Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3219 and PP_3220 are separated by 140 nucleotidesPP_3220 and PP_3221 overlap by 4 nucleotidesPP_3221 and PP_3222 are separated by 1 nucleotidesPP_3222 and PP_3223 overlap by 4 nucleotides PP_3219: PP_3219 - putative Alkansulfonate monooxygenase, at 3,653,532 to 3,654,632 _3219 PP_3220: PP_3220 - ABC transporter, ATP-binding protein, at 3,654,773 to 3,656,392 _3220 PP_3221: PP_3221 - ABC transporter, permease protein, at 3,656,389 to 3,657,297 _3221 PP_3222: PP_3222 - ABC transporter, permease protein, at 3,657,299 to 3,658,249 _3222 PP_3223: PP_3223 - ABC transporter, periplasmic binding protein, at 3,658,246 to 3,659,865 _3223
Group Condition PP_3219 PP_3220 PP_3221 PP_3222 PP_3223
carbon source Valerolactone (C) -0.5 -0.3 -0.3 -0.3 -0.2
phage PUT8 MOI 0.1 -0.1 -0.5 -0.4 -0.2 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.0 +0.2 -0.1 -0.6 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.1 -0.9 -0.6 +0.1
temperature Growth at 30C; with MOPS -0.1 +0.2 -1.0 +0.2 -0.3
phage P. putida South MOI 10 -0.4 +0.3 -0.5 -0.3 +0.1
carbon source Nonanoic (C) -0.1 -0.6 -0.2 -0.2 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.5 +0.2 -1.1 +0.4 +0.2
phage P. putida Sci small MOI 0.1 +0.0 +0.2 -0.4 +0.1 -0.6
nitrogen source 2,6-diaminopimelic (N) -0.0 +0.5 -1.1 +0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.1 -0.8 +0.7 +0.0
pH Growth at pH7 and (C) D-Glucose -0.2 -0.6 +0.7 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.3 -0.4 +0.4 +0.3 -0.4
carbon source ferulate (C); with MOPS -0.5 +0.4 -0.5 -0.0 +0.1
nitrogen source NAG (N); with MOPS -0.5 +0.4 -0.5 +0.1 +0.0
pH Growth at pH7 and (C) Trisodium citrate -0.3 -0.1 -0.5 +0.5 +0.1
temperature Growth at 25C -0.2 -0.4 +0.5 +0.2 -0.3
r2a control R2A control +0.3 +0.1 -0.7 +0.3 -0.1
nitrogen source D-2-Aminobutyric (N) -0.3 +0.3 -0.4 +0.2 +0.1
phage P. putida Sci small MOI 0.1 -0.4 +0.5 +0.5 -0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -0.5 +0.4 +0.3 +0.3
no stress control Growth with no stress -0.2 -0.4 +0.5 +0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.2 -0.7 +0.8 +0.3 +0.1
nitrogen source nitrogen source nitrite 1mM -0.1 +0.6 -0.6 +0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 +0.5 +0.2 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 -0.3 -0.1 +0.6 +0.5
r2a control R2A control +0.6 -0.1 -0.2 +0.5 +0.0
phage P. putida Missley MOI 0.1 +0.1 +0.4 -0.4 +0.5 +0.3
carbon source 1,3-Butandiol (C) +0.1 +0.4 +0.5 -0.2 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +0.3 +0.9 +0.1 +0.2
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