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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3043 and PP_3044 overlap by 4 nucleotides
PP_3044 and PP_3045 overlap by 4 nucleotides
PP_3045 and PP_3046 are separated by 2 nucleotides
PP_3046 and PP_3047 are separated by 61 nucleotides
PP_3043: PP_3043 - conserved protein of unknown function, at 3,428,090 to 3,428,296
_3043
PP_3044: PP_3044 - Phage portal protein, lambda family, at 3,428,293 to 3,429,786
_3044
PP_3045: PP_3045 - putative ClpP protease, at 3,429,783 to 3,430,946
_3045
PP_3046: PP_3046 - conserved protein of unknown function, at 3,430,949 to 3,431,296
_3046
PP_3047: PP_3047 - conserved protein of unknown function, at 3,431,358 to 3,432,353
_3047
Group
Condition
PP
_3043
PP
_3044
PP
_3045
PP
_3046
PP
_3047
phage
P. putida PUT 7 MOI 1
-4.4
+0.1
-0.6
N.D.
-0.3
nitrogen source
Propandiamine (N)
-3.0
-0.3
-0.4
N.D.
+0.1
phage
P. putida Sci small MOI 0.1
-1.1
-0.7
-0.4
N.D.
-0.5
phage
P. putida Missley MOI 0.1
-1.2
-0.1
+0.2
N.D.
-1.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-2.1
+0.4
-0.7
N.D.
+0.3
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
-2.0
+0.3
-0.2
N.D.
-0.1
nitrogen source
Propiolactam (N)
-1.6
-0.1
+0.2
N.D.
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-1.9
+0.1
+0.3
N.D.
-0.1
nitrogen source
NAG (N); with MOPS
-2.3
+0.3
+0.2
N.D.
+0.1
stress
Growth with Chloride 500 mM
-1.4
-0.1
-0.2
N.D.
+0.2
phage
P. putida Sci Big MOI 0.1
+0.4
-1.0
-0.5
N.D.
-0.4
carbon source
D-Lysine 10 mM (C)
-1.8
+0.1
+0.1
N.D.
+0.1
phage
P. putida Sci small MOI 0.1
+0.4
-1.0
+0.3
N.D.
-1.1
carbon source
Acetate Carbon Source (20mM)
-1.2
+0.2
-0.2
N.D.
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.4
-0.3
+0.0
N.D.
+0.4
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-1.5
+0.1
-0.1
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-1.2
+0.2
+0.2
N.D.
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.6
+0.3
+0.3
N.D.
+0.3
nitrogen source
2,6-diaminopimelic (N)
+0.7
-0.7
-0.3
N.D.
-0.1
nitrogen source
D-2-Aminobutyric (N)
+1.4
+0.0
-0.7
N.D.
+0.0
carbon source
L-tyrosine 5 mM (C)
+1.5
-0.1
-0.3
N.D.
-0.1
pH
Growth at pH9 and (C) Trisodium citrate
+0.9
+0.4
+0.3
N.D.
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.6
+0.2
-0.6
N.D.
-0.1
carbon source
Delta-Nonalactone (C)
+1.6
-0.2
-0.0
N.D.
-0.0
pH
Growth at pH6 and (C) D-Glucose
+1.6
-0.0
-0.3
N.D.
+0.1
carbon source
Valerolactone (C)
+2.0
+0.1
-0.3
N.D.
-0.4
carbon source
D-Glucaric (C)
+1.5
-0.1
+0.2
N.D.
-0.0
carbon source
ferulate (C); with MOPS
+1.9
+0.2
-0.1
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.1
-0.2
+0.2
N.D.
+0.1
nitrogen source
nitrogen source nitrite 1mM
+1.6
+0.2
+0.3
N.D.
+0.3
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