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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3400 and PP_3401 are separated by 169 nucleotides
PP_3401 and PP_3402 are separated by 81 nucleotides
PP_3402 and PP_3403 are separated by 120 nucleotides
PP_3403 and PP_3404 are separated by 96 nucleotides
PP_3400: PP_3400 - alpha-ketoglutarate- and Fe(II)-dependent dioxygenase, at 3,852,613 to 3,853,260
_3400
PP_3401: PP_3401 - conserved protein of unknown function, at 3,853,430 to 3,853,717
_3401
PP_3402: PP_3402 - conserved protein of unknown function, at 3,853,799 to 3,854,194
_3402
PP_3403: PP_3403 - putative Quercetin 2,3-dioxygenase, at 3,854,315 to 3,855,202
_3403
PP_3404: PP_3404 - conserved protein of unknown function, at 3,855,299 to 3,856,171
_3404
Group
Condition
PP
_3400
PP
_3401
PP
_3402
PP
_3403
PP
_3404
phage
P. putida Sci Big MOI 0.1
+0.0
-0.4
-0.8
-0.5
-1.0
carbon source
ferulate (C); with MOPS
-0.2
-3.5
+0.0
+0.2
+1.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.2
-2.2
-0.0
+0.3
-0.2
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-1.0
+0.2
-0.0
-0.3
-0.9
carbon source
Myristic (C)
+0.3
-1.1
-0.0
-0.6
-0.8
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-0.5
-0.9
-0.1
+0.1
-0.7
phage
P. putida South MOI 10
-0.7
-0.6
-0.4
+0.2
-0.5
nitrogen source
NAG (N); with MOPS
-0.5
-1.2
-0.2
-0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.2
-1.0
-0.6
-0.5
+0.7
pH
Growth at pH7 and (C) Trisodium citrate
+0.4
-0.8
-0.1
-0.3
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-1.0
-0.6
+0.3
+0.3
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.7
-0.7
+0.0
+0.3
-0.3
carbon source
D-Glucose (C)
-1.0
+0.4
+0.3
-0.1
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.5
-0.3
+0.2
-0.7
+0.5
phage
P. putida Sci small MOI 0.1
+1.0
-0.4
-0.7
-0.2
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+0.5
-0.8
+0.2
+0.4
-1.0
pH
Growth at pH6 and (C) D-Glucose
-0.5
-0.7
+0.4
-0.1
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.7
-0.4
-0.1
+0.2
+0.7
stress
R2A with Fusaric 240 ug/mL
+0.1
-0.7
+0.2
-0.5
+0.6
temperature shift
Temperature shift 10_to_25; with MOPS
+0.2
+0.8
+0.5
+0.0
-1.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
+1.2
-0.3
-0.2
-0.4
carbon source
carbon source D-Glucose 55.5 mM
+0.8
+0.4
-0.5
-0.0
-0.7
carbon source
D-Glucose (C); with MOPS
-0.9
+0.5
-0.1
+0.2
+0.4
carbon source
4-Aminobutyric 10 mM (C)
+0.6
-0.8
-0.1
+0.4
+0.3
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.8
+0.6
-0.7
-0.0
-0.3
pH
Valerolactone (C) pH 6.5
-0.0
-0.7
+0.0
+0.4
+1.1
carbon source
ferulate (C); with MOPS
+0.3
+0.3
-0.3
-0.2
+1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.4
-0.1
-0.2
+1.2
phage
P. putida PUT 7 MOI 1
+1.1
+0.6
+0.0
+0.0
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+1.2
+0.3
+0.4
+0.6
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