Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3291 and PP_3292 are separated by 72 nucleotidesPP_3292 and PP_3293 are separated by 59 nucleotidesPP_3293 and PP_3294 are separated by 212 nucleotidesPP_3294 and PP_3295 are separated by 10 nucleotides PP_3291: PP_3291 - Metallo-beta-lactamase family protein, at 3,725,348 to 3,726,715 _3291 PP_3292: PP_3292 - conserved membrane protein of unknown function, at 3,726,788 to 3,727,432 _3292 PP_3293: PP_3293 - conserved membrane protein of unknown function, at 3,727,492 to 3,727,917 _3293 PP_3294: PP_3294 - Universal stress protein family, at 3,728,130 to 3,729,020 _3294 PP_3295: PP_3295 - YeeE/YedE family protein, at 3,729,031 to 3,729,510 _3295
Group Condition PP_3291 PP_3292 PP_3293 PP_3294 PP_3295
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.0 -0.4 -0.2 -0.8 -1.1
temperature Growth at 25C -0.2 -0.0 -0.0 -0.8 -1.2
phage P. putida Missley MOI 0.1 -0.3 +0.1 -0.7 -0.2 -1.0
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.1 -0.4 -0.3 -0.7 -0.6
phage P. putida Sci Big MOI 0.1 -0.1 -0.3 -0.9 -0.7 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.3 +0.1 +0.1 -0.0 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -0.3 -0.6 -1.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.1 -0.1 +0.1 +0.2 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 -0.1 +0.3 -0.1 -1.2
nitrogen source 2,6-diaminopimelic (N) +0.2 -1.6 -0.7 +0.4 +0.4
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.1 -0.3 +0.4 -0.6 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 +0.4 -0.0 -0.4 -1.2
pH Valerolactone (C) pH 6.5 +0.0 -0.9 +0.1 +0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -0.3 +0.6 -1.0 -0.5
carbon source Tween 20 (C) -0.3 -1.0 +0.4 -0.3 +0.2
pH Growth at pH8 and (C) Trisodium citrate -0.1 -0.4 -0.1 -0.9 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 +0.1 +0.4 -0.7 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.0 +0.4 +0.3 +0.0 -1.4
carbon source D-Glucose (C); with MOPS -0.4 -0.5 +0.0 -0.3 +0.6
stress Growth with Chloride 500 mM; with MOPS -0.2 -0.3 +0.4 -0.7 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +0.9 -0.1 -0.5 -0.3
carbon source ferulate (C); with MOPS -0.1 +0.3 +0.3 -0.9 +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.2 +0.6 -0.6 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -1.0 -0.1 +1.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.1 +0.5 +0.8 -0.6
nitrogen source Propandiamine (N) -0.0 +0.4 -0.2 -0.3 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.1 +0.2 +1.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +1.3 +0.1 +0.1 -0.3
nitrogen source 2,6-diaminopimelic (N) -0.2 +0.6 -0.2 +0.7 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.2 +0.1 +0.2 +1.4
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