Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3400 and PP_3401 are separated by 169 nucleotidesPP_3401 and PP_3402 are separated by 81 nucleotidesPP_3402 and PP_3403 are separated by 120 nucleotidesPP_3403 and PP_3404 are separated by 96 nucleotides PP_3400: PP_3400 - alpha-ketoglutarate- and Fe(II)-dependent dioxygenase, at 3,852,613 to 3,853,260 _3400 PP_3401: PP_3401 - conserved protein of unknown function, at 3,853,430 to 3,853,717 _3401 PP_3402: PP_3402 - conserved protein of unknown function, at 3,853,799 to 3,854,194 _3402 PP_3403: PP_3403 - putative Quercetin 2,3-dioxygenase, at 3,854,315 to 3,855,202 _3403 PP_3404: PP_3404 - conserved protein of unknown function, at 3,855,299 to 3,856,171 _3404
Group Condition PP_3400 PP_3401 PP_3402 PP_3403 PP_3404
phage P. putida Sci Big MOI 0.1 +0.0 -0.4 -0.8 -0.5 -1.0
carbon source ferulate (C); with MOPS -0.2 -3.5 +0.0 +0.2 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -2.2 -0.0 +0.3 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 -1.0 +0.2 -0.0 -0.3 -0.9
carbon source Myristic (C) +0.3 -1.1 -0.0 -0.6 -0.8
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.5 -0.9 -0.1 +0.1 -0.7
phage P. putida South MOI 10 -0.7 -0.6 -0.4 +0.2 -0.5
nitrogen source NAG (N); with MOPS -0.5 -1.2 -0.2 -0.1 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 -1.0 -0.6 -0.5 +0.7
pH Growth at pH7 and (C) Trisodium citrate +0.4 -0.8 -0.1 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.0 -0.6 +0.3 +0.3 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.7 -0.7 +0.0 +0.3 -0.3
carbon source D-Glucose (C) -1.0 +0.4 +0.3 -0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -0.3 +0.2 -0.7 +0.5
phage P. putida Sci small MOI 0.1 +1.0 -0.4 -0.7 -0.2 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.5 -0.8 +0.2 +0.4 -1.0
pH Growth at pH6 and (C) D-Glucose -0.5 -0.7 +0.4 -0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 -0.4 -0.1 +0.2 +0.7
stress R2A with Fusaric 240 ug/mL +0.1 -0.7 +0.2 -0.5 +0.6
temperature shift Temperature shift 10_to_25; with MOPS +0.2 +0.8 +0.5 +0.0 -1.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +1.2 -0.3 -0.2 -0.4
carbon source carbon source D-Glucose 55.5 mM +0.8 +0.4 -0.5 -0.0 -0.7
carbon source D-Glucose (C); with MOPS -0.9 +0.5 -0.1 +0.2 +0.4
carbon source 4-Aminobutyric 10 mM (C) +0.6 -0.8 -0.1 +0.4 +0.3
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.8 +0.6 -0.7 -0.0 -0.3
pH Valerolactone (C) pH 6.5 -0.0 -0.7 +0.0 +0.4 +1.1
carbon source ferulate (C); with MOPS +0.3 +0.3 -0.3 -0.2 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.4 -0.1 -0.2 +1.2
phage P. putida PUT 7 MOI 1 +1.1 +0.6 +0.0 +0.0 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +1.2 +0.3 +0.4 +0.6
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