Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3346 and PP_3348 are separated by 307 nucleotidesPP_3348 and PP_3349 are separated by 15 nucleotidesPP_3349 and PP_3350 are separated by 34 nucleotidesPP_3350 and PP_3352 are separated by 261 nucleotides PP_3346: PP_3346 - Nickel transporter ATP-binding protein, at 3,783,677 to 3,784,498 _3346 PP_3348: PP_3348 - GGDEF domain protein, at 3,784,806 to 3,785,951 _3348 PP_3349: PP_3349 - 3-hydroxyphenylpropionate / 3-hydroxycinnamate:H+ symporter, at 3,785,967 to 3,787,208 _3349 PP_3350: PP_3350 - conserved exported protein of unknown function, at 3,787,243 to 3,788,634 _3350 PP_3352: PP_3352 - Arylsulfatase, at 3,788,896 to 3,790,560 _3352
Group Condition PP_3346 PP_3348 PP_3349 PP_3350 PP_3352
phage P. putida Sci small MOI 0.1 -5.3 +0.1 -0.7 +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -3.3 -0.1 +0.0 -0.1 -0.3
phage P. putida Sci Big MOI 0.1 -1.1 -0.9 -0.3 -0.1 -0.8
phage P. putida Sci small MOI 0.1 -1.3 -0.3 -0.9 -0.0 -0.2
pH Growth at pH6 and (C) D-Glucose -2.0 -0.4 -0.4 -0.0 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.9 -0.1 -0.4 -0.1 +0.1
nitrogen source 2,6-diaminopimelic (N) -0.4 -0.8 -0.6 -0.1 -0.6
temperature shift Temperature shift 30_to_25; with MOPS -1.4 -0.1 -0.4 -0.3 +0.0
micoeukaryotes Acanthamoeba sp., muxed culture-2 -1.7 -0.3 -0.1 -0.1 -0.0
phage P. put South MOI 10 -1.1 +0.0 -1.0 +0.2 -0.2
carbon source 1,3-Butandiol (C) -1.1 -0.3 -0.5 -0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -2.1 -0.1 +0.2 -0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.6 -0.2 -0.0 -0.3 +0.1
phage P. putida PUT 7 MOI 1 -0.0 -0.6 -0.6 -0.9 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.5 -0.3 -0.7 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.2 +0.2 -0.4 -0.5 -0.0
pH Valerolactone (C) pH 6.5 -1.4 +0.1 -0.1 -0.5 -0.0
carbon source BC Lignin Sorghum 10 vol% (C) -1.0 -0.2 -0.1 -0.6 +0.1
r2a control with 0.2% methanol R2A control with 0.2% methanol -1.8 -0.1 +0.1 -0.0 -0.0
temperature shift Temperature shift 30_to_34; with MOPS -0.6 -1.1 -0.3 +0.2 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -1.1 +0.3 -0.5 +0.1 -0.3
carbon source Tween 20 (C) -1.1 -0.1 +0.2 -0.4 +0.4
carbon source ferulate (C); with MOPS -2.3 -0.2 +0.1 +1.0 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.8 -0.5 -0.5 -0.6 +0.2
carbon source 3-methyl-1-butanol 0.25 vol% (C) -1.1 +0.1 +0.4 -0.3 +0.3
nitrogen source NAG (N); with MOPS -0.7 +0.7 +0.2 -0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 +1.0 +0.3 -0.0 +0.3
stress R2A with Polymyxin B sulfate 0.001 mg/ml +1.1 -0.4 +0.3 -0.2 +0.0
micoeukaryotes Tetrahymena sp., Mixed culture-2 +2.0 -0.0 +0.0 +0.0 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +2.1 +0.3 -0.3 +0.0 -0.2
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