Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2992 and PP_2994 are separated by 299 nucleotidesPP_2994 and PP_2995 are separated by 167 nucleotidesPP_2995 and PP_2996 are separated by 62 nucleotidesPP_2996 and PP_2997 are separated by 228 nucleotides PP_2992: PP_2992 - conserved exported protein of unknown function, at 3,389,688 to 3,390,491 _2992 PP_2994: PP_2994 - Oxidoreductase, FMN-binding, at 3,390,791 to 3,392,047 _2994 PP_2995: PP_2995 - putative DNA topology modulation kinase FlaR, at 3,392,215 to 3,392,757 _2995 PP_2996: PP_2996 - conserved protein of unknown function, at 3,392,820 to 3,393,278 _2996 PP_2997: PP_2997 - CnmA, at 3,393,507 to 3,394,406 _2997
Group Condition PP_2992 PP_2994 PP_2995 PP_2996 PP_2997
carbon source carbon source 4-vinylphenol 0.01 mM -0.3 -0.2 -0.7 -0.4 -0.5
temperature Growth at 25C; with MOPS -0.3 -0.3 -0.2 +0.0 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.2 -1.5 +0.0 -0.5
carbon source carbon source 3-O-methyl-glucose 50 mM -0.5 +0.4 -1.1 -0.4 -0.2
carbon source Crotonic (C) -0.1 -0.4 -0.4 -0.4 -0.6
carbon source Lauric (C) -0.5 -0.4 -0.4 -0.2 -0.2
stress 1-Pentanol 0.3 vol% -0.5 -0.2 -0.5 -0.3 -0.1
pH Growth at pH6 and (C) Trisodium citrate -0.1 +0.1 -0.4 -0.3 -0.8
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -0.4 +0.0 -0.4 -0.5 -0.2
carbon source carbon source 3-O-methyl-glucose 10 mM +0.1 +0.1 -0.4 -0.6 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.3 +0.3 -0.0 -0.7
phage P. putida Sci small MOI 0.1 -0.5 -0.6 -0.0 +0.4 -0.2
carbon source L-Leucine (C) -0.5 +0.2 +0.2 -0.1 -0.6
carbon source ferulate (C); with MOPS -0.6 +0.2 -0.3 +0.4 -0.5
carbon source ferulate (C); with MOPS -0.6 +0.1 +0.2 -0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.2 -0.3 +0.1 -0.8
nitrogen source NAG (N); with MOPS -0.5 +0.4 +0.1 -0.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.3 -0.5 +0.3 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.3 -0.4 +0.3 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.5 +0.0 -0.2 +0.3 -0.6
nitrogen source Urea (N); with MOPS -0.6 +0.5 +0.3 -0.0 -0.2
carbon source 4-Aminobutyric 10 mM (C) +0.3 -0.0 -0.5 +0.5 -0.2
nitrogen source D-2-Aminobutyric (N) +0.9 -0.1 -0.2 +0.2 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.4 -0.2 +0.2 +0.5 +0.2
phage P. putida Sci Big MOI 0.1 +0.6 +0.6 -1.0 +0.3 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.4 -0.7 +0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 +0.4 +0.3 -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 -0.3 +0.4 +0.2 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.4 +0.6 -0.3 +0.4 -0.0
nitrogen source 2,6-diaminopimelic (N) +0.2 +0.7 +0.3 -0.2 +0.3
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