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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2969 and PP_2970 are separated by 118 nucleotides
PP_2970 and PP_2971 are separated by 291 nucleotides
PP_2971 and PP_2972 overlap by 165 nucleotides
PP_2972 and PP_2973 are separated by 38 nucleotides
PP_2969: PP_2969 - putative repressor of copper utilisation proteins, at 3,371,785 to 3,372,072
_2969
PP_2970: PP_2970 - conserved exported protein of unknown function, at 3,372,191 to 3,372,550
_2970
PP_2971: PP_2971 - transposase, at 3,372,842 to 3,373,879
_2971
PP_2972: PP_2972 - conserved protein of unknown function, at 3,373,715 to 3,374,014
_2972
PP_2973: PP_2973 - diacylglycerol kinase, at 3,374,053 to 3,374,406
_2973
Group
Condition
PP
_2969
PP
_2970
PP
_2971
PP
_2972
PP
_2973
pH
Growth at pH7 and (C) D-Glucose
-0.5
-0.7
-0.1
-6.6
-0.1
phage
PUT 8 MOI 1
-0.1
+0.6
+0.2
-6.8
+0.2
pH
Growth at pH7 and (C) Trisodium citrate
-0.2
+0.0
-0.4
-3.9
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.3
-2.1
+0.1
-2.0
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.3
-1.3
+0.4
-1.8
-0.4
nitrogen source
no extra (N); with MOPS
+0.3
-1.1
-0.1
-1.4
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
-0.1
-0.3
-2.3
+0.1
carbon source
D-Glucose (C) with 20AA_mix_minus_Cys
+0.1
-1.6
-0.2
-0.7
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.2
+1.0
-0.5
-2.2
-0.2
r2a control with 0.2% methanol
R2A control with 0.2% methanol
+0.2
+0.5
+0.2
-1.6
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.2
+0.9
+0.0
+0.1
-0.7
no stress control
Growth with no stress
-0.6
+0.3
+0.6
-1.2
+0.1
carbon source
D-Glucose (C)
-0.3
-1.8
+0.0
+1.0
+0.4
temperature shift
Temperature shift 30_to_34; with MOPS
+0.1
+0.8
+0.2
-1.4
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.1
-1.3
-0.1
+0.9
-0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.1
+0.9
+0.3
-1.4
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.0
+1.1
-1.0
-0.7
+0.0
temperature shift
Temperature shift 30_to_25; with MOPS
-0.2
-1.2
+0.2
+1.4
-0.1
pH
pH 9
-0.8
+0.7
+0.0
+0.8
-0.4
phage
P. putida PUT 7 MOI 1
+0.6
-1.7
+0.2
+1.2
+0.0
phage
P. putida Sci small MOI 0.1
-1.5
-0.3
+0.6
+1.2
+0.4
pH
Growth at pH6 and (C) D-Glucose
-0.8
+0.6
+0.4
+0.8
-0.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.1
+1.5
-0.0
-1.1
+0.3
phage
P. putida Sci Big MOI 0.1
+0.1
-1.5
+0.6
+0.8
+0.6
carbon source
carbon source D-Glucose 55.5 mM
-0.6
-0.4
+0.5
+1.6
-0.4
stress
R2A with Fusaric 240 ug/mL
+0.4
+1.3
-0.3
-0.9
+0.2
pH
pH 6
-0.7
+0.7
-0.0
+1.4
-0.3
nitrogen source
nitrogen source nitrite 0.5mM
+0.4
+1.1
+0.2
+1.0
-0.0
nitrogen source
2,6-diaminopimelic (N)
+0.5
+0.9
+0.7
+0.4
+0.8
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.8
+0.6
+2.5
+0.1
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