Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3204 and PP_3205 overlap by 4 nucleotidesPP_3205 and PP_3206 overlap by 10 nucleotidesPP_3206 and PP_3207 overlap by 4 nucleotidesPP_3207 and PP_3208 are separated by 79 nucleotides PP_3204: PP_3204 - conserved protein of unknown function, at 3,637,421 to 3,638,008 _3204 PP_3205: PP_3205 - Fumarylacetoacetate hydrolase family protein, at 3,638,005 to 3,638,997 _3205 PP_3206: PP_3206 - conserved protein of unknown function, at 3,638,988 to 3,639,974 _3206 PP_3207: PP_3207 - putative Cyclase, at 3,639,971 to 3,640,753 _3207 PP_3208: PP_3208 - Oxidoreductase, Pdr/VanB family, at 3,640,833 to 3,641,777 _3208
Group Condition PP_3204 PP_3205 PP_3206 PP_3207 PP_3208
pH Growth at pH6 and (C) D-Glucose -0.7 -0.5 -1.4 -0.1 -0.4
pH Growth at pH9 and (C) D-Glucose -1.4 -0.1 -0.8 +0.2 -0.3
carbon source Trisodium citrate (C) -1.2 -0.9 +0.1 +0.0 -0.3
carbon source D-Glucose (C) -0.5 -1.1 -0.2 +0.0 -0.3
carbon source ferulate (C); with MOPS -1.5 -0.5 -0.2 +0.2 -0.4
pH Growth at pH6 and (C) D-Glucose -1.2 -0.4 -0.1 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.3 -1.3 -0.4 +0.1 -0.1
nitrogen source NAG (N); with MOPS -1.4 +0.3 -0.7 +0.1 -0.1
pH Growth at pH7 and (C) Trisodium citrate -1.1 -0.7 -0.2 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -1.0 +0.2 +0.3 -0.4
carbon source D-Glucose (C); with MOPS +0.2 -0.8 -0.8 +0.3 -0.5
pH Growth at pH9 and (C) Trisodium citrate -1.4 +0.5 -0.4 +0.3 -0.3
phage P. putida Sci Big MOI 0.1 -0.7 +0.7 -0.3 -0.7 -0.2
phage P. putida Sci small MOI 0.1 +0.6 -0.2 -1.4 +0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.9 -0.0 +0.6 +0.1 +0.1
carbon source Myristic (C) -1.3 +0.4 -0.1 +0.2 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay -1.2 +0.6 +0.2 -0.2 -0.1
pH pH 7 +0.5 -0.2 -0.7 -0.7 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.8 -0.5 -0.1 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +1.1 -0.9 -0.4 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 -0.8 -0.1 -0.0 -0.1
pH pH 8 +0.6 -0.6 -0.4 +0.2 +0.5
pH pH 9 +1.2 +0.1 -0.8 -0.4 +0.3
carbon source carbon source Glycerol 108.6 mM +0.9 +0.3 -0.8 -0.3 +0.4
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.8 +0.2 -1.0 +0.3 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.8 +0.6 -0.3 -0.4 +0.2
carbon source carbon source 2-Deoxy-D-glucose 10 mM +1.0 +0.2 -0.3 -0.3 +0.5
phage P. putida PUT 7 MOI 1 +1.5 -0.1 +0.5 +0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.0 +1.1 -0.1 +0.2 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +1.5 -0.1 +0.1 +0.2
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