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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3144 and PP_3145 are separated by 87 nucleotides
PP_3145 and PP_3146 overlap by 4 nucleotides
PP_3146 and PP_3147 are separated by 13 nucleotides
PP_3147 and PP_3148 are separated by 80 nucleotides
PP_3144: PP_3144 - L-serine dehydratase, at 3,560,246 to 3,561,622
_3144
PP_3145: PP_3145 - conserved protein of unknown function, at 3,561,710 to 3,562,531
_3145
PP_3146: PP_3146 - putative Oxidoreductase, at 3,562,528 to 3,563,823
_3146
PP_3147: PP_3147 - putrescine-binding periplasmic protein, at 3,563,837 to 3,564,925
_3147
PP_3148: PP_3148 - putative Glutamine synthetase, at 3,565,006 to 3,566,364
_3148
Group
Condition
PP
_3144
PP
_3145
PP
_3146
PP
_3147
PP
_3148
nitrogen source
spermidine (N)
+0.0
+0.0
-4.6
-2.6
-3.2
nitrogen source
spermidine (N)
+0.1
+0.1
-4.6
-2.6
-3.1
phage
P. putida Sci small MOI 0.1
-0.2
-2.2
+0.4
-0.5
-0.6
phage
P. putida Sci Big MOI 0.1
-1.3
-0.2
-0.1
-0.9
-0.3
nitrogen source
L-Serine (N)
-2.3
-0.0
-0.2
+0.0
-0.0
nitrogen source
L-Serine (N)
-2.3
-0.0
-0.0
-0.1
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.4
-0.1
-0.3
-0.3
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.5
-0.3
-0.5
-0.3
+0.1
no stress control
Growth with no stress
-0.9
-0.5
+0.1
-0.2
+0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.6
-0.6
-0.4
+0.3
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
-0.2
-0.1
+0.3
-0.5
temperature shift
Temperature shift 10_to_25; with MOPS
-0.7
-0.3
+0.1
-0.3
+0.4
carbon source
L-Phenylalanine 5 mM (C)
-0.3
-0.8
+0.2
-0.1
+0.3
carbon source
Valerolactone (C)
-0.3
-0.6
-0.6
+0.5
+0.3
temperature shift
Temperature shift 30_to_25
+0.5
-0.7
+0.2
+0.0
-0.2
carbon source
Valeric (C)
+0.3
+0.3
+0.1
-0.7
+0.2
phage
P. putida PUT10 MOI 0.1
+0.8
-0.5
+0.1
+0.3
-0.2
micoeukaryotes
Tetrahymena sp., Mixed culture-2
+0.4
+0.4
-0.1
+0.3
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+1.0
+0.2
+0.1
-0.3
phage
P. putida PUT10 MOI 10
+1.0
+0.3
-0.1
+0.1
-0.4
carbon source
carbon source 4-vinylphenol 0.1 mM
+0.5
-0.1
+0.5
-0.2
+0.3
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
+0.9
+0.2
+0.1
-0.3
+0.2
pH
pH 9
+0.5
+0.4
+0.7
-0.3
+0.2
pH
pH 7
+0.6
+0.5
-0.2
+0.2
+0.3
pH
Growth at pH6 and (C) D-Glucose
+0.1
+0.5
+0.4
+0.5
-0.1
nitrogen source
nitrate (N)
+0.5
+0.4
+0.5
-0.0
+0.2
pH
pH 6
+0.6
+0.4
+0.5
-0.1
+0.1
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.4
+0.7
+0.1
-0.0
+0.4
nitrogen source
Urea (N); with MOPS
+0.8
+0.3
+0.1
+0.2
+0.3
phage
PUT 8 MOI 1
+0.8
+0.4
+0.2
+0.2
+0.0
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