Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3144 and PP_3145 are separated by 87 nucleotidesPP_3145 and PP_3146 overlap by 4 nucleotidesPP_3146 and PP_3147 are separated by 13 nucleotidesPP_3147 and PP_3148 are separated by 80 nucleotides PP_3144: PP_3144 - L-serine dehydratase, at 3,560,246 to 3,561,622 _3144 PP_3145: PP_3145 - conserved protein of unknown function, at 3,561,710 to 3,562,531 _3145 PP_3146: PP_3146 - putative Oxidoreductase, at 3,562,528 to 3,563,823 _3146 PP_3147: PP_3147 - putrescine-binding periplasmic protein, at 3,563,837 to 3,564,925 _3147 PP_3148: PP_3148 - putative Glutamine synthetase, at 3,565,006 to 3,566,364 _3148
Group Condition PP_3144 PP_3145 PP_3146 PP_3147 PP_3148
nitrogen source spermidine (N) +0.0 +0.0 -4.6 -2.6 -3.2
nitrogen source spermidine (N) +0.1 +0.1 -4.6 -2.6 -3.1
phage P. putida Sci small MOI 0.1 -0.2 -2.2 +0.4 -0.5 -0.6
phage P. putida Sci Big MOI 0.1 -1.3 -0.2 -0.1 -0.9 -0.3
nitrogen source L-Serine (N) -2.3 -0.0 -0.2 +0.0 -0.0
nitrogen source L-Serine (N) -2.3 -0.0 -0.0 -0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.4 -0.1 -0.3 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 -0.3 -0.5 -0.3 +0.1
no stress control Growth with no stress -0.9 -0.5 +0.1 -0.2 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.6 -0.4 +0.3 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.2 -0.1 +0.3 -0.5
temperature shift Temperature shift 10_to_25; with MOPS -0.7 -0.3 +0.1 -0.3 +0.4
carbon source L-Phenylalanine 5 mM (C) -0.3 -0.8 +0.2 -0.1 +0.3
carbon source Valerolactone (C) -0.3 -0.6 -0.6 +0.5 +0.3
temperature shift Temperature shift 30_to_25 +0.5 -0.7 +0.2 +0.0 -0.2
carbon source Valeric (C) +0.3 +0.3 +0.1 -0.7 +0.2
phage P. putida PUT10 MOI 0.1 +0.8 -0.5 +0.1 +0.3 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.4 +0.4 -0.1 +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +1.0 +0.2 +0.1 -0.3
phage P. putida PUT10 MOI 10 +1.0 +0.3 -0.1 +0.1 -0.4
carbon source carbon source 4-vinylphenol 0.1 mM +0.5 -0.1 +0.5 -0.2 +0.3
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.9 +0.2 +0.1 -0.3 +0.2
pH pH 9 +0.5 +0.4 +0.7 -0.3 +0.2
pH pH 7 +0.6 +0.5 -0.2 +0.2 +0.3
pH Growth at pH6 and (C) D-Glucose +0.1 +0.5 +0.4 +0.5 -0.1
nitrogen source nitrate (N) +0.5 +0.4 +0.5 -0.0 +0.2
pH pH 6 +0.6 +0.4 +0.5 -0.1 +0.1
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.4 +0.7 +0.1 -0.0 +0.4
nitrogen source Urea (N); with MOPS +0.8 +0.3 +0.1 +0.2 +0.3
phage PUT 8 MOI 1 +0.8 +0.4 +0.2 +0.2 +0.0
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