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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3037 and PP_3038 overlap by 11 nucleotides
PP_3038 and PP_3039 overlap by 4 nucleotides
PP_3039 and PP_3040 are separated by 511 nucleotides
PP_3040 and PP_3041 are separated by 164 nucleotides
PP_3037: PP_3037 - C4-type zinc finger protein, DksA/TraR family, at 3,421,635 to 3,421,853
_3037
PP_3038: PP_3038 - Pyocin R2_PP, traC domain protein, at 3,421,843 to 3,424,053
_3038
PP_3039: PP_3039 - conserved protein of unknown function, at 3,424,050 to 3,424,412
_3039
PP_3040: PP_3040 - Pyocin R2_PP, holin, at 3,424,924 to 3,425,271
_3040
PP_3041: PP_3041 - conserved protein of unknown function, at 3,425,436 to 3,426,047
_3041
Group
Condition
PP
_3037
PP
_3038
PP
_3039
PP
_3040
PP
_3041
phage
P. putida Sci small MOI 0.1
-6.2
-0.2
-0.4
-1.2
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.6
-0.1
+0.2
-0.3
-1.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.4
-0.1
-0.3
-0.6
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-4.4
+0.1
+0.5
+0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-3.1
-0.1
+0.0
-0.5
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-2.8
+0.3
+0.1
-0.7
-0.4
nitrogen source
nitrate (N)
-1.7
-0.0
-0.2
-1.0
-0.3
carbon source
Valeric (C)
-3.6
+0.0
+0.0
+0.1
+0.2
phage
P. putida PUT 7 MOI 1
-2.0
-0.6
-0.2
-0.4
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.4
-0.1
-0.2
-0.5
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-2.4
-0.3
+0.2
+0.1
+0.2
no stress control
Growth with no stress
-0.9
+0.1
+0.0
-1.2
+0.0
pH
pH 7
-0.9
-0.0
+0.2
-0.8
-0.3
carbon source
carbon source D-Glucose 55.5 mM
+0.1
-0.7
-0.4
-0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.5
+0.4
+0.0
-0.5
-0.2
nitrogen source
nitrogen source nitrate 5mM
-1.3
-0.3
-0.2
+0.3
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.7
+0.3
-0.4
-0.8
+0.0
temperature shift
Temperature shift 30_to_25; with MOPS
-0.5
-0.5
-0.1
-0.7
+0.4
carbon source
Myristic (C)
-1.6
+0.0
+0.4
-0.0
+0.1
carbon source
carbon source 3-O-methyl-glucose 10 mM
+0.5
-0.1
-0.0
-0.8
-0.6
pH
pH 6
+0.6
-0.1
+0.2
-1.4
-0.1
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-1.3
-0.2
+0.3
+0.2
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-1.6
+0.2
+0.2
+0.6
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.5
+0.0
+0.2
-1.0
+0.4
phage
P. putida Sci Big MOI 0.1
-0.3
-0.7
-0.5
+1.2
+0.7
pH
Growth at pH6 and (C) D-Glucose
+1.0
+0.2
+0.1
-0.7
+0.1
carbon source
Glucose (C) (20mM)
+0.7
+0.1
+0.3
-0.7
+0.3
nitrogen source
2,6-diaminopimelic (N)
+1.1
+0.2
+0.2
+0.2
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
-0.1
+0.1
+1.1
+0.3
nitrogen source
D-2-Aminobutyric (N)
+0.9
+0.1
-0.2
+0.2
+1.0
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