Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3226 and PP_3227 are separated by 185 nucleotidesPP_3227 and PP_3228 are separated by 201 nucleotidesPP_3228 and PP_3229 are separated by 16 nucleotidesPP_3229 and PP_3230 are separated by 315 nucleotides PP_3226: PP_3226 - Acyl-CoA dehydrogenase-related protein, at 3,661,691 to 3,662,833 _3226 PP_3227: PP_3227 - Transcriptional regulator, LysR family, at 3,663,019 to 3,663,909 _3227 PP_3228: PP_3228 - putative Periplasmic aliphatic sulfonate-binding protein, at 3,664,111 to 3,665,076 _3228 PP_3229: PP_3229 - putative Periplasmic aliphatic sulfonate-binding protein, at 3,665,093 to 3,666,049 _3229 PP_3230: PP_3230 - Phosphoribosyl transferase domain protein, at 3,666,365 to 3,667,057 _3230
Group Condition PP_3226 PP_3227 PP_3228 PP_3229 PP_3230
nitrogen source NAG (N); with MOPS -0.3 -0.6 -0.5 -0.4 -0.8
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.1 -0.9 -0.6 -0.1 -0.6
nitrogen source nitrogen source nitrite 1mM -0.7 -0.6 -0.4 -0.3 -0.2
phage P. putida Sci small MOI 0.1 -0.2 -0.4 -1.1 -0.1 -0.1
carbon source L-tyrosine 5 mM (C) -0.2 -0.3 -0.9 -0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -1.1 -0.3 -0.0 -0.6
nitrogen source 2,6-diaminopimelic (N) -0.8 +0.7 -0.5 -0.1 -1.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.4 -1.3 -0.9 +0.2 -0.0
phage P. putida Sci small MOI 0.1 +0.1 -0.8 -1.0 +0.2 +0.0
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.1 -1.5 -0.3 +0.2 +0.3
nitrogen source nitrite (N) +0.0 -1.4 +0.0 -0.3 +0.2
nitrogen source Urea (N) +0.1 -1.2 -0.6 -0.3 +0.6
phage PUT8 MOI 0.1 -0.7 -0.3 +0.4 -0.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -1.1 -0.0 -0.2 +0.5
stress Growth with Chloride 500 mM; with MOPS -0.4 +0.4 -0.5 -0.4 -0.2
nitrogen source Ammonium chloride (N) +0.1 +0.4 -0.9 -0.1 -0.6
temperature shift Temperature shift 30_to_21; with MOPS +0.2 +0.3 -1.3 -0.3 +0.1
carbon source butyrate (C) -0.9 -0.6 +0.2 -0.0 +0.5
pH Valerolactone (C) pH 6.5 -1.3 +0.0 +0.4 +0.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 -0.3 +0.2 +0.5 -1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.0 -1.6 +0.5 +0.0 +0.4
temperature shift Temperature shift 30_to_21; with MOPS +0.1 -1.8 +0.7 +0.1 +0.6
pH Growth at pH7 and (C) D-Glucose -0.9 +0.7 -0.2 +0.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.3 +1.2 -0.0 -0.5
carbon source ferulate (C); with MOPS -0.7 +0.9 +0.0 -0.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +1.1 +0.5 -0.5 -0.9
pH pH 6 +0.3 -0.9 +0.6 -0.1 +0.6
nitrogen source D-2-Aminobutyric (N) +0.5 -0.5 +0.6 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.7 -0.1 -0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 +0.5 +0.8 +0.2 +0.2
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