Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3376 and PP_3377 are separated by 10 nucleotidesPP_3377 and PP_3378 are separated by 55 nucleotidesPP_3378 and PP_3379 overlap by 8 nucleotidesPP_3379 and PP_3380 are separated by 64 nucleotides PP_3376: PP_3376 - putative phosphonate dehydrogenase, at 3,820,918 to 3,821,880 _3376 PP_3377: PP_3377 - 2-ketogluconate transporter, putative, at 3,821,891 to 3,823,183 _3377 PP_3378: PP_3378 - putative 2-ketogluconokinase, at 3,823,239 to 3,824,189 _3378 PP_3379: PP_3379 - putative epimerase, at 3,824,182 to 3,824,961 _3379 PP_3380: PP_3380 - 2-ketogluconate utilization repressor, at 3,825,026 to 3,826,045 _3380
Group Condition PP_3376 PP_3377 PP_3378 PP_3379 PP_3380
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -5.1 -5.3 -4.3 N.D. -5.5
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -4.5 -6.0 -4.2 N.D. -3.8
nitrogen source D-2-Aminobutyric (N) -2.0 -1.5 -2.3 N.D. -2.1
carbon source gluconate (C) -3.2 -1.6 -1.9 N.D. -0.8
temperature Growth at 25C -1.3 -0.4 -5.3 N.D. -0.2
nitrogen source DL-2-Aminobutyric (N) -2.6 -1.6 -1.0 N.D. -1.7
nitrogen source DL-2-Aminobutyric (N) -2.1 -1.4 -1.9 N.D. -1.1
nitrogen source Caprolactam (N) -1.5 -1.6 -1.0 N.D. -2.4
nitrogen source nitrite (N) -2.6 -1.5 -1.1 N.D. -0.9
pH Growth at pH6 and (C) Trisodium citrate +0.1 -0.0 -5.2 N.D. -0.9
nitrogen source nitrite (N) -2.8 -1.5 -0.9 N.D. -0.7
nitrogen source 1,6-Hexanediamine (N) -2.3 -1.3 -1.1 N.D. -1.1
stress R2A with Fusaric 240 ug/mL -0.2 -0.2 -5.2 N.D. -0.3
nitrogen source 1,6-Hexanediamine (N) -2.2 -1.3 -0.8 N.D. -1.5
nitrogen source Uracil (N) -1.9 -1.2 -1.4 N.D. -1.1
nitrogen source Uracil (N) -2.4 -1.2 -0.7 N.D. -1.1
nitrogen source Propiolactam (N) -1.3 -1.1 -1.3 N.D. -1.5
carbon source gluconate (C) -2.5 -1.2 -0.9 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -2.1 +0.2 -1.8 N.D. -1.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -4.7 +0.1 -0.2 N.D. +0.3
carbon source carbon source 4-vinylphenol 0.01 mM -4.8 +0.0 +0.4 N.D. +0.0
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -4.5 +0.2 +0.4 N.D. -0.3
pH pH 5 -3.7 -0.1 +0.6 N.D. -1.0
carbon source carbon source Glycerol 108.6 mM -5.0 -0.1 +0.9 N.D. -0.1
carbon source carbon source Bisabolol 45 mM -4.2 -0.2 +1.2 N.D. -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -3.7 +0.2 -0.4 N.D. +0.7
carbon source carbon source 2-Deoxy-D-glucose 50 mM -3.2 +0.0 +1.3 N.D. -1.3
pH pH 6 -4.5 -0.2 +1.2 N.D. +0.5
pH pH 8 -3.9 +0.1 +0.8 N.D. +0.3
pH pH 4 -3.7 -0.1 +1.2 N.D. +0.1
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