Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3252 and PP_3253 are separated by 6 nucleotidesPP_3253 and PP_3254 overlap by 10 nucleotidesPP_3254 and PP_3255 are separated by 85 nucleotidesPP_3255 and PP_3256 are separated by 21 nucleotides PP_3252: PP_3252 - Modification methylase, HemK family, at 3,686,927 to 3,687,877 _3252 PP_3253: PP_3253 - Carboxylate-amine ligase PP_3253, at 3,687,884 to 3,688,990 _3253 PP_3254: PP_3254 - putative Nucleosidase, at 3,688,981 to 3,689,634 _3254 PP_3255: PP_3255 - non-homologous end joining protein, at 3,689,720 to 3,690,541 _3255 PP_3256: PP_3256 - Glycosyl transferase, group 2 family protein, at 3,690,563 to 3,691,222 _3256
Group Condition PP_3252 PP_3253 PP_3254 PP_3255 PP_3256
phage P. putida Missley MOI 0.1 -0.2 -0.2 +0.4 -0.7 -3.2
phage P. putida Sci small MOI 0.1 +0.2 -0.7 -1.9 -0.1 -1.2
phage P. putida PUT 7 MOI 1 +0.0 +0.1 -1.4 -0.7 -0.5
r2a control with 0.2% methanol R2A control with 0.2% methanol -0.3 -0.2 -0.7 -0.3 -0.4
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.0 +0.1 -0.6 -0.6 -0.5
phage P. putida Sci Big MOI 0.1 -0.3 -0.2 -0.1 -0.1 -0.7
temperature Growth at 30C; with MOPS -0.2 -0.5 -0.2 -0.2 -0.3
pH Growth at pH7 and (C) Trisodium citrate +0.2 +0.2 -0.1 -1.0 -0.6
carbon source Crotonic (C) -0.1 -0.6 -0.1 -0.5 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.2 +0.3 -0.2 -0.5 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 +0.4 -0.6 +0.0 -0.6
pH Growth at pH6 and (C) Trisodium citrate +0.3 -0.2 +0.1 -0.4 -0.6
pH Growth at pH7 and (C) D-Glucose -0.2 +0.2 -0.6 +0.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.2 -0.1 +0.4 -0.4 -0.5
nitrogen source Hydantoin (N) +0.0 +0.4 -0.6 +0.2 -0.4
pH Growth at pH6 and (C) Trisodium citrate +0.4 +0.1 -1.0 -0.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 -0.2 +0.8 -0.5 -0.2
carbon source 3-Methyl-2-Oxobutanoic Acid (C) -0.0 -0.0 +0.7 -0.2 -0.5
pH Growth at pH8 and (C) Trisodium citrate +0.1 +0.3 -0.4 -0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -0.1 +0.3 -0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.5 +0.1 +0.1 +0.4
carbon source carbon source 4-vinylphenol 0.1 mM -0.2 -0.3 +0.5 +0.4 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.4 +0.5 -0.0 +0.7
pH pH 8 +0.0 -0.1 +0.2 -0.3 +1.0
pH pH 4 -0.1 -0.3 +0.0 +0.2 +1.0
pH Valerolactone (C) pH 6.5 -0.2 +0.4 +0.6 +0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.5 +0.8 -0.0 -0.1
nitrogen source Ammonium chloride (N) +0.2 +0.2 +0.6 -0.1 +0.3
pH pH 9 -0.0 +0.0 +0.3 +0.6 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.0 +0.8 +0.5 +0.4
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