Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3060 and PP_3061 are separated by 45 nucleotidesPP_3061 and PP_3062 are separated by 132 nucleotidesPP_3062 and PP_3063 are separated by 9 nucleotidesPP_3063 and PP_3064 overlap by 26 nucleotides PP_3060: PP_3060 - putative tail sheath protein, at 3,440,258 to 3,440,767 _3060 PP_3061: PP_3061 - conserved protein of unknown function, at 3,440,813 to 3,441,109 _3061 PP_3062: PP_3062 - putative Pyocin R2_PP, tail length determination protein, at 3,441,242 to 3,444,511 _3062 PP_3063: PP_3063 - Pyocin R2_PP, tail formation protein, at 3,444,521 to 3,445,366 _3063 PP_3064: PP_3064 - Phage tail protein, at 3,445,341 to 3,445,547 _3064
Group Condition PP_3060 PP_3061 PP_3062 PP_3063 PP_3064
temperature Growth at 30C; with MOPS -0.1 +0.3 +0.2 -0.3 -5.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 -0.8 -0.2 -0.3 -3.9
phage P. putida Missley MOI 0.1 -0.0 -0.9 -0.1 -0.9 -1.9
nitrogen source nitrogen source nitrite 0.5mM -0.7 +0.1 +0.5 -0.8 -2.1
pH pH 6 -0.3 +0.1 -0.1 -0.6 -2.1
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.7 +0.4 -0.1 -0.5 -2.0
phage P. putida Sci Big MOI 0.1 -1.0 -0.7 +0.3 -0.9 -0.5
pH Growth at pH7 and (C) D-Glucose -0.2 -0.4 +0.0 -0.1 -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 -0.0 +0.1 -0.1 -2.6
carbon source carbon source 4-vinylphenol 0.01 mM -0.4 -0.4 -0.0 -0.6 -1.3
nitrogen source nitrogen source nitrate 5mM -0.0 -0.1 +0.2 -0.1 -2.4
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM -0.1 -0.1 +0.2 -0.1 -2.4
carbon source carbon source D-Glucose 55.5 mM -1.3 +0.1 +0.0 -0.1 -1.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 -1.3 -0.2 +0.2 -0.6
carbon source 4-Aminobutyric 10 mM (C) +0.1 -0.7 +0.1 +0.0 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.0 -0.0 -0.5 -1.8
pH D-Glucose (C) pH 7.5 -0.0 +0.2 +0.1 -0.0 -2.2
carbon source carbon source 3-O-methyl-glucose 50 mM -0.6 +0.3 -0.1 -0.9 -0.6
no stress control Growth with no stress +0.6 -0.1 +0.2 +0.0 -2.6
nitrogen source nitrogen source nitrite 1mM -0.2 -0.1 +0.4 -0.9 -0.9
nitrogen source nitrogen source nitrite 0.5mM -0.3 -0.1 +0.4 -0.2 -1.4
phage PUT 8 MOI 1 -0.6 +0.5 -0.4 -0.1 -1.1
nitrogen source 2,6-diaminopimelic (N) -0.4 -0.8 -0.0 -1.4 +1.1
carbon source carbon source 3-O-methyl-glucose 10 mM -0.1 +0.8 -0.3 -0.4 -1.2
carbon source D-Glucose (C) -0.2 +0.4 +0.3 -0.7 -1.0
pH Growth at pH6 and (C) D-Glucose +0.5 +0.4 +0.0 -0.3 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 +0.4 +0.2 +0.1 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.8 -0.3 +0.3 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.7 -0.8 +0.1 +0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.7 -0.1 +0.1 +1.8
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