Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3081 and PP_5560 are separated by 123 nucleotidesPP_5560 and PP_3082 are separated by 81 nucleotidesPP_3082 and PP_3083 are separated by 360 nucleotidesPP_3083 and PP_3084 are separated by 132 nucleotides PP_3081: PP_3081 - conserved protein of unknown function, at 3,466,349 to 3,466,825 _3081 PP_5560: PP_5560 - conserved exported protein of unknown function, at 3,466,949 to 3,467,257 _5560 PP_3082: PP_3082 - putative Membrane protein, at 3,467,339 to 3,468,703 _3082 PP_3083: PP_3083 - putative Membrane protein, at 3,469,064 to 3,469,939 _3083 PP_3084: PP_3084 - putative Outer membrane ferric siderophore receptor, at 3,470,072 to 3,472,531 _3084
Group Condition PP_3081 PP_5560 PP_3082 PP_3083 PP_3084
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.8 N.D. -0.4 -0.9 -0.1
phage P. putida PUT 7 MOI 1 -1.6 N.D. +0.3 -0.8 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.3 N.D. -0.5 +0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.4 N.D. -0.2 +0.1 -0.0
temperature Growth at 30C; with MOPS -0.2 N.D. -0.2 -1.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 N.D. -0.7 -0.5 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.9 N.D. -0.3 -0.2 -0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 N.D. -0.7 -0.0 +0.1
carbon source 4-Hydroxyvalerate (C) (40mM) -0.6 N.D. +0.1 -0.6 -0.0
pH D-Glucose (C) pH 6.5 -0.8 N.D. -0.4 +0.5 -0.1
nitrogen source NAG (N); with MOPS -1.2 N.D. -0.1 +0.7 +0.0
phage P. putida Sci small MOI 0.1 +0.2 N.D. +0.5 -0.8 -0.3
pH Growth at pH6 and (C) Trisodium citrate -0.8 N.D. -0.1 +0.4 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 N.D. +0.6 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 N.D. +0.6 +0.3 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.7 N.D. +0.1 +0.5 -0.2
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -0.9 N.D. +0.3 +0.4 -0.1
nitrogen source no extra (N); with MOPS -0.8 N.D. +0.2 +0.7 -0.1
nitrogen source nitrate (N) -0.8 N.D. +0.4 +0.2 +0.3
carbon source ferulate (C); with MOPS -0.9 N.D. -0.1 +0.8 +0.4
pH Growth at pH9 and (C) Trisodium citrate -0.6 N.D. +0.1 +0.7 +0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 N.D. +0.1 +0.8 -0.2
pH Valerolactone (C) pH 6.5 -0.7 N.D. +0.2 +0.6 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.8 N.D. +0.3 -0.6 +0.0
pH pH 9 +0.7 N.D. -0.1 +0.3 -0.2
temperature shift Temperature shift 30_to_25; with MOPS +0.9 N.D. -0.2 +0.5 -0.1
nitrogen source NAG (N); with MOPS +0.5 N.D. -0.1 +0.6 +0.4
pH Growth at pH7 and (C) D-Glucose +0.2 N.D. +0.2 +1.0 +0.1
phage P. putida Sci small MOI 0.1 +1.0 N.D. +0.3 +0.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.7 N.D. +0.3 +0.4 +0.1
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