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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3316 and PP_3317 are separated by 53 nucleotides
PP_3317 and PP_3318 overlap by 14 nucleotides
PP_3318 and PP_3319 are separated by 269 nucleotides
PP_3319 and PP_3320 are separated by 185 nucleotides
PP_3316: PP_3316 - putative Chaperone-associated ATPase, at 3,751,060 to 3,753,882
_3316
PP_3317: PP_3317 - conserved protein of unknown function, at 3,753,936 to 3,754,061
_3317
PP_3318: PP_3318 - conserved protein of unknown function, at 3,754,048 to 3,754,290
_3318
PP_3319: PP_3319 - Sensory box protein/GGDEF domain protein, at 3,754,560 to 3,756,761
_3319
PP_3320: PP_3320 - conserved protein of unknown function, at 3,756,947 to 3,757,114
_3320
Group
Condition
PP
_3316
PP
_3317
PP
_3318
PP
_3319
PP
_3320
phage
P. putida Missley MOI 0.1
+0.3
N.D.
-4.4
-0.2
-0.1
nitrogen source
NAG (N); with MOPS
-0.4
N.D.
-2.7
+0.0
-0.3
temperature shift
Temperature shift 30_to_10; with MOPS
-0.1
N.D.
-2.5
+0.1
-0.8
nitrogen source
D-2-Aminobutyric (N)
+0.0
N.D.
-1.1
-0.1
-1.8
phage
P. putida Sci Big MOI 0.1
-0.5
N.D.
-1.8
-0.0
-0.5
stress
R2A with Fusaric 240 ug/mL
-0.0
N.D.
-2.3
-0.1
-0.2
pH
Growth at pH7 and (C) Trisodium citrate
-0.1
N.D.
-1.2
-0.1
-1.0
phage
P. putida PUT10 MOI 10
+0.1
N.D.
-1.0
-0.3
-1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
N.D.
-1.2
+0.1
-1.0
stress
Growth with Chloride 200 mM; with MOPS
-0.0
N.D.
-2.5
+0.3
+0.2
stress
Growth with Chloride 500 mM; with MOPS
+0.0
N.D.
-2.0
+0.1
-0.2
carbon source
L-Leucine (C)
-0.0
N.D.
-1.2
+0.1
-0.9
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.3
N.D.
-1.1
+0.1
-0.6
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.3
N.D.
-1.5
-0.2
+0.1
pH
pH 9
+0.3
N.D.
-1.8
-0.2
-0.0
temperature
Growth at 25C
-0.1
N.D.
-1.9
-0.3
+0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.1
N.D.
-0.7
+0.3
-1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
N.D.
-1.6
+0.1
+0.4
pH
Growth at pH6 and (C) Trisodium citrate
+0.2
N.D.
-1.5
+0.1
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.2
N.D.
-1.3
+0.2
+1.2
pH
Growth at pH8 and (C) D-Glucose
-0.0
N.D.
+0.9
+0.3
-1.0
carbon source
Valerolactone (C)
-0.2
N.D.
-1.1
+0.0
+1.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
N.D.
-1.3
+0.2
+0.8
phage
PUT 8 MOI 1
+0.1
N.D.
+1.6
+0.1
-1.6
pH
Growth at pH7 and (C) D-Glucose
-0.2
N.D.
+1.7
+0.2
-0.4
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.2
N.D.
+0.7
-0.1
+1.1
nitrogen source
2,6-diaminopimelic (N)
-0.4
N.D.
+1.7
+0.0
+0.5
pH
pH 8
+0.1
N.D.
+1.0
-0.1
+1.0
micoeukaryotes
Tetrahymena sp., Mixed culture-2
+0.1
N.D.
+1.6
+0.2
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.3
N.D.
+1.3
+0.1
+0.6
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