Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3246 and PP_3247 are separated by 43 nucleotidesPP_3247 and PP_3248 are separated by 184 nucleotidesPP_3248 and PP_3249 are separated by 89 nucleotidesPP_3249 and PP_3250 are separated by 4 nucleotides PP_3246: PP_3246 - Beta-carotene hydroxylase, at 3,680,247 to 3,680,714 _3246 PP_3247: PP_3247 - Transporter, bile acid/Na+ symporter family, at 3,680,758 to 3,681,651 _3247 PP_3248: PP_3248 - Dyp-type peroxidase family protein, at 3,681,836 to 3,682,699 _3248 PP_3249: PP_3249 - putative oxidoreductase, at 3,682,789 to 3,683,700 _3249 PP_3250: PP_3250 - putative Transporter, at 3,683,705 to 3,684,988 _3250
Group Condition PP_3246 PP_3247 PP_3248 PP_3249 PP_3250
phage P. putida Missley MOI 0.1 -0.1 -3.3 -0.6 -0.9 -0.1
carbon source 3-methyl-1-butanol (C) -0.1 -0.1 -3.9 -0.3 -0.1
carbon source 3-methyl-1-butanol (C) +0.3 -0.1 -4.1 -0.2 -0.1
nitrogen source nitrogen source nitrate 5mM -0.1 +0.1 -3.6 -0.1 -0.3
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 -0.0 -2.4 -0.8 +0.1
nitrogen source nitrogen source nitrite 1mM -0.1 +0.6 -3.1 -0.3 -0.1
nitrogen source nitrogen source nitrate 20mM +0.2 -0.3 -2.6 -0.1 +0.1
carbon source Decanoic (C) -0.1 -0.1 -2.6 +0.1 +0.0
carbon source 3-Methyl-2-Oxobutanoic Acid (C) -0.3 +0.1 -2.4 +0.1 -0.2
nitrogen source nitrogen source nitrate 20mM +0.1 -0.7 -2.4 +0.5 -0.1
carbon source Butyl stearate (C) +0.0 +0.4 -2.9 +0.1 -0.2
carbon source Octanoic (C) -0.1 +0.3 -2.6 +0.2 -0.0
nitrogen source nitrogen source nitrite 0.5mM +0.2 -0.6 -2.0 +0.5 -0.2
phage P. putida Sci small MOI 0.1 -0.1 -1.9 +0.5 -0.2 -0.3
carbon source Tween 20 (C) +0.2 +0.2 -2.6 +0.3 -0.0
carbon source Hexanoic (C) -0.0 +0.6 -2.3 +0.1 -0.0
nitrogen source 2,6-diaminopimelic (N) +0.3 -1.2 +1.9 -0.1 -0.1
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.0 -0.6 +2.0 -0.2 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.2 -0.8 +2.0 +0.3 -0.1
carbon source carbon source 4-vinylphenol 0.01 mM +0.2 -0.5 +1.8 +0.1 -0.5
carbon source 3-Hydroxydecanoic (C) -0.0 -1.2 +2.6 +0.0 +0.1
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.1 -0.6 +2.4 -0.2 -0.0
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.2 +0.7 +1.5 -0.5 -0.2
pH Valerolactone (C) pH 6.5 -0.1 -0.6 +2.5 +0.1 -0.1
pH pH 6 +0.4 +0.3 +2.0 -0.2 -0.1
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +0.2 -0.6 +2.8 -0.1 +0.1
micoeukaryotes C.elegans, mixed culture-2 +1.6 +0.2 +0.1 +0.5 +0.4
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.0 +0.3 +2.9 +0.3 +0.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 +1.7 +0.9 +0.3 +0.5 +0.5
nitrogen source 2,6-diaminopimelic (N) +0.2 +1.0 +2.1 +0.5 +0.1
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