Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3033 and PP_3034 are separated by 78 nucleotidesPP_3034 and PP_3035 overlap by 90 nucleotidesPP_3035 and PP_5554 are separated by 108 nucleotidesPP_5554 and PP_3036 are separated by 71 nucleotides PP_3033: PP_3033 - putative transcriptional repressor, CI/C2 family, at 3,419,453 to 3,420,184 _3033 PP_3034: PP_3034 - putative Regulator protein, at 3,420,263 to 3,420,526 _3034 PP_3035: PP_3035 - conserved protein of unknown function, at 3,420,437 to 3,420,610 _3035 PP_5554: PP_5554 - protein of unknown function, at 3,420,719 to 3,420,901 _5554 PP_3036: PP_3036 - conserved protein of unknown function, at 3,420,973 to 3,421,152 _3036
Group Condition PP_3033 PP_3034 PP_3035 PP_5554 PP_3036
phage P. putida Sci small MOI 0.1 N.D. -0.6 -4.4 -0.6 -1.1
phage P. putida PUT 7 MOI 1 N.D. -1.5 -1.9 -1.3 -0.1
nitrogen source no extra (N); with MOPS N.D. -2.0 -1.2 -0.3 -0.6
carbon source D-Glucose (C); with MOPS N.D. -0.7 -1.5 -0.8 -0.7
pH pH 8 N.D. -1.2 -2.4 -0.1 +0.0
pH Growth at pH6 and (C) Trisodium citrate N.D. -2.6 -1.1 +0.2 -0.1
carbon source Valeric (C) N.D. -0.4 -0.5 -0.8 -1.9
pH pH 6 N.D. -1.5 -1.3 -0.0 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -1.4 -1.3 +0.6 -1.1
temperature shift Temperature shift 10_to_34; with MOPS N.D. -1.5 -0.9 -0.6 -0.0
nitrogen source nitrate (N) N.D. -0.0 -2.2 +0.1 -0.9
pH Growth at pH9 and (C) D-Glucose N.D. -1.2 -1.6 -0.4 +0.2
pH Growth at pH8 and (C) D-Glucose N.D. -1.4 -1.5 +0.0 -0.1
nitrogen source Urea (N) N.D. -1.5 +0.1 -0.1 -1.5
nitrogen source Ammonium chloride (N) N.D. -0.2 -1.7 -0.1 -0.8
carbon source carbon source D-Glucose 55.5 mM N.D. -1.6 -1.7 +0.9 -0.1
temperature Growth at 30C; with MOPS N.D. +0.6 -1.7 -0.8 -0.6
temperature shift Temperature shift 10_to_30; with MOPS N.D. -0.7 -2.1 +0.2 +0.2
carbon source carbon source Bisabolene 48.9 mM N.D. -1.5 -0.9 +0.4 -0.3
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -1.5 -0.4 +0.3 -0.7
temperature Growth at 25C N.D. -1.0 -1.6 +0.0 +0.6
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. -1.6 -0.6 +0.7 -0.1
carbon source 4-Aminobutyric 10 mM (C) N.D. +0.5 -1.8 +0.2 -0.6
temperature shift Temperature shift 10_to_25; with MOPS N.D. -1.0 -1.1 -0.3 +0.8
pH pH 9 N.D. -1.5 -0.3 +0.9 -0.4
temperature Growth at 25C N.D. -1.2 +0.1 -0.7 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 -0.2 +0.4 -1.6
phage PUT 8 MOI 1 N.D. +1.4 -1.3 -0.5 +0.2
nitrogen source nitrogen source nitrate 5mM N.D. +0.6 -1.5 +0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 +0.4 -0.4 +1.6
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