Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3348 and PP_3349 are separated by 15 nucleotidesPP_3349 and PP_3350 are separated by 34 nucleotidesPP_3350 and PP_3352 are separated by 261 nucleotidesPP_3352 and PP_3353 overlap by 4 nucleotides PP_3348: PP_3348 - GGDEF domain protein, at 3,784,806 to 3,785,951 _3348 PP_3349: PP_3349 - 3-hydroxyphenylpropionate / 3-hydroxycinnamate:H+ symporter, at 3,785,967 to 3,787,208 _3349 PP_3350: PP_3350 - conserved exported protein of unknown function, at 3,787,243 to 3,788,634 _3350 PP_3352: PP_3352 - Arylsulfatase, at 3,788,896 to 3,790,560 _3352 PP_3353: PP_3353 - putative sulfatase-modifying factor (C-alpha-formyglycine- generating enzyme), at 3,790,557 to 3,791,582 _3353
Group Condition PP_3348 PP_3349 PP_3350 PP_3352 PP_3353
phage P. putida Sci Big MOI 0.1 -0.9 -0.3 -0.1 -0.8 -1.6
phage P. putida Sci small MOI 0.1 -0.3 -0.9 -0.0 -0.2 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 +0.0 +0.3 -0.2 -1.8
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.3 -0.3 -0.1 -0.6 -0.2
nitrogen source 2,6-diaminopimelic (N) -0.8 -0.6 -0.1 -0.6 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.5 -0.5 -0.6 +0.2 -0.2
stress R2A with Fusaric 120 ug/mL -0.5 -0.5 +0.2 -0.1 -0.5
phage P. putida PUT 7 MOI 1 -0.6 -0.6 -0.9 +0.1 +0.6
pH Valerolactone (C) pH 6.5 +0.1 -0.1 -0.5 -0.0 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 -0.1 -0.6 -0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.3 -0.7 +0.5 -0.2
phage P. putida Sci small MOI 0.1 +0.1 -0.7 +0.3 -0.4 -0.4
carbon source carbon source 3-O-methyl-glucose 10 mM -0.6 -0.2 +0.4 -0.2 -0.3
temperature shift Temperature shift 30_to_34; with MOPS -1.1 -0.3 +0.2 +0.1 +0.2
carbon source Tween 20 (C) -0.1 +0.2 -0.4 +0.4 -0.6
phage P. put South MOI 10 +0.0 -1.0 +0.2 -0.2 +0.5
stress Growth with Chloride 500 mM; with MOPS -0.2 -0.5 +0.1 -0.1 +0.7
pH pH 7 -0.5 -0.1 +0.3 -0.3 +0.6
temperature shift Temperature shift 30_to_21; with MOPS +0.2 -0.6 -0.2 +0.1 +0.7
pH Growth at pH6 and (C) D-Glucose -0.4 -0.4 -0.0 +0.1 +1.2
temperature Growth at 25C; with MOPS +0.3 -0.4 -0.0 -0.0 +0.9
temperature Growth at 30C; with MOPS +0.2 -0.4 +0.4 +0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.3 +0.5 +0.2 -0.4
nitrogen source Urea (N) +0.4 +0.3 +0.1 -0.3 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 +0.3 -0.0 +0.3 -0.2
nitrogen source nitrogen source nitrite 0.5mM +0.4 +0.5 +0.2 -0.0 +0.5
carbon source ferulate (C); with MOPS -0.4 -0.1 +0.7 +0.3 +1.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.0 -0.1 +0.0 +1.5
carbon source ferulate (C); with MOPS -0.2 +0.1 +1.0 +0.5 +0.2
nitrogen source Ammonium chloride and TNT (N) +0.2 +0.4 +0.5 +0.2 +0.6
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