Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2969 and PP_2970 are separated by 118 nucleotidesPP_2970 and PP_2971 are separated by 291 nucleotidesPP_2971 and PP_2972 overlap by 165 nucleotidesPP_2972 and PP_2973 are separated by 38 nucleotides PP_2969: PP_2969 - putative repressor of copper utilisation proteins, at 3,371,785 to 3,372,072 _2969 PP_2970: PP_2970 - conserved exported protein of unknown function, at 3,372,191 to 3,372,550 _2970 PP_2971: PP_2971 - transposase, at 3,372,842 to 3,373,879 _2971 PP_2972: PP_2972 - conserved protein of unknown function, at 3,373,715 to 3,374,014 _2972 PP_2973: PP_2973 - diacylglycerol kinase, at 3,374,053 to 3,374,406 _2973
Group Condition PP_2969 PP_2970 PP_2971 PP_2972 PP_2973
pH Growth at pH7 and (C) D-Glucose -0.5 -0.7 -0.1 -6.6 -0.1
phage PUT 8 MOI 1 -0.1 +0.6 +0.2 -6.8 +0.2
pH Growth at pH7 and (C) Trisodium citrate -0.2 +0.0 -0.4 -3.9 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -2.1 +0.1 -2.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 -1.3 +0.4 -1.8 -0.4
nitrogen source no extra (N); with MOPS +0.3 -1.1 -0.1 -1.4 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.1 -0.3 -2.3 +0.1
carbon source D-Glucose (C) with 20AA_mix_minus_Cys +0.1 -1.6 -0.2 -0.7 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 +1.0 -0.5 -2.2 -0.2
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.2 +0.5 +0.2 -1.6 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.2 +0.9 +0.0 +0.1 -0.7
no stress control Growth with no stress -0.6 +0.3 +0.6 -1.2 +0.1
carbon source D-Glucose (C) -0.3 -1.8 +0.0 +1.0 +0.4
temperature shift Temperature shift 30_to_34; with MOPS +0.1 +0.8 +0.2 -1.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -1.3 -0.1 +0.9 -0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.1 +0.9 +0.3 -1.4 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.0 +1.1 -1.0 -0.7 +0.0
temperature shift Temperature shift 30_to_25; with MOPS -0.2 -1.2 +0.2 +1.4 -0.1
pH pH 9 -0.8 +0.7 +0.0 +0.8 -0.4
phage P. putida PUT 7 MOI 1 +0.6 -1.7 +0.2 +1.2 +0.0
phage P. putida Sci small MOI 0.1 -1.5 -0.3 +0.6 +1.2 +0.4
pH Growth at pH6 and (C) D-Glucose -0.8 +0.6 +0.4 +0.8 -0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.1 +1.5 -0.0 -1.1 +0.3
phage P. putida Sci Big MOI 0.1 +0.1 -1.5 +0.6 +0.8 +0.6
carbon source carbon source D-Glucose 55.5 mM -0.6 -0.4 +0.5 +1.6 -0.4
stress R2A with Fusaric 240 ug/mL +0.4 +1.3 -0.3 -0.9 +0.2
pH pH 6 -0.7 +0.7 -0.0 +1.4 -0.3
nitrogen source nitrogen source nitrite 0.5mM +0.4 +1.1 +0.2 +1.0 -0.0
nitrogen source 2,6-diaminopimelic (N) +0.5 +0.9 +0.7 +0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.8 +0.6 +2.5 +0.1
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