Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3201 and PP_3202 are separated by 12 nucleotidesPP_3202 and PP_3203 overlap by 11 nucleotidesPP_3203 and PP_3204 are separated by 157 nucleotidesPP_3204 and PP_3205 overlap by 4 nucleotides PP_3201: PP_3201 - BNR domain protein, at 3,632,529 to 3,633,590 _3201 PP_3202: PP_3202 - putative Transporter, at 3,633,603 to 3,636,083 _3202 PP_3203: PP_3203 - Major facilitator family transporter, at 3,636,073 to 3,637,263 _3203 PP_3204: PP_3204 - conserved protein of unknown function, at 3,637,421 to 3,638,008 _3204 PP_3205: PP_3205 - Fumarylacetoacetate hydrolase family protein, at 3,638,005 to 3,638,997 _3205
Group Condition PP_3201 PP_3202 PP_3203 PP_3204 PP_3205
carbon source ferulate (C); with MOPS -0.2 -0.3 -0.3 -1.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -0.1 -0.4 -1.9 -0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM -0.9 -0.1 -0.0 -1.0 -0.3
carbon source Trisodium citrate (C) +0.4 -0.3 +0.2 -1.2 -0.9
pH Growth at pH6 and (C) Trisodium citrate +0.6 -0.4 -0.5 -0.8 -0.4
temperature Growth at 30C; with MOPS +0.6 -0.0 -0.9 -0.7 -0.4
pH Growth at pH7 and (C) D-Glucose -1.0 +0.2 +0.5 -0.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.3 +0.3 -0.8 -1.0
pH Growth at pH6 and (C) D-Glucose +0.9 -0.1 -0.3 -1.2 -0.4
pH Growth at pH7 and (C) Trisodium citrate +0.8 -0.1 +0.1 -1.1 -0.7
nitrogen source NAG (N); with MOPS +0.8 -0.2 -0.2 -1.4 +0.3
carbon source Tween 20 (C) +0.8 -0.4 +0.0 -0.9 -0.3
phage P. putida Sci small MOI 0.1 -1.9 +0.3 -0.2 +0.7 +0.4
nitrogen source nitrogen source nitrite 0.5mM -0.2 -0.6 +0.1 +0.8 -0.6
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.6 -0.1 -0.0 -1.2 +0.6
pH Growth at pH7 and (C) D-Glucose +0.9 -0.6 -0.1 -0.5 +0.2
pH Growth at pH6 and (C) D-Glucose +1.1 -0.3 +0.3 -0.7 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.1 -0.8 +0.5 +1.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.3 +0.3 -0.4 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 -0.2 +0.5 +0.1 -0.8
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.0 -0.2 -0.7 +0.7 +0.7
nitrogen source Urea (N) +0.6 -0.2 -0.3 +0.6 +0.6
carbon source carbon source Glycerol 108.6 mM -0.7 +0.4 +0.4 +0.9 +0.3
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.7 -0.0 +0.2 -0.3 +1.0
pH pH 9 -0.4 +0.2 +0.6 +1.2 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.0 +0.3 +0.6 +1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 -0.1 +0.1 +1.0 +1.1
phage P. putida PUT 7 MOI 1 +0.6 +0.3 +0.4 +1.5 -0.1
carbon source Delta-Undecalactone (C) +1.3 +1.7 +1.3 +0.0 -0.5
carbon source Delta-Undecalactone (C) +1.6 +1.7 +1.7 -0.6 -0.2
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