Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2972 and PP_2973 are separated by 38 nucleotidesPP_2973 and PP_2974 are separated by 34 nucleotidesPP_2974 and PP_2975 are separated by 65 nucleotidesPP_2975 and PP_2976 are separated by 147 nucleotides PP_2972: PP_2972 - conserved protein of unknown function, at 3,373,715 to 3,374,014 _2972 PP_2973: PP_2973 - diacylglycerol kinase, at 3,374,053 to 3,374,406 _2973 PP_2974: PP_2974 - putative sulfatase, at 3,374,441 to 3,376,093 _2974 PP_2975: PP_2975 - Insertion element ISR1 uncharacterized 10 kDa protein A3, at 3,376,159 to 3,376,425 _2975 PP_2976: PP_2976 - putative transposase, at 3,376,573 to 3,376,884 _2976
Group Condition PP_2972 PP_2973 PP_2974 PP_2975 PP_2976
phage PUT 8 MOI 1 -6.8 +0.2 +0.4 -0.3 +0.1
pH Growth at pH7 and (C) Trisodium citrate -0.6 +0.1 -0.1 -0.3 -4.3
pH Growth at pH7 and (C) D-Glucose -6.6 -0.1 -0.0 +0.2 +1.3
temperature Growth at 30C; with MOPS -0.7 -0.0 +0.1 +0.1 -4.3
temperature shift Temperature shift 10_to_25; with MOPS -0.0 +0.0 -0.3 +0.1 -4.2
carbon source ferulate (C); with MOPS -0.6 +0.1 -0.1 +0.3 -4.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.4 +0.1 -0.0 -3.3
nitrogen source nitrate (N) +0.1 -0.2 -0.0 +0.4 -3.4
stress Butyrolactam 2 vol% -0.7 -0.2 -0.2 -0.3 -1.7
pH Growth at pH7 and (C) Trisodium citrate -3.9 +0.5 +0.2 +0.3 +0.1
temperature shift Temperature shift 30_to_21; with MOPS -1.2 +0.3 -0.2 -1.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 +0.2 +0.0 +0.2 -2.2
nitrogen source Ammonium chloride and TNT (N) +0.6 -0.4 -0.3 -0.1 -2.0
pH Growth at pH8 and (C) Trisodium citrate +0.5 -0.2 -0.1 +0.0 -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.2 -0.2 -0.2 +0.2 +0.5
stress R2A with Fusaric 240 ug/mL -0.9 +0.2 +0.3 +0.2 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -2.0 -0.3 +0.3 +0.2 +0.7
r2a control with 0.2% methanol R2A control with 0.2% methanol -1.6 -0.5 +0.3 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.8 -0.4 -0.1 -0.1 +1.3
phage P. putida Sci Big MOI 0.1 +0.8 +0.6 -0.1 +0.4 -2.6
nitrogen source no extra (N); with MOPS -1.4 -0.6 -0.1 +0.1 +1.4
temperature shift Temperature shift 30_to_34; with MOPS -1.4 -0.4 +0.0 +0.2 +0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.0 +0.3 +0.1 -0.9 -0.7
temperature shift Temperature shift 30_to_25; with MOPS -1.4 -0.1 -0.0 +0.4 +1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +1.4 +0.1 +0.0 +0.3 -1.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 -0.4 -0.1 +0.1 +2.2
pH pH 9 +0.8 -0.4 +0.1 +0.4 +1.8
carbon source carbon source 3-O-methyl-glucose 50 mM +1.4 -0.3 -0.1 +0.4 +1.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.5 +0.1 +0.0 +0.2 +0.4
pH pH 6 +1.4 -0.3 +0.0 +0.4 +1.9
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