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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3252 and PP_3253 are separated by 6 nucleotides
PP_3253 and PP_3254 overlap by 10 nucleotides
PP_3254 and PP_3255 are separated by 85 nucleotides
PP_3255 and PP_3256 are separated by 21 nucleotides
PP_3252: PP_3252 - Modification methylase, HemK family, at 3,686,927 to 3,687,877
_3252
PP_3253: PP_3253 - Carboxylate-amine ligase PP_3253, at 3,687,884 to 3,688,990
_3253
PP_3254: PP_3254 - putative Nucleosidase, at 3,688,981 to 3,689,634
_3254
PP_3255: PP_3255 - non-homologous end joining protein, at 3,689,720 to 3,690,541
_3255
PP_3256: PP_3256 - Glycosyl transferase, group 2 family protein, at 3,690,563 to 3,691,222
_3256
Group
Condition
PP
_3252
PP
_3253
PP
_3254
PP
_3255
PP
_3256
phage
P. putida Missley MOI 0.1
-0.2
-0.2
+0.4
-0.7
-3.2
phage
P. putida Sci small MOI 0.1
+0.2
-0.7
-1.9
-0.1
-1.2
phage
P. putida PUT 7 MOI 1
+0.0
+0.1
-1.4
-0.7
-0.5
r2a control with 0.2% methanol
R2A control with 0.2% methanol
-0.3
-0.2
-0.7
-0.3
-0.4
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
+0.0
+0.1
-0.6
-0.6
-0.5
phage
P. putida Sci Big MOI 0.1
-0.3
-0.2
-0.1
-0.1
-0.7
temperature
Growth at 30C; with MOPS
-0.2
-0.5
-0.2
-0.2
-0.3
pH
Growth at pH7 and (C) Trisodium citrate
+0.2
+0.2
-0.1
-1.0
-0.6
carbon source
Crotonic (C)
-0.1
-0.6
-0.1
-0.5
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.2
+0.3
-0.2
-0.5
-0.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
+0.4
-0.6
+0.0
-0.6
pH
Growth at pH6 and (C) Trisodium citrate
+0.3
-0.2
+0.1
-0.4
-0.6
pH
Growth at pH7 and (C) D-Glucose
-0.2
+0.2
-0.6
+0.3
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+0.2
-0.1
+0.4
-0.4
-0.5
nitrogen source
Hydantoin (N)
+0.0
+0.4
-0.6
+0.2
-0.4
pH
Growth at pH6 and (C) Trisodium citrate
+0.4
+0.1
-1.0
-0.1
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
-0.2
+0.8
-0.5
-0.2
carbon source
3-Methyl-2-Oxobutanoic Acid (C)
-0.0
-0.0
+0.7
-0.2
-0.5
pH
Growth at pH8 and (C) Trisodium citrate
+0.1
+0.3
-0.4
-0.2
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.3
-0.1
+0.3
-0.3
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.5
+0.1
+0.1
+0.4
carbon source
carbon source 4-vinylphenol 0.1 mM
-0.2
-0.3
+0.5
+0.4
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.0
-0.4
+0.5
-0.0
+0.7
pH
pH 8
+0.0
-0.1
+0.2
-0.3
+1.0
pH
pH 4
-0.1
-0.3
+0.0
+0.2
+1.0
pH
Valerolactone (C) pH 6.5
-0.2
+0.4
+0.6
+0.5
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.5
+0.8
-0.0
-0.1
nitrogen source
Ammonium chloride (N)
+0.2
+0.2
+0.6
-0.1
+0.3
pH
pH 9
-0.0
+0.0
+0.3
+0.6
+0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
+0.0
+0.8
+0.5
+0.4
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