Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3192 and PP_3193 are separated by 225 nucleotidesPP_3193 and PP_3194 overlap by 13 nucleotidesPP_3194 and PP_3195 are separated by 172 nucleotidesPP_3195 and PP_3196 are separated by 76 nucleotides PP_3192: PP_3192 - conserved protein of unknown function, at 3,621,432 to 3,622,037 _3192 PP_3193: PP_3193 - conserved protein of unknown function, at 3,622,263 to 3,622,721 _3193 PP_3194: PP_3194 - conserved protein of unknown function, at 3,622,709 to 3,624,052 _3194 PP_3195: PP_3195 - conserved protein of unknown function, at 3,624,225 to 3,625,487 _3195 PP_3196: PP_3196 - Nodulation protein d1, at 3,625,564 to 3,626,502 _3196
Group Condition PP_3192 PP_3193 PP_3194 PP_3195 PP_3196
phage P. putida Missley MOI 0.1 -2.0 N.D. -0.1 -0.1 -0.0
temperature Growth at 25C -0.3 N.D. -0.4 -0.7 -0.3
stress R2A with Fusaric 120 ug/mL -0.8 N.D. -0.4 +0.1 -0.4
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.6 N.D. -0.6 -0.5 +0.1
carbon source Valerolactone (C) -1.7 N.D. -0.1 +0.1 +0.1
nitrogen source Urea (N) -0.7 N.D. -0.6 -0.4 +0.3
stress R2A with Fusaric 480 ug/mL -1.1 N.D. +0.1 -0.4 +0.0
carbon source D-Glucaric (C) -1.0 N.D. -0.3 -0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.4 N.D. +0.0 -0.2 +0.4
nitrogen source 2,6-diaminopimelic (N) -1.7 N.D. -0.1 +0.2 +0.5
pH Growth at pH7 and (C) D-Glucose -1.0 N.D. +0.3 -0.6 +0.2
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.7 N.D. +0.3 -0.0 -0.5
nitrogen source nitrite (N) -0.7 N.D. +0.3 -0.3 -0.3
carbon source D-Glucuronic gamma lactone (C) -0.5 N.D. -0.7 +0.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.1 N.D. +0.0 +0.2 +0.3
temperature Growth at 25C; with MOPS -0.7 N.D. +0.5 -0.2 -0.2
nitrogen source 2,6-diaminopimelic (N) -1.1 N.D. +0.4 +0.3 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.6 N.D. -0.8 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -1.0 N.D. +0.5 +0.1 +0.0
carbon source Myristic (C) -0.0 N.D. -1.2 +0.5 +0.4
temperature shift Temperature shift 30_to_34; with MOPS -0.4 N.D. +0.4 -0.6 +0.3
phage P. put South MOI 10 +0.1 N.D. -0.8 -0.2 +0.5
temperature Growth at 25C -1.0 N.D. +0.5 +0.0 +0.4
pH pH 7 +1.1 N.D. -0.3 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 N.D. -0.7 -0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 N.D. +0.4 +0.6 -0.2
nitrogen source D-2-Aminobutyric (N) +1.2 N.D. -0.8 -0.0 +0.0
phage P. putida Sci Big MOI 0.1 +1.5 N.D. -0.4 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. -0.3 +0.8 -0.1
pH pH 8 +1.3 N.D. +0.7 -0.1 +0.2
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