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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3246 and PP_3247 are separated by 43 nucleotides
PP_3247 and PP_3248 are separated by 184 nucleotides
PP_3248 and PP_3249 are separated by 89 nucleotides
PP_3249 and PP_3250 are separated by 4 nucleotides
PP_3246: PP_3246 - Beta-carotene hydroxylase, at 3,680,247 to 3,680,714
_3246
PP_3247: PP_3247 - Transporter, bile acid/Na+ symporter family, at 3,680,758 to 3,681,651
_3247
PP_3248: PP_3248 - Dyp-type peroxidase family protein, at 3,681,836 to 3,682,699
_3248
PP_3249: PP_3249 - putative oxidoreductase, at 3,682,789 to 3,683,700
_3249
PP_3250: PP_3250 - putative Transporter, at 3,683,705 to 3,684,988
_3250
Group
Condition
PP
_3246
PP
_3247
PP
_3248
PP
_3249
PP
_3250
phage
P. putida Missley MOI 0.1
-0.1
-3.3
-0.6
-0.9
-0.1
carbon source
3-methyl-1-butanol (C)
-0.1
-0.1
-3.9
-0.3
-0.1
carbon source
3-methyl-1-butanol (C)
+0.3
-0.1
-4.1
-0.2
-0.1
nitrogen source
nitrogen source nitrate 5mM
-0.1
+0.1
-3.6
-0.1
-0.3
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.2
-0.0
-2.4
-0.8
+0.1
nitrogen source
nitrogen source nitrite 1mM
-0.1
+0.6
-3.1
-0.3
-0.1
nitrogen source
nitrogen source nitrate 20mM
+0.2
-0.3
-2.6
-0.1
+0.1
carbon source
Decanoic (C)
-0.1
-0.1
-2.6
+0.1
+0.0
carbon source
3-Methyl-2-Oxobutanoic Acid (C)
-0.3
+0.1
-2.4
+0.1
-0.2
nitrogen source
nitrogen source nitrate 20mM
+0.1
-0.7
-2.4
+0.5
-0.1
carbon source
Butyl stearate (C)
+0.0
+0.4
-2.9
+0.1
-0.2
carbon source
Octanoic (C)
-0.1
+0.3
-2.6
+0.2
-0.0
nitrogen source
nitrogen source nitrite 0.5mM
+0.2
-0.6
-2.0
+0.5
-0.2
phage
P. putida Sci small MOI 0.1
-0.1
-1.9
+0.5
-0.2
-0.3
carbon source
Tween 20 (C)
+0.2
+0.2
-2.6
+0.3
-0.0
carbon source
Hexanoic (C)
-0.0
+0.6
-2.3
+0.1
-0.0
nitrogen source
2,6-diaminopimelic (N)
+0.3
-1.2
+1.9
-0.1
-0.1
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.0
-0.6
+2.0
-0.2
-0.2
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-0.2
-0.8
+2.0
+0.3
-0.1
carbon source
carbon source 4-vinylphenol 0.01 mM
+0.2
-0.5
+1.8
+0.1
-0.5
carbon source
3-Hydroxydecanoic (C)
-0.0
-1.2
+2.6
+0.0
+0.1
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.1
-0.6
+2.4
-0.2
-0.0
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.2
+0.7
+1.5
-0.5
-0.2
pH
Valerolactone (C) pH 6.5
-0.1
-0.6
+2.5
+0.1
-0.1
pH
pH 6
+0.4
+0.3
+2.0
-0.2
-0.1
reactor
48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
+0.2
-0.6
+2.8
-0.1
+0.1
micoeukaryotes
C.elegans, mixed culture-2
+1.6
+0.2
+0.1
+0.5
+0.4
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
-0.0
+0.3
+2.9
+0.3
+0.1
micoeukaryotes
Acanthamoeba sp., muxed culture-2
+1.7
+0.9
+0.3
+0.5
+0.5
nitrogen source
2,6-diaminopimelic (N)
+0.2
+1.0
+2.1
+0.5
+0.1
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