. putida South MOI 10 +0.1 -0.2 +0.3 -0.7 +0.5
pH pH 9 +0.3 +0.1 +0.7 -0.7 -0.3
pH pH 5 +0.3 -0.3 +0.5 -0.2 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.7 -0.6 -0.6 +0.6
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.3 -0.3 +0.3 +0.3 -0.4
pH pH 6 +0.4 -0.3 +0.5 +0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 -0.3 +0.3 +0.5 +0.5
carbon source Valeric (C) +0.1 +0.3 -0.5 +0.4 +0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 +0.4 +0.1 +0.8 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.5 +0.3 +0.4 +0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.8 +0.2 +0.7 +0.5 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 +0.4 +0.6 +0.6 +0.2
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