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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3345 and PP_3346 overlap by 4 nucleotides
PP_3346 and PP_3348 are separated by 307 nucleotides
PP_3348 and PP_3349 are separated by 15 nucleotides
PP_3349 and PP_3350 are separated by 34 nucleotides
PP_3345: PP_3345 - nickel ABC transporter ATP-binding subunit, at 3,782,910 to 3,783,680
_3345
PP_3346: PP_3346 - Nickel transporter ATP-binding protein, at 3,783,677 to 3,784,498
_3346
PP_3348: PP_3348 - GGDEF domain protein, at 3,784,806 to 3,785,951
_3348
PP_3349: PP_3349 - 3-hydroxyphenylpropionate / 3-hydroxycinnamate:H+ symporter, at 3,785,967 to 3,787,208
_3349
PP_3350: PP_3350 - conserved exported protein of unknown function, at 3,787,243 to 3,788,634
_3350
Group
Condition
PP
_3345
PP
_3346
PP
_3348
PP
_3349
PP
_3350
phage
P. putida Sci small MOI 0.1
N.D.
-5.3
+0.1
-0.7
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
N.D.
-3.3
-0.1
+0.0
-0.1
pH
Growth at pH6 and (C) D-Glucose
N.D.
-2.0
-0.4
-0.4
-0.0
phage
P. putida Sci small MOI 0.1
N.D.
-1.3
-0.3
-0.9
-0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
-1.9
-0.1
-0.4
-0.1
phage
P. putida Sci Big MOI 0.1
N.D.
-1.1
-0.9
-0.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.9
-0.5
-0.3
-0.7
temperature shift
Temperature shift 30_to_25; with MOPS
N.D.
-1.4
-0.1
-0.4
-0.3
micoeukaryotes
Acanthamoeba sp., muxed culture-2
N.D.
-1.7
-0.3
-0.1
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.6
-0.2
-0.0
-0.3
phage
P. putida PUT 7 MOI 1
N.D.
-0.0
-0.6
-0.6
-0.9
carbon source
1,3-Butandiol (C)
N.D.
-1.1
-0.3
-0.5
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
-2.1
-0.1
+0.2
-0.1
carbon source
BC Lignin Sorghum 10 vol% (C)
N.D.
-1.0
-0.2
-0.1
-0.6
phage
P. put South MOI 10
N.D.
-1.1
+0.0
-1.0
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
-1.2
+0.2
-0.4
-0.5
pH
Valerolactone (C) pH 6.5
N.D.
-1.4
+0.1
-0.1
-0.5
r2a control with 0.2% methanol
R2A control with 0.2% methanol
N.D.
-1.8
-0.1
+0.1
-0.0
temperature shift
Temperature shift 30_to_34; with MOPS
N.D.
-0.6
-1.1
-0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.3
+0.1
-0.3
-0.2
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
N.D.
-1.0
-0.5
+0.2
-0.3
carbon source
ferulate (C); with MOPS
N.D.
-2.3
-0.2
+0.1
+1.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
N.D.
-1.1
+0.3
-0.5
+0.1
carbon source
3-methyl-1-butanol 0.25 vol% (C)
N.D.
-1.1
+0.1
+0.4
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
N.D.
+0.8
-0.5
-0.5
-0.6
nitrogen source
NAG (N); with MOPS
N.D.
-0.7
+0.7
+0.2
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.3
+1.0
+0.3
-0.0
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
N.D.
+1.1
-0.4
+0.3
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+2.1
+0.3
-0.3
+0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-2
N.D.
+2.0
-0.0
+0.0
+0.0
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