Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3238 and PP_3239 are separated by 231 nucleotidesPP_3239 and PP_3240 are separated by 55 nucleotidesPP_3240 and PP_5592 are separated by 91 nucleotidesPP_5592 and PP_3241 are separated by 8 nucleotides PP_3238: PP_3238 - putative Transcriptional regulator PyrR, at 3,673,983 to 3,674,555 _3238 PP_3239: PP_3239 - putative cointegrate resolution protein T Tn4652, at 3,674,787 to 3,675,773 _3239 PP_3240: PP_3240 - conserved protein of unknown function, at 3,675,829 to 3,675,993 _3240 PP_5592: PP_5592 - conserved protein of unknown function, at 3,676,085 to 3,676,336 _5592 PP_3241: PP_3241 - conserved exported protein of unknown function, at 3,676,345 to 3,676,845 _3241
Group Condition PP_3238 PP_3239 PP_3240 PP_5592 PP_3241
phage PUT8 MOI 0.1 -0.0 -1.8 N.D. -0.1 -5.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.8 -6.5 N.D. -0.3 +0.9
nitrogen source D-2-Aminobutyric (N) -0.2 -2.1 N.D. -0.4 -3.4
temperature shift Temperature shift 30_to_10; with MOPS -0.1 +0.0 N.D. +0.0 -5.7
carbon source ferulate (C); with MOPS +0.3 -0.1 N.D. -0.4 -5.4
pH Growth at pH6 and (C) Trisodium citrate -0.4 +0.5 N.D. -0.3 -5.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.2 +0.0 N.D. -0.3 -5.0
carbon source D-Glucose (C); with MOPS +0.2 +0.2 N.D. +0.1 -5.8
phage PUT 8 MOI 1 -0.3 +1.0 N.D. -0.1 -5.7
nitrogen source 2,6-diaminopimelic (N) -0.4 -0.5 N.D. -0.0 -3.9
pH Growth at pH7 and (C) Trisodium citrate -0.3 -1.2 N.D. +0.1 -3.2
nitrogen source Urea (N); with MOPS +0.1 -1.5 N.D. -0.3 -2.6
pH Growth at pH9 and (C) Trisodium citrate +0.8 -4.0 N.D. -0.6 -0.4
nitrogen source no extra (N); with MOPS +0.2 -2.0 N.D. +0.2 -2.6
pH D-Glucose (C) pH 6.5 +0.1 -0.4 N.D. -0.2 -3.4
carbon source D-Glucose (C) -0.0 -0.3 N.D. -0.1 -3.3
phage P. putida Sci small MOI 0.1 +0.7 +0.1 N.D. -0.4 -4.1
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.8 -0.2 N.D. -0.0 -2.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -1.0 N.D. -0.1 -2.3
pH Growth at pH6 and (C) D-Glucose -0.2 -0.5 N.D. -0.3 -2.5
nitrogen source nitrate (N) -0.3 -0.3 N.D. -0.5 -2.4
temperature shift Temperature shift 30_to_21; with MOPS +0.0 -2.0 N.D. -0.2 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 -0.1 N.D. +0.2 -3.5
stress R2A with Fusaric 240 ug/mL -0.3 -0.7 N.D. +0.3 -2.2
carbon source DL-3-Hydroxybutyric (C) +0.3 -0.8 N.D. -0.3 -2.1
pH pH 5 +0.5 -0.5 N.D. +0.0 -2.7
carbon source ferulate (C); with MOPS +0.5 -0.6 N.D. +0.3 -2.7
nitrogen source nitrogen source nitrite 0.5mM +0.4 +1.0 N.D. -0.2 -3.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.5 +0.5 N.D. -0.2 +1.6
phage P. putida Sci Big MOI 0.1 -0.5 +1.8 N.D. -0.4 +0.6
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