Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3021 and PP_3022 are separated by 81 nucleotidesPP_3022 and PP_3024 are separated by 535 nucleotidesPP_3024 and PP_5550 overlap by 14 nucleotidesPP_5550 and PP_5551 are separated by 13 nucleotides PP_3021: PP_3021 - Transporter, LysE family, at 3,407,929 to 3,408,543 _3021 PP_3022: PP_3022 - Transcriptional regulator, AraC family, at 3,408,625 to 3,409,434 _3022 PP_3024: PP_3024 - conserved protein of unknown function, at 3,409,970 to 3,411,166 _3024 PP_5550: PP_5550 - conserved protein of unknown function, at 3,411,153 to 3,411,473 _5550 PP_5551: PP_5551 - conserved protein of unknown function, at 3,411,487 to 3,412,011 _5551
Group Condition PP_3021 PP_3022 PP_3024 PP_5550 PP_5551
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.6 -0.1 -0.5 N.D. -0.3
nitrogen source D-2-Aminobutyric (N) -0.9 -0.5 -0.1 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 +0.5 -1.1 N.D. +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.1 +0.1 -0.5 N.D. -0.8
carbon source Tween 20 (C) -0.8 -0.3 +0.3 N.D. -0.3
pH Growth at pH6 and (C) Trisodium citrate -1.0 +0.1 +0.2 N.D. -0.2
stress Growth with Chloride 500 mM; with MOPS -0.5 -0.4 +0.5 N.D. -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.4 -0.3 -0.3 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.2 +0.3 N.D. -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.3 -0.2 -0.8 N.D. +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.0 -0.6 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.3 -0.6 N.D. +0.2
phage P. putida Sci small MOI 0.1 -0.8 -0.2 -0.1 N.D. +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +1.3 -0.5 N.D. -0.6
pH Growth at pH6 and (C) Trisodium citrate -0.3 +0.1 -0.6 N.D. +0.6
pH Growth at pH9 and (C) Trisodium citrate -0.2 -0.3 +0.7 N.D. -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.2 +0.1 -0.9 N.D. +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.2 -0.5 N.D. +0.6
pH Growth at pH7 and (C) Trisodium citrate -0.8 +0.1 +0.2 N.D. +0.6
phage P. putida PUT 7 MOI 1 +0.1 -0.8 +0.4 N.D. +0.4
phage P. putida Sci Big MOI 0.1 +0.4 +0.3 +0.5 N.D. -0.8
nitrogen source DL-2-Aminobutyric (N) -0.2 +0.0 +0.6 N.D. +0.6
phage PUT8 MOI 0.1 -0.2 -0.3 +0.7 N.D. +0.8
phage PUT 8 MOI 1 -0.1 -0.2 +0.6 N.D. +0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.9 -0.3 N.D. +0.5
phage PUT8 MOI 10 +0.1 +0.2 +0.4 N.D. +0.9
phage PUT8 MOI 10 +0.0 -0.2 +0.8 N.D. +1.0
phage P. putida Missley MOI 0.1 +0.7 +0.3 +0.5 N.D. +0.1
stress 1-Pentanol 0.3 vol% +0.5 +0.6 +2.2 N.D. +2.1
stress 3-methyl-1-butanol 0.4 vol% +0.6 +0.6 +2.6 N.D. +2.8
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