Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2922 and PP_2923 are separated by 62 nucleotidesPP_2923 and PP_2924 overlap by 10 nucleotidesPP_2924 and PP_2925 are separated by 114 nucleotidesPP_2925 and PP_2926 are separated by 9 nucleotides PP_2922: PP_2922 - Urea amidolyase-related protein, at 3,322,001 to 3,322,972 _2922 PP_2923: PP_2923 - conserved protein of unknown function, at 3,323,035 to 3,323,892 _2923 PP_2924: PP_2924 - conserved protein of unknown function, at 3,323,883 to 3,325,133 _2924 PP_2925: PP_2925 - malate:quinone oxidoreductase, at 3,325,248 to 3,326,867 _2925 PP_2926: PP_2926 - UDP-glucose 6-dehydrogenase, at 3,326,877 to 3,328,205 _2926
Group Condition PP_2922 PP_2923 PP_2924 PP_2925 PP_2926
nitrogen source Butyrolactam (N) -5.1 +0.6 -0.0 -0.1 +0.1
nitrogen source Butyrolactam (N) -3.8 +0.1 -0.0 -0.0 +0.1
carbon source Trisodium citrate (C) -1.1 +0.2 -0.3 -0.5 -0.1
stress R2A with Fusaric 240 ug/mL +0.1 -1.3 -0.2 -0.6 +0.2
nitrogen source NAG (N); with MOPS -1.8 -0.3 +0.1 +0.3 +0.2
temperature Growth at 30C; with MOPS -1.6 +0.2 -0.1 +0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 -0.4 +0.0 -0.1 +0.5
nitrogen source Propiolactam (N) -2.3 +0.7 +0.4 +0.0 -0.1
carbon source 1,3-Butandiol (C) +0.2 -1.5 -0.2 +0.3 -0.1
nitrogen source nitrite (N) -1.0 +0.3 +0.2 -0.7 -0.2
carbon source D-Glucose (C) -0.4 -1.7 +0.4 -0.2 +0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -1.6 +0.0 -0.0 +0.2 +0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.6 +0.3 -0.5 -0.6
temperature shift Temperature shift 30_to_21; with MOPS -1.6 +0.4 +0.2 +0.1 +0.1
nitrogen source Urea (N) -1.8 +0.6 -0.1 +0.1 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.7 +0.3 +0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -0.4 -0.3 -0.5 +0.7
pH Growth at pH6 and (C) Trisodium citrate -0.6 +0.5 -0.1 +0.1 -0.5
temperature shift Temperature shift 10_to_25; with MOPS -1.1 +0.5 -0.1 +0.2 -0.0
no stress control Growth with no stress -1.2 -0.0 -0.1 +0.0 +0.9
stress Growth with Chloride 500 mM; with MOPS -0.7 +0.8 -0.2 -0.2 +0.1
nitrogen source Propiolactam (N) -1.6 +1.1 +0.4 +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +1.1 -0.0 -0.4 -0.1
carbon source carbon source D-Glucose 55.5 mM +0.3 +0.6 +0.2 -1.0 +0.2
temperature Growth at 25C -0.8 -0.0 +0.1 +0.5 +0.9
pH pH 8 -0.0 +1.2 +0.3 -0.7 +0.1
phage P. putida Sci small MOI 0.1 +0.3 +1.6 -0.2 -0.1 -0.6
carbon source Myristic (C) +0.8 +0.7 -0.4 -0.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.7 -0.2 +0.7 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.5 +0.3 +0.3 +0.7 +0.2
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