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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3376 and PP_3377 are separated by 10 nucleotides
PP_3377 and PP_3378 are separated by 55 nucleotides
PP_3378 and PP_3379 overlap by 8 nucleotides
PP_3379 and PP_3380 are separated by 64 nucleotides
PP_3376: PP_3376 - putative phosphonate dehydrogenase, at 3,820,918 to 3,821,880
_3376
PP_3377: PP_3377 - 2-ketogluconate transporter, putative, at 3,821,891 to 3,823,183
_3377
PP_3378: PP_3378 - putative 2-ketogluconokinase, at 3,823,239 to 3,824,189
_3378
PP_3379: PP_3379 - putative epimerase, at 3,824,182 to 3,824,961
_3379
PP_3380: PP_3380 - 2-ketogluconate utilization repressor, at 3,825,026 to 3,826,045
_3380
Group
Condition
PP
_3376
PP
_3377
PP
_3378
PP
_3379
PP
_3380
carbon source
2-Keto-D-gluconic hemicalcium salt (C)
-5.1
-5.3
-4.3
N.D.
-5.5
carbon source
2-Keto-D-gluconic hemicalcium salt (C)
-4.5
-6.0
-4.2
N.D.
-3.8
nitrogen source
D-2-Aminobutyric (N)
-2.0
-1.5
-2.3
N.D.
-2.1
carbon source
gluconate (C)
-3.2
-1.6
-1.9
N.D.
-0.8
temperature
Growth at 25C
-1.3
-0.4
-5.3
N.D.
-0.2
nitrogen source
DL-2-Aminobutyric (N)
-2.6
-1.6
-1.0
N.D.
-1.7
nitrogen source
DL-2-Aminobutyric (N)
-2.1
-1.4
-1.9
N.D.
-1.1
nitrogen source
Caprolactam (N)
-1.5
-1.6
-1.0
N.D.
-2.4
nitrogen source
nitrite (N)
-2.6
-1.5
-1.1
N.D.
-0.9
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
-0.0
-5.2
N.D.
-0.9
nitrogen source
nitrite (N)
-2.8
-1.5
-0.9
N.D.
-0.7
nitrogen source
1,6-Hexanediamine (N)
-2.3
-1.3
-1.1
N.D.
-1.1
stress
R2A with Fusaric 240 ug/mL
-0.2
-0.2
-5.2
N.D.
-0.3
nitrogen source
1,6-Hexanediamine (N)
-2.2
-1.3
-0.8
N.D.
-1.5
nitrogen source
Uracil (N)
-1.9
-1.2
-1.4
N.D.
-1.1
nitrogen source
Uracil (N)
-2.4
-1.2
-0.7
N.D.
-1.1
nitrogen source
Propiolactam (N)
-1.3
-1.1
-1.3
N.D.
-1.5
carbon source
gluconate (C)
-2.5
-1.2
-0.9
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-2.1
+0.2
-1.8
N.D.
-1.4
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-4.7
+0.1
-0.2
N.D.
+0.3
carbon source
carbon source 4-vinylphenol 0.01 mM
-4.8
+0.0
+0.4
N.D.
+0.0
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-4.5
+0.2
+0.4
N.D.
-0.3
pH
pH 5
-3.7
-0.1
+0.6
N.D.
-1.0
carbon source
carbon source Glycerol 108.6 mM
-5.0
-0.1
+0.9
N.D.
-0.1
carbon source
carbon source Bisabolol 45 mM
-4.2
-0.2
+1.2
N.D.
-0.2
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-3.7
+0.2
-0.4
N.D.
+0.7
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
-3.2
+0.0
+1.3
N.D.
-1.3
pH
pH 6
-4.5
-0.2
+1.2
N.D.
+0.5
pH
pH 8
-3.9
+0.1
+0.8
N.D.
+0.3
pH
pH 4
-3.7
-0.1
+1.2
N.D.
+0.1
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