Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3304 and PP_3305 are separated by 152 nucleotidesPP_3305 and PP_3306 are separated by 412 nucleotidesPP_3306 and PP_3307 are separated by 149 nucleotidesPP_3307 and PP_3308 are separated by 111 nucleotides PP_3304: PP_3304 - Multidrug resistance transporter, Bcr/CflA family, at 3,739,558 to 3,740,796 _3304 PP_3305: PP_3305 - Membrane protein, TerC family, at 3,740,949 to 3,741,932 _3305 PP_3306: PP_3306 - conserved protein of unknown function, at 3,742,345 to 3,742,551 _3306 PP_3307: PP_3307 - conserved protein of unknown function, at 3,742,701 to 3,743,081 _3307 PP_3308: PP_3308 - promiscuous aromatic aldehyde dehydrogenase, 2Fe-2S subunit, at 3,743,193 to 3,743,720 _3308
Group Condition PP_3304 PP_3305 PP_3306 PP_3307 PP_3308
phage P. putida Missley MOI 0.1 -0.1 +0.2 N.D. -4.4 -0.4
temperature shift Temperature shift 10_to_25; with MOPS +0.5 -0.1 N.D. -4.8 +0.1
nitrogen source Ammonium chloride (N) -0.1 +0.0 N.D. -3.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 +0.1 N.D. -3.0 -0.2
carbon source 5-Aminovaleric 10 mM (C) +0.0 -0.5 N.D. -1.9 -0.2
carbon source ferulate (C); with MOPS +0.8 -0.2 N.D. -3.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 -0.1 N.D. -1.6 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.0 +0.3 N.D. -2.0 -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.0 N.D. -1.5 -0.3
carbon source carbon source D-Glucose 55.5 mM -0.4 +0.6 N.D. -2.0 +0.0
phage PUT 8 MOI 1 -0.0 +0.2 N.D. -1.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.0 N.D. -1.3 -0.3
phage P. putida Sci small MOI 0.1 -0.1 +0.3 N.D. -0.8 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.2 -0.4 N.D. -1.2 +0.2
phage P. putida Sci Big MOI 0.1 -0.4 -0.3 N.D. +0.4 -0.7
nitrogen source D-2-Aminobutyric (N) -0.2 +0.3 N.D. -1.2 +0.2
phage P. putida Sci small MOI 0.1 +0.4 -0.1 N.D. -1.3 +0.1
stress Growth with Chloride 200 mM; with MOPS +0.3 +0.5 N.D. -1.6 -0.0
carbon source D-Glucose (C) +0.2 +0.2 N.D. -1.4 +0.1
carbon source ferulate (C); with MOPS +0.3 +0.5 N.D. -1.2 -0.4
temperature Growth at 30C; with MOPS +0.1 +0.3 N.D. -1.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 +0.2 N.D. -1.2 +0.3
pH pH 9 +0.5 +0.6 N.D. -0.5 -0.3
nitrogen source Ammonium chloride and TNT (N) -0.0 -0.3 N.D. +1.4 -0.1
pH Growth at pH7 and (C) Trisodium citrate +0.4 +0.0 N.D. +1.2 -0.5
carbon source acetate (C) +0.7 +0.1 N.D. +1.0 -0.6
carbon source D-Glucose (C) +0.4 -0.2 N.D. +1.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.1 +0.4 N.D. +1.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.2 N.D. +2.1 +0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 +0.4 N.D. +6.5 +0.1
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