Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5548 and PP_2986 are separated by 78 nucleotidesPP_2986 and PP_2987 are separated by 200 nucleotidesPP_2987 and PP_2988 are separated by 96 nucleotidesPP_2988 and PP_2989 are separated by 46 nucleotides PP_5548: PP_5548 - protein of unknown function, at 3,384,184 to 3,384,288 _5548 PP_2986: PP_2986 - putative Oxidoreductase, at 3,384,367 to 3,385,416 _2986 PP_2987: PP_2987 - conserved protein of unknown function, at 3,385,617 to 3,386,060 _2987 PP_2988: PP_2988 - Alcohol dehydrogenase, zinc-containing, at 3,386,157 to 3,387,173 _2988 PP_2989: PP_2989 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,387,220 to 3,388,014 _2989
Group Condition PP_5548 PP_2986 PP_2987 PP_2988 PP_2989
phage P. putida South MOI 10 -2.5 +0.2 -0.0 -0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.3 -0.8 -0.4 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.1 +0.2 -0.7 -0.2 -0.2
nitrogen source NAG (N); with MOPS -1.5 +0.2 -0.9 +0.1 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.4 +0.0 -1.0 -0.2 -0.4
carbon source carbon source D-Glucose 55.5 mM -2.1 -0.3 +0.4 +0.1 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -1.3 +0.3 +0.1 -0.4 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -1.6 -0.1 +0.0 +0.3 -0.2
pH Growth at pH9 and (C) Trisodium citrate -0.8 +0.1 +0.1 -0.8 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.8 -0.2 +0.5 +0.2 -0.2
nitrogen source Hippuric (N) -0.8 +0.2 +0.2 -0.3 -0.5
pH Growth at pH7 and (C) Trisodium citrate +0.7 -0.2 -0.8 -0.8 -0.0
phage P. putida Sci small MOI 0.1 -1.4 -0.1 +0.4 +0.1 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 +0.4 -0.5 -0.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 +0.1 +0.5 -0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.2 -0.8 -0.2 +0.5
pH pH 6 +0.3 -0.2 +0.7 -0.2 -1.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.2 -0.6 +0.1 -0.4
temperature Growth at 30C; with MOPS +1.0 +0.2 -0.3 -0.1 -0.3
phage P. putida Sci Big MOI 0.1 -0.5 +0.7 +1.0 -0.8 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 +0.1 -0.7 -0.2 +0.5
phage P. putida PUT 7 MOI 1 +0.8 +0.3 +0.5 -0.5 -0.3
carbon source 3-methyl-1-butanol 0.25 vol% (C) +1.1 +0.5 -0.5 +0.1 -0.2
r2a control R2A control +1.3 +0.1 -0.5 +0.0 +0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.9 +0.0 -0.7 +0.3 +0.6
phage PUT 8 MOI 1 +1.1 +0.2 +0.4 -0.2 -0.1
pH pH 9 +0.8 -0.2 +1.2 -0.1 -0.4
carbon source ferulate (C); with MOPS +1.2 +0.1 +0.4 +0.0 -0.2
phage P. putida Sci small MOI 0.1 +1.5 +0.2 +0.3 -0.2 -0.0
phage P. putida Missley MOI 0.1 +1.8 +0.4 +0.4 +0.2 -0.2
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