Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3262 and PP_5595 are separated by 91 nucleotidesPP_5595 and PP_3263 are separated by 28 nucleotidesPP_3263 and PP_3264 overlap by 1 nucleotidesPP_3264 and PP_3265 overlap by 4 nucleotides PP_3262: PP_3262 - putative Metallothionein, at 3,696,756 to 3,696,980 _3262 PP_5595: PP_5595 - conserved exported protein of unknown function, at 3,697,072 to 3,697,488 _5595 PP_3263: PP_3263 - phospholipid modification enzyme, at 3,697,517 to 3,698,470 _3263 PP_3264: PP_3264 - cardiolipin synthase 2, at 3,698,470 to 3,699,672 _3264 PP_3265: PP_3265 - putative phosphohydrolase of unknown function, at 3,699,669 to 3,700,442 _3265
Group Condition PP_3262 PP_5595 PP_3263 PP_3264 PP_3265
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.1 -0.4 -0.7 -1.6
pH Growth at pH7 and (C) Trisodium citrate N.D. -0.7 +0.1 -0.1 -1.8
phage P. putida Missley MOI 0.1 N.D. -0.5 -0.4 -0.1 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -0.2 +0.1 -0.1 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.0 -0.2 -1.0 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -0.5 +0.1 -0.3 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.4 -0.3 -0.2 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -0.5 +0.2 -0.3 -1.0
nitrogen source NAG (N); with MOPS N.D. +0.1 -0.2 -0.3 -1.1
phage P. putida PUT 7 MOI 1 N.D. -0.9 +0.2 -0.4 -0.3
pH Growth at pH9 and (C) Trisodium citrate N.D. +0.3 -0.3 -0.2 -1.0
carbon source carbon source D-Glucose 55.5 mM N.D. +0.1 +0.0 +0.2 -1.4
carbon source Valerolactone (C) N.D. +0.0 +0.3 -0.0 -1.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.4 +0.2 +0.1 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.3 -0.2 -0.2 -0.9
nitrogen source D-2-Aminobutyric (N) N.D. -0.6 -0.1 -0.8 +0.6
pH pH 6 N.D. -0.3 +0.1 +0.4 -0.8
pH Growth at pH7 and (C) Trisodium citrate N.D. +0.5 +0.3 +0.6 -1.8
pH pH 7 N.D. +0.1 -0.4 +0.6 -0.7
carbon source D-Glucose (C) N.D. +0.2 -0.3 +0.6 -0.7
pH Growth at pH7 and (C) Trisodium citrate N.D. -0.5 +0.1 -0.3 +0.9
temperature shift Temperature shift 10_to_30; with MOPS N.D. +0.2 -0.1 -0.6 +0.8
pH Growth at pH6 and (C) D-Glucose N.D. +0.2 -0.5 -0.1 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -0.3 -0.5 +1.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -0.4 +0.3 +1.0
temperature shift Temperature shift 30_to_4 N.D. -0.5 +0.0 +0.2 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 +0.1 +0.4 +1.0
temperature shift Temperature shift 30_to_21; with MOPS N.D. -0.3 +0.4 -0.4 +1.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.6 +0.4 +0.6 -0.1
nitrogen source nitrogen source nitrite 1mM N.D. +0.6 +0.3 +0.4 +0.4
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