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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3204 and PP_3205 overlap by 4 nucleotides
PP_3205 and PP_3206 overlap by 10 nucleotides
PP_3206 and PP_3207 overlap by 4 nucleotides
PP_3207 and PP_3208 are separated by 79 nucleotides
PP_3204: PP_3204 - conserved protein of unknown function, at 3,637,421 to 3,638,008
_3204
PP_3205: PP_3205 - Fumarylacetoacetate hydrolase family protein, at 3,638,005 to 3,638,997
_3205
PP_3206: PP_3206 - conserved protein of unknown function, at 3,638,988 to 3,639,974
_3206
PP_3207: PP_3207 - putative Cyclase, at 3,639,971 to 3,640,753
_3207
PP_3208: PP_3208 - Oxidoreductase, Pdr/VanB family, at 3,640,833 to 3,641,777
_3208
Group
Condition
PP
_3204
PP
_3205
PP
_3206
PP
_3207
PP
_3208
pH
Growth at pH6 and (C) D-Glucose
-0.7
-0.5
-1.4
-0.1
-0.4
pH
Growth at pH9 and (C) D-Glucose
-1.4
-0.1
-0.8
+0.2
-0.3
carbon source
Trisodium citrate (C)
-1.2
-0.9
+0.1
+0.0
-0.3
carbon source
D-Glucose (C)
-0.5
-1.1
-0.2
+0.0
-0.3
carbon source
ferulate (C); with MOPS
-1.5
-0.5
-0.2
+0.2
-0.4
pH
Growth at pH6 and (C) D-Glucose
-1.2
-0.4
-0.1
-0.3
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.3
-1.3
-0.4
+0.1
-0.1
nitrogen source
NAG (N); with MOPS
-1.4
+0.3
-0.7
+0.1
-0.1
pH
Growth at pH7 and (C) Trisodium citrate
-1.1
-0.7
-0.2
+0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
-1.0
+0.2
+0.3
-0.4
carbon source
D-Glucose (C); with MOPS
+0.2
-0.8
-0.8
+0.3
-0.5
pH
Growth at pH9 and (C) Trisodium citrate
-1.4
+0.5
-0.4
+0.3
-0.3
phage
P. putida Sci Big MOI 0.1
-0.7
+0.7
-0.3
-0.7
-0.2
phage
P. putida Sci small MOI 0.1
+0.6
-0.2
-1.4
+0.1
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.9
-0.0
+0.6
+0.1
+0.1
carbon source
Myristic (C)
-1.3
+0.4
-0.1
+0.2
-0.2
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
-1.2
+0.6
+0.2
-0.2
-0.1
pH
pH 7
+0.5
-0.2
-0.7
-0.7
+0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.8
-0.5
-0.1
+1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+1.1
-0.9
-0.4
-0.3
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.3
-0.8
-0.1
-0.0
-0.1
pH
pH 8
+0.6
-0.6
-0.4
+0.2
+0.5
pH
pH 9
+1.2
+0.1
-0.8
-0.4
+0.3
carbon source
carbon source Glycerol 108.6 mM
+0.9
+0.3
-0.8
-0.3
+0.4
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.8
+0.2
-1.0
+0.3
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
+0.8
+0.6
-0.3
-0.4
+0.2
carbon source
carbon source 2-Deoxy-D-glucose 10 mM
+1.0
+0.2
-0.3
-0.3
+0.5
phage
P. putida PUT 7 MOI 1
+1.5
-0.1
+0.5
+0.2
-0.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+1.0
+1.1
-0.1
+0.2
+0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.6
+1.5
-0.1
+0.1
+0.2
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