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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5597 and PP_3270 are separated by 251 nucleotides
PP_3270 and PP_3271 are separated by 37 nucleotides
PP_3271 and PP_3272 are separated by 18 nucleotides
PP_3272 and PP_3273 overlap by 4 nucleotides
PP_5597: PP_5597 - conserved protein of unknown function, at 3,703,525 to 3,703,695
_5597
PP_3270: PP_3270 - oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, at 3,703,947 to 3,706,001
_3270
PP_3271: PP_3271 - phenylacetic acid-specific porin, at 3,706,039 to 3,707,292
_3271
PP_3272: PP_3272 - acetate permease, at 3,707,311 to 3,708,873
_3272
PP_3273: PP_3273 - conserved protein of unknown function, at 3,708,870 to 3,709,178
_3273
Group
Condition
PP
_5597
PP
_3270
PP
_3271
PP
_3272
PP
_3273
carbon source
Phenylacetic (C)
-0.1
-6.2
-2.1
-6.5
-3.8
carbon source
Phenylacetic (C)
-0.1
-4.8
-1.8
-3.5
-3.5
phage
P. putida Sci Big MOI 0.1
-5.5
+0.1
-0.1
-0.3
+0.0
phage
P. putida PUT 7 MOI 1
-1.2
-0.5
-0.4
-0.2
-1.0
phage
P. putida Missley MOI 0.1
-1.2
-0.1
-0.7
-0.4
-0.1
phage
P. putida Sci small MOI 0.1
-1.0
-0.1
-0.9
-0.1
-0.2
carbon source
L-Phenylalanine 5 mM (C)
-1.7
-0.0
-0.1
+0.0
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-1.0
-0.2
-0.1
-0.2
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-1.2
-0.2
-0.1
-0.2
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-1.3
-0.1
-0.1
-0.1
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.9
+0.0
+0.1
-0.2
-0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.0
-0.2
+0.1
-0.3
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-1.4
+0.2
-0.2
-0.2
+0.1
phage
P. put South MOI 10
-1.3
+0.2
-0.1
-0.1
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.5
+0.1
-0.3
+0.1
+0.2
stress
Growth with Chloride 500 mM; with MOPS
-1.3
-0.2
+0.0
-0.1
+0.2
pH
Growth at pH9 and (C) D-Glucose
-0.8
-0.2
-0.3
-0.2
+0.5
pH
Growth at pH6 and (C) Trisodium citrate
-1.3
+0.1
-0.2
-0.2
+0.6
carbon source
Myristic (C)
+0.5
+0.1
+0.1
-0.2
-0.8
stress
R2A with Fusaric 240 ug/mL
+0.7
-0.4
-0.1
-0.1
-0.4
pH
Growth at pH6 and (C) D-Glucose
-0.8
-0.1
+0.2
+0.3
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.9
-0.1
-0.2
+0.0
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.8
+0.1
-0.3
+0.3
-0.5
temperature
Growth at 25C
+0.9
-0.4
+0.2
+0.1
-0.3
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
+0.6
-0.0
+0.1
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+1.0
-0.2
-0.1
+0.3
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+1.2
-0.4
-0.1
+0.3
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+1.3
-0.1
+0.1
-0.1
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.4
+0.1
+0.3
-0.2
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+1.5
+0.3
+0.1
+0.0
-0.1
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