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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5548 and PP_2986 are separated by 78 nucleotides
PP_2986 and PP_2987 are separated by 200 nucleotides
PP_2987 and PP_2988 are separated by 96 nucleotides
PP_2988 and PP_2989 are separated by 46 nucleotides
PP_5548: PP_5548 - protein of unknown function, at 3,384,184 to 3,384,288
_5548
PP_2986: PP_2986 - putative Oxidoreductase, at 3,384,367 to 3,385,416
_2986
PP_2987: PP_2987 - conserved protein of unknown function, at 3,385,617 to 3,386,060
_2987
PP_2988: PP_2988 - Alcohol dehydrogenase, zinc-containing, at 3,386,157 to 3,387,173
_2988
PP_2989: PP_2989 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,387,220 to 3,388,014
_2989
Group
Condition
PP
_5548
PP
_2986
PP
_2987
PP
_2988
PP
_2989
phage
P. putida South MOI 10
-2.5
+0.2
-0.0
-0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
-0.3
-0.8
-0.4
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.1
+0.2
-0.7
-0.2
-0.2
nitrogen source
NAG (N); with MOPS
-1.5
+0.2
-0.9
+0.1
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.4
+0.0
-1.0
-0.2
-0.4
carbon source
carbon source D-Glucose 55.5 mM
-2.1
-0.3
+0.4
+0.1
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-1.3
+0.3
+0.1
-0.4
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-1.6
-0.1
+0.0
+0.3
-0.2
pH
Growth at pH9 and (C) Trisodium citrate
-0.8
+0.1
+0.1
-0.8
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.8
-0.2
+0.5
+0.2
-0.2
nitrogen source
Hippuric (N)
-0.8
+0.2
+0.2
-0.3
-0.5
pH
Growth at pH7 and (C) Trisodium citrate
+0.7
-0.2
-0.8
-0.8
-0.0
phage
P. putida Sci small MOI 0.1
-1.4
-0.1
+0.4
+0.1
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
+0.4
-0.5
-0.0
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.4
+0.1
+0.5
-0.3
-0.5
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.2
-0.8
-0.2
+0.5
pH
pH 6
+0.3
-0.2
+0.7
-0.2
-1.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
+0.2
-0.6
+0.1
-0.4
temperature
Growth at 30C; with MOPS
+1.0
+0.2
-0.3
-0.1
-0.3
phage
P. putida Sci Big MOI 0.1
-0.5
+0.7
+1.0
-0.8
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.0
+0.1
-0.7
-0.2
+0.5
phage
P. putida PUT 7 MOI 1
+0.8
+0.3
+0.5
-0.5
-0.3
carbon source
3-methyl-1-butanol 0.25 vol% (C)
+1.1
+0.5
-0.5
+0.1
-0.2
r2a control
R2A control
+1.3
+0.1
-0.5
+0.0
+0.1
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
+0.9
+0.0
-0.7
+0.3
+0.6
phage
PUT 8 MOI 1
+1.1
+0.2
+0.4
-0.2
-0.1
pH
pH 9
+0.8
-0.2
+1.2
-0.1
-0.4
carbon source
ferulate (C); with MOPS
+1.2
+0.1
+0.4
+0.0
-0.2
phage
P. putida Sci small MOI 0.1
+1.5
+0.2
+0.3
-0.2
-0.0
phage
P. putida Missley MOI 0.1
+1.8
+0.4
+0.4
+0.2
-0.2
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