Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3345 and PP_3346 overlap by 4 nucleotidesPP_3346 and PP_3348 are separated by 307 nucleotidesPP_3348 and PP_3349 are separated by 15 nucleotidesPP_3349 and PP_3350 are separated by 34 nucleotides PP_3345: PP_3345 - nickel ABC transporter ATP-binding subunit, at 3,782,910 to 3,783,680 _3345 PP_3346: PP_3346 - Nickel transporter ATP-binding protein, at 3,783,677 to 3,784,498 _3346 PP_3348: PP_3348 - GGDEF domain protein, at 3,784,806 to 3,785,951 _3348 PP_3349: PP_3349 - 3-hydroxyphenylpropionate / 3-hydroxycinnamate:H+ symporter, at 3,785,967 to 3,787,208 _3349 PP_3350: PP_3350 - conserved exported protein of unknown function, at 3,787,243 to 3,788,634 _3350
Group Condition PP_3345 PP_3346 PP_3348 PP_3349 PP_3350
phage P. putida Sci small MOI 0.1 N.D. -5.3 +0.1 -0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -3.3 -0.1 +0.0 -0.1
pH Growth at pH6 and (C) D-Glucose N.D. -2.0 -0.4 -0.4 -0.0
phage P. putida Sci small MOI 0.1 N.D. -1.3 -0.3 -0.9 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -1.9 -0.1 -0.4 -0.1
phage P. putida Sci Big MOI 0.1 N.D. -1.1 -0.9 -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.9 -0.5 -0.3 -0.7
temperature shift Temperature shift 30_to_25; with MOPS N.D. -1.4 -0.1 -0.4 -0.3
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -1.7 -0.3 -0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.6 -0.2 -0.0 -0.3
phage P. putida PUT 7 MOI 1 N.D. -0.0 -0.6 -0.6 -0.9
carbon source 1,3-Butandiol (C) N.D. -1.1 -0.3 -0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -2.1 -0.1 +0.2 -0.1
carbon source BC Lignin Sorghum 10 vol% (C) N.D. -1.0 -0.2 -0.1 -0.6
phage P. put South MOI 10 N.D. -1.1 +0.0 -1.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -1.2 +0.2 -0.4 -0.5
pH Valerolactone (C) pH 6.5 N.D. -1.4 +0.1 -0.1 -0.5
r2a control with 0.2% methanol R2A control with 0.2% methanol N.D. -1.8 -0.1 +0.1 -0.0
temperature shift Temperature shift 30_to_34; with MOPS N.D. -0.6 -1.1 -0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.3 +0.1 -0.3 -0.2
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. -1.0 -0.5 +0.2 -0.3
carbon source ferulate (C); with MOPS N.D. -2.3 -0.2 +0.1 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. -1.1 +0.3 -0.5 +0.1
carbon source 3-methyl-1-butanol 0.25 vol% (C) N.D. -1.1 +0.1 +0.4 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.8 -0.5 -0.5 -0.6
nitrogen source NAG (N); with MOPS N.D. -0.7 +0.7 +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.3 +1.0 +0.3 -0.0
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. +1.1 -0.4 +0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +2.1 +0.3 -0.3 +0.0
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. +2.0 -0.0 +0.0 +0.0
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