Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5597 and PP_3270 are separated by 251 nucleotidesPP_3270 and PP_3271 are separated by 37 nucleotidesPP_3271 and PP_3272 are separated by 18 nucleotidesPP_3272 and PP_3273 overlap by 4 nucleotides PP_5597: PP_5597 - conserved protein of unknown function, at 3,703,525 to 3,703,695 _5597 PP_3270: PP_3270 - oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, at 3,703,947 to 3,706,001 _3270 PP_3271: PP_3271 - phenylacetic acid-specific porin, at 3,706,039 to 3,707,292 _3271 PP_3272: PP_3272 - acetate permease, at 3,707,311 to 3,708,873 _3272 PP_3273: PP_3273 - conserved protein of unknown function, at 3,708,870 to 3,709,178 _3273
Group Condition PP_5597 PP_3270 PP_3271 PP_3272 PP_3273
carbon source Phenylacetic (C) -0.1 -6.2 -2.1 -6.5 -3.8
carbon source Phenylacetic (C) -0.1 -4.8 -1.8 -3.5 -3.5
phage P. putida Sci Big MOI 0.1 -5.5 +0.1 -0.1 -0.3 +0.0
phage P. putida PUT 7 MOI 1 -1.2 -0.5 -0.4 -0.2 -1.0
phage P. putida Missley MOI 0.1 -1.2 -0.1 -0.7 -0.4 -0.1
phage P. putida Sci small MOI 0.1 -1.0 -0.1 -0.9 -0.1 -0.2
carbon source L-Phenylalanine 5 mM (C) -1.7 -0.0 -0.1 +0.0 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.0 -0.2 -0.1 -0.2 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.2 -0.2 -0.1 -0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.3 -0.1 -0.1 -0.1 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.9 +0.0 +0.1 -0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.0 -0.2 +0.1 -0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -1.4 +0.2 -0.2 -0.2 +0.1
phage P. put South MOI 10 -1.3 +0.2 -0.1 -0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.5 +0.1 -0.3 +0.1 +0.2
stress Growth with Chloride 500 mM; with MOPS -1.3 -0.2 +0.0 -0.1 +0.2
pH Growth at pH9 and (C) D-Glucose -0.8 -0.2 -0.3 -0.2 +0.5
pH Growth at pH6 and (C) Trisodium citrate -1.3 +0.1 -0.2 -0.2 +0.6
carbon source Myristic (C) +0.5 +0.1 +0.1 -0.2 -0.8
stress R2A with Fusaric 240 ug/mL +0.7 -0.4 -0.1 -0.1 -0.4
pH Growth at pH6 and (C) D-Glucose -0.8 -0.1 +0.2 +0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 -0.1 -0.2 +0.0 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +0.1 -0.3 +0.3 -0.5
temperature Growth at 25C +0.9 -0.4 +0.2 +0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +0.6 -0.0 +0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.0 -0.2 -0.1 +0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.2 -0.4 -0.1 +0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.3 -0.1 +0.1 -0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.4 +0.1 +0.3 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.5 +0.3 +0.1 +0.0 -0.1
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