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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_2972 and PP_2973 are separated by 38 nucleotides
PP_2973 and PP_2974 are separated by 34 nucleotides
PP_2974 and PP_2975 are separated by 65 nucleotides
PP_2975 and PP_2976 are separated by 147 nucleotides
PP_2972: PP_2972 - conserved protein of unknown function, at 3,373,715 to 3,374,014
_2972
PP_2973: PP_2973 - diacylglycerol kinase, at 3,374,053 to 3,374,406
_2973
PP_2974: PP_2974 - putative sulfatase, at 3,374,441 to 3,376,093
_2974
PP_2975: PP_2975 - Insertion element ISR1 uncharacterized 10 kDa protein A3, at 3,376,159 to 3,376,425
_2975
PP_2976: PP_2976 - putative transposase, at 3,376,573 to 3,376,884
_2976
Group
Condition
PP
_2972
PP
_2973
PP
_2974
PP
_2975
PP
_2976
phage
PUT 8 MOI 1
-6.8
+0.2
+0.4
-0.3
+0.1
pH
Growth at pH7 and (C) Trisodium citrate
-0.6
+0.1
-0.1
-0.3
-4.3
pH
Growth at pH7 and (C) D-Glucose
-6.6
-0.1
-0.0
+0.2
+1.3
temperature
Growth at 30C; with MOPS
-0.7
-0.0
+0.1
+0.1
-4.3
temperature shift
Temperature shift 10_to_25; with MOPS
-0.0
+0.0
-0.3
+0.1
-4.2
carbon source
ferulate (C); with MOPS
-0.6
+0.1
-0.1
+0.3
-4.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
-0.4
+0.1
-0.0
-3.3
nitrogen source
nitrate (N)
+0.1
-0.2
-0.0
+0.4
-3.4
stress
Butyrolactam 2 vol%
-0.7
-0.2
-0.2
-0.3
-1.7
pH
Growth at pH7 and (C) Trisodium citrate
-3.9
+0.5
+0.2
+0.3
+0.1
temperature shift
Temperature shift 30_to_21; with MOPS
-1.2
+0.3
-0.2
-1.1
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.6
+0.2
+0.0
+0.2
-2.2
nitrogen source
Ammonium chloride and TNT (N)
+0.6
-0.4
-0.3
-0.1
-2.0
pH
Growth at pH8 and (C) Trisodium citrate
+0.5
-0.2
-0.1
+0.0
-2.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-2.2
-0.2
-0.2
+0.2
+0.5
stress
R2A with Fusaric 240 ug/mL
-0.9
+0.2
+0.3
+0.2
-1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-2.0
-0.3
+0.3
+0.2
+0.7
r2a control with 0.2% methanol
R2A control with 0.2% methanol
-1.6
-0.5
+0.3
+0.2
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.8
-0.4
-0.1
-0.1
+1.3
phage
P. putida Sci Big MOI 0.1
+0.8
+0.6
-0.1
+0.4
-2.6
nitrogen source
no extra (N); with MOPS
-1.4
-0.6
-0.1
+0.1
+1.4
temperature shift
Temperature shift 30_to_34; with MOPS
-1.4
-0.4
+0.0
+0.2
+0.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+1.0
+0.3
+0.1
-0.9
-0.7
temperature shift
Temperature shift 30_to_25; with MOPS
-1.4
-0.1
-0.0
+0.4
+1.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+1.4
+0.1
+0.0
+0.3
-1.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.8
-0.4
-0.1
+0.1
+2.2
pH
pH 9
+0.8
-0.4
+0.1
+0.4
+1.8
carbon source
carbon source 3-O-methyl-glucose 50 mM
+1.4
-0.3
-0.1
+0.4
+1.2
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.5
+0.1
+0.0
+0.2
+0.4
pH
pH 6
+1.4
-0.3
+0.0
+0.4
+1.9
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