Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3316 and PP_3317 are separated by 53 nucleotidesPP_3317 and PP_3318 overlap by 14 nucleotidesPP_3318 and PP_3319 are separated by 269 nucleotidesPP_3319 and PP_3320 are separated by 185 nucleotides PP_3316: PP_3316 - putative Chaperone-associated ATPase, at 3,751,060 to 3,753,882 _3316 PP_3317: PP_3317 - conserved protein of unknown function, at 3,753,936 to 3,754,061 _3317 PP_3318: PP_3318 - conserved protein of unknown function, at 3,754,048 to 3,754,290 _3318 PP_3319: PP_3319 - Sensory box protein/GGDEF domain protein, at 3,754,560 to 3,756,761 _3319 PP_3320: PP_3320 - conserved protein of unknown function, at 3,756,947 to 3,757,114 _3320
Group Condition PP_3316 PP_3317 PP_3318 PP_3319 PP_3320
phage P. putida Missley MOI 0.1 +0.3 N.D. -4.4 -0.2 -0.1
nitrogen source NAG (N); with MOPS -0.4 N.D. -2.7 +0.0 -0.3
temperature shift Temperature shift 30_to_10; with MOPS -0.1 N.D. -2.5 +0.1 -0.8
nitrogen source D-2-Aminobutyric (N) +0.0 N.D. -1.1 -0.1 -1.8
phage P. putida Sci Big MOI 0.1 -0.5 N.D. -1.8 -0.0 -0.5
stress R2A with Fusaric 240 ug/mL -0.0 N.D. -2.3 -0.1 -0.2
pH Growth at pH7 and (C) Trisodium citrate -0.1 N.D. -1.2 -0.1 -1.0
phage P. putida PUT10 MOI 10 +0.1 N.D. -1.0 -0.3 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -1.2 +0.1 -1.0
stress Growth with Chloride 200 mM; with MOPS -0.0 N.D. -2.5 +0.3 +0.2
stress Growth with Chloride 500 mM; with MOPS +0.0 N.D. -2.0 +0.1 -0.2
carbon source L-Leucine (C) -0.0 N.D. -1.2 +0.1 -0.9
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 N.D. -1.1 +0.1 -0.6
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.3 N.D. -1.5 -0.2 +0.1
pH pH 9 +0.3 N.D. -1.8 -0.2 -0.0
temperature Growth at 25C -0.1 N.D. -1.9 -0.3 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.7 +0.3 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. -1.6 +0.1 +0.4
pH Growth at pH6 and (C) Trisodium citrate +0.2 N.D. -1.5 +0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 N.D. -1.3 +0.2 +1.2
pH Growth at pH8 and (C) D-Glucose -0.0 N.D. +0.9 +0.3 -1.0
carbon source Valerolactone (C) -0.2 N.D. -1.1 +0.0 +1.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. -1.3 +0.2 +0.8
phage PUT 8 MOI 1 +0.1 N.D. +1.6 +0.1 -1.6
pH Growth at pH7 and (C) D-Glucose -0.2 N.D. +1.7 +0.2 -0.4
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 N.D. +0.7 -0.1 +1.1
nitrogen source 2,6-diaminopimelic (N) -0.4 N.D. +1.7 +0.0 +0.5
pH pH 8 +0.1 N.D. +1.0 -0.1 +1.0
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.1 N.D. +1.6 +0.2 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.3 N.D. +1.3 +0.1 +0.6
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