Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3043 and PP_3044 overlap by 4 nucleotidesPP_3044 and PP_3045 overlap by 4 nucleotidesPP_3045 and PP_3046 are separated by 2 nucleotidesPP_3046 and PP_3047 are separated by 61 nucleotides PP_3043: PP_3043 - conserved protein of unknown function, at 3,428,090 to 3,428,296 _3043 PP_3044: PP_3044 - Phage portal protein, lambda family, at 3,428,293 to 3,429,786 _3044 PP_3045: PP_3045 - putative ClpP protease, at 3,429,783 to 3,430,946 _3045 PP_3046: PP_3046 - conserved protein of unknown function, at 3,430,949 to 3,431,296 _3046 PP_3047: PP_3047 - conserved protein of unknown function, at 3,431,358 to 3,432,353 _3047
Group Condition PP_3043 PP_3044 PP_3045 PP_3046 PP_3047
phage P. putida PUT 7 MOI 1 -4.4 +0.1 -0.6 N.D. -0.3
nitrogen source Propandiamine (N) -3.0 -0.3 -0.4 N.D. +0.1
phage P. putida Sci small MOI 0.1 -1.1 -0.7 -0.4 N.D. -0.5
phage P. putida Missley MOI 0.1 -1.2 -0.1 +0.2 N.D. -1.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.1 +0.4 -0.7 N.D. +0.3
r2a control with 0.2x vogels R2A control with 0.2X Vogels -2.0 +0.3 -0.2 N.D. -0.1
nitrogen source Propiolactam (N) -1.6 -0.1 +0.2 N.D. -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.9 +0.1 +0.3 N.D. -0.1
nitrogen source NAG (N); with MOPS -2.3 +0.3 +0.2 N.D. +0.1
stress Growth with Chloride 500 mM -1.4 -0.1 -0.2 N.D. +0.2
phage P. putida Sci Big MOI 0.1 +0.4 -1.0 -0.5 N.D. -0.4
carbon source D-Lysine 10 mM (C) -1.8 +0.1 +0.1 N.D. +0.1
phage P. putida Sci small MOI 0.1 +0.4 -1.0 +0.3 N.D. -1.1
carbon source Acetate Carbon Source (20mM) -1.2 +0.2 -0.2 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 -0.3 +0.0 N.D. +0.4
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.5 +0.1 -0.1 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.2 +0.2 +0.2 N.D. -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.6 +0.3 +0.3 N.D. +0.3
nitrogen source 2,6-diaminopimelic (N) +0.7 -0.7 -0.3 N.D. -0.1
nitrogen source D-2-Aminobutyric (N) +1.4 +0.0 -0.7 N.D. +0.0
carbon source L-tyrosine 5 mM (C) +1.5 -0.1 -0.3 N.D. -0.1
pH Growth at pH9 and (C) Trisodium citrate +0.9 +0.4 +0.3 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.6 +0.2 -0.6 N.D. -0.1
carbon source Delta-Nonalactone (C) +1.6 -0.2 -0.0 N.D. -0.0
pH Growth at pH6 and (C) D-Glucose +1.6 -0.0 -0.3 N.D. +0.1
carbon source Valerolactone (C) +2.0 +0.1 -0.3 N.D. -0.4
carbon source D-Glucaric (C) +1.5 -0.1 +0.2 N.D. -0.0
carbon source ferulate (C); with MOPS +1.9 +0.2 -0.1 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.1 -0.2 +0.2 N.D. +0.1
nitrogen source nitrogen source nitrite 1mM +1.6 +0.2 +0.3 N.D. +0.3
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