Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3037 and PP_3038 overlap by 11 nucleotidesPP_3038 and PP_3039 overlap by 4 nucleotidesPP_3039 and PP_3040 are separated by 511 nucleotidesPP_3040 and PP_3041 are separated by 164 nucleotides PP_3037: PP_3037 - C4-type zinc finger protein, DksA/TraR family, at 3,421,635 to 3,421,853 _3037 PP_3038: PP_3038 - Pyocin R2_PP, traC domain protein, at 3,421,843 to 3,424,053 _3038 PP_3039: PP_3039 - conserved protein of unknown function, at 3,424,050 to 3,424,412 _3039 PP_3040: PP_3040 - Pyocin R2_PP, holin, at 3,424,924 to 3,425,271 _3040 PP_3041: PP_3041 - conserved protein of unknown function, at 3,425,436 to 3,426,047 _3041
Group Condition PP_3037 PP_3038 PP_3039 PP_3040 PP_3041
phage P. putida Sci small MOI 0.1 -6.2 -0.2 -0.4 -1.2 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.6 -0.1 +0.2 -0.3 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.4 -0.1 -0.3 -0.6 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -4.4 +0.1 +0.5 +0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -3.1 -0.1 +0.0 -0.5 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.8 +0.3 +0.1 -0.7 -0.4
nitrogen source nitrate (N) -1.7 -0.0 -0.2 -1.0 -0.3
carbon source Valeric (C) -3.6 +0.0 +0.0 +0.1 +0.2
phage P. putida PUT 7 MOI 1 -2.0 -0.6 -0.2 -0.4 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.4 -0.1 -0.2 -0.5 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -2.4 -0.3 +0.2 +0.1 +0.2
no stress control Growth with no stress -0.9 +0.1 +0.0 -1.2 +0.0
pH pH 7 -0.9 -0.0 +0.2 -0.8 -0.3
carbon source carbon source D-Glucose 55.5 mM +0.1 -0.7 -0.4 -0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 +0.4 +0.0 -0.5 -0.2
nitrogen source nitrogen source nitrate 5mM -1.3 -0.3 -0.2 +0.3 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.7 +0.3 -0.4 -0.8 +0.0
temperature shift Temperature shift 30_to_25; with MOPS -0.5 -0.5 -0.1 -0.7 +0.4
carbon source Myristic (C) -1.6 +0.0 +0.4 -0.0 +0.1
carbon source carbon source 3-O-methyl-glucose 10 mM +0.5 -0.1 -0.0 -0.8 -0.6
pH pH 6 +0.6 -0.1 +0.2 -1.4 -0.1
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.3 -0.2 +0.3 +0.2 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.6 +0.2 +0.2 +0.6 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 +0.0 +0.2 -1.0 +0.4
phage P. putida Sci Big MOI 0.1 -0.3 -0.7 -0.5 +1.2 +0.7
pH Growth at pH6 and (C) D-Glucose +1.0 +0.2 +0.1 -0.7 +0.1
carbon source Glucose (C) (20mM) +0.7 +0.1 +0.3 -0.7 +0.3
nitrogen source 2,6-diaminopimelic (N) +1.1 +0.2 +0.2 +0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.1 +0.1 +1.1 +0.3
nitrogen source D-2-Aminobutyric (N) +0.9 +0.1 -0.2 +0.2 +1.0
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