Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3398 and PP_3399 are separated by 16 nucleotidesPP_3399 and PP_3400 are separated by 158 nucleotidesPP_3400 and PP_3401 are separated by 169 nucleotidesPP_3401 and PP_3402 are separated by 81 nucleotides PP_3398: PP_3398 - putative Curli fiber surface-exposed nucleator CsgB, at 3,850,519 to 3,850,992 _3398 PP_3399: PP_3399 - minor curlin subunit CsgB, nucleation component of curlin monomers, at 3,851,009 to 3,852,454 _3399 PP_3400: PP_3400 - alpha-ketoglutarate- and Fe(II)-dependent dioxygenase, at 3,852,613 to 3,853,260 _3400 PP_3401: PP_3401 - conserved protein of unknown function, at 3,853,430 to 3,853,717 _3401 PP_3402: PP_3402 - conserved protein of unknown function, at 3,853,799 to 3,854,194 _3402
Group Condition PP_3398 PP_3399 PP_3400 PP_3401 PP_3402
nitrogen source Propandiamine (N) -4.5 -0.2 -0.5 +0.4 -0.1
phage P. putida Sci Big MOI 0.1 -1.6 -0.4 +0.0 -0.4 -0.8
carbon source rac-3-Hydroxypentanoic Acid (C) -2.3 -0.0 -0.3 -0.3 -0.1
carbon source rac-3-Hydroxypentanoic Acid (C) -1.9 +0.0 -0.2 -0.4 -0.1
carbon source D-Glucose (C); with MOPS -2.0 +0.3 +0.1 -0.5 -0.4
carbon source gluconate (C) -2.5 +0.0 -0.1 +0.2 -0.1
carbon source Ethyl decanoate (C) -2.3 -0.1 +0.1 +0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.3 -0.2 -2.2 -0.0
r2a control R2A control -2.1 -0.1 +0.4 -0.4 +0.0
phage P. putida Sci small MOI 0.1 -2.1 +0.1 +1.0 -0.4 -0.7
nitrogen source NAG (N); with MOPS -2.3 +0.2 +0.8 -0.0 -0.5
carbon source ferulate (C); with MOPS +1.9 +0.3 -0.2 -3.5 +0.0
carbon source 4-Aminobutyric 10 mM (C) -1.2 +0.1 +0.6 -0.8 -0.1
nitrogen source no extra (N); with MOPS -1.8 +0.1 -0.1 +0.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 +0.1 -0.2 -1.0 -0.6
nitrogen source NAG (N); with MOPS +0.9 +0.2 -0.5 -1.2 -0.2
carbon source D-Glucose (C) -1.7 +0.2 +0.5 +0.1 +0.2
nitrogen source 2,6-diaminopimelic (N) -1.2 -0.1 -0.3 +1.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 -0.2 +0.5 +0.5 -0.2
pH Growth at pH8 and (C) D-Glucose -1.6 -0.0 +0.2 +0.6 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +1.0 +0.1 -1.0 -0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.3 +0.1 -0.7 -0.4 -0.1
carbon source D-Glucose (C); with MOPS +1.4 -0.2 -0.9 +0.5 -0.1
carbon source Valerolactone (C) -0.6 +0.2 +0.8 +0.8 -0.2
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.3 +0.5 +0.8 +0.6 -0.7
pH pH 6 +1.3 +0.1 +0.8 -0.3 -0.3
carbon source carbon source D-Glucose 55.5 mM +0.7 +0.3 +0.8 +0.4 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +1.7 +0.3 +0.5 -0.8 +0.2
pH pH 9 +1.4 +0.5 +0.7 -0.2 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +2.2 +0.1 +0.3 -0.4 +0.5
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