Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2927 and PP_2928 are separated by 130 nucleotidesPP_2928 and PP_2929 are separated by 32 nucleotidesPP_2929 and PP_2930 are separated by 185 nucleotidesPP_2930 and PP_2931 overlap by 4 nucleotides PP_2927: PP_2927 - conserved protein of unknown function, at 3,328,450 to 3,329,496 _2927 PP_2928: PP_2928 - Saccharopine dehydrogenase, at 3,329,627 to 3,330,871 _2928 PP_2929: PP_2929 - Carboxynorspermidine/carboxyspermidine decarboxylase, at 3,330,904 to 3,332,001 _2929 PP_2930: PP_2930 - putative L-serine dehydratase, at 3,332,187 to 3,333,104 _2930 PP_2931: PP_2931 - conserved protein of unknown function, at 3,333,101 to 3,333,805 _2931
Group Condition PP_2927 PP_2928 PP_2929 PP_2930 PP_2931
pH Growth at pH7 and (C) D-Glucose -0.1 -1.5 -0.9 -0.6 -0.7
pH Growth at pH6 and (C) D-Glucose -0.4 -0.3 -0.5 -1.0 -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.9 -1.8 -0.2 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 -0.1 -0.3 -2.8 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.5 -1.2 -1.4 -0.3 -0.3
carbon source D-Glucose (C) with 20AA_mix_minus_Arg -0.3 -1.4 -1.5 -0.0 -0.4
carbon source Sorghum Hydrolysate (C) -0.2 -1.3 -1.6 -0.3 -0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.2 -1.6 -2.0 +0.4 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.7 -1.5 +0.1 -0.5
carbon source D-Glucose (C) with 20AA_mix_minus_Gly -0.5 -1.6 -1.1 +0.2 -0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.2 -1.7 -1.6 +0.3 +0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.2 -1.7 -1.5 +0.3 -0.0
stress R2A with Fusaric 480 ug/mL -0.3 -1.0 -1.6 -0.3 +0.2
stress Growth with Chloride 200 mM; with MOPS -0.2 -1.7 -1.0 -0.4 +0.3
pH Growth at pH8 and (C) D-Glucose +0.4 -1.1 -1.3 -0.1 -0.7
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -1.6 -1.7 -0.1 +0.8
nitrogen source Propandiamine (N) +0.3 -1.4 -2.2 +0.2 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.0 -1.1 -1.2 -1.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.7 -1.2 +0.4 -1.4
stress R2A with Fusaric 480 ug/mL +0.2 -1.3 -1.1 -0.6 +0.4
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL +0.6 -1.2 -1.3 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.7 -1.0 -1.1 +0.5 +0.2
nitrogen source Ammonium chloride (N) +0.4 -1.0 -1.2 +0.0 +0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 -1.2 -2.1 +1.1 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -1.2 -0.9 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 -0.7 -1.2 -0.1 +0.4
phage P. putida South MOI 0.1 -0.3 +0.9 +1.4 -0.1 -1.6
nitrogen source DL-2-Aminobutyric (N) +0.0 +1.4 +2.0 -0.0 -0.4
phage P. putida South MOI 1 +0.2 +1.0 +1.7 +0.0 +0.5
nitrogen source DL-2-Aminobutyric (N) +0.4 +1.4 +2.1 -0.0 -0.1
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