Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2916 and PP_2918 are separated by 172 nucleotidesPP_2918 and PP_2919 are separated by 191 nucleotidesPP_2919 and PP_2920 are separated by 120 nucleotidesPP_2920 and PP_2921 are separated by 38 nucleotides PP_2916: PP_2916 - riboflavin synthase, at 3,316,168 to 3,316,797 _2916 PP_2918: PP_2918 - trehalose synthase A, at 3,316,970 to 3,319,036 _2918 PP_2919: PP_2919 - Transcriptional regulator, LysR family, at 3,319,228 to 3,320,145 _2919 PP_2920: PP_2920 - putative nitrogen-containing compound degradation enzyme, at 3,320,266 to 3,321,024 _2920 PP_2921: PP_2921 - conserved protein of unknown function, at 3,321,063 to 3,322,004 _2921
Group Condition PP_2916 PP_2918 PP_2919 PP_2920 PP_2921
nitrogen source Butyrolactam (N) -0.0 +0.1 -4.6 -4.7 -4.4
nitrogen source Butyrolactam (N) -0.0 +0.0 -3.8 -4.3 -4.4
nitrogen source Propiolactam (N) +0.1 +0.6 -3.2 -4.4 -4.6
nitrogen source Propiolactam (N) +0.8 +0.5 -2.7 -3.1 -3.7
pH Valerolactone (C) pH 6.5 -0.9 -0.1 -0.1 -0.5 -0.4
nitrogen source 2,6-diaminopimelic (N) -0.9 -0.4 +0.5 -0.8 -0.2
carbon source carbon source 4-vinylphenol 0.01 mM -0.8 -0.2 -0.3 -0.2 -0.5
carbon source carbon source D-Glucose 55.5 mM -1.2 -0.5 +0.1 -0.0 -0.3
nitrogen source Imidazole (N) -0.8 -0.2 -0.4 +0.0 -0.4
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.5 -0.2 -0.6 -0.3 +0.3
phage P. putida South MOI 10 -0.5 -0.2 +0.5 -0.4 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.0 -0.4 +0.3 -1.2
carbon source Valerolactone (C) -0.3 -0.2 +0.3 -0.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 +0.2 +0.1 +0.3 -0.5
nitrogen source NAG (N); with MOPS -0.7 -0.2 +0.5 +0.4 -0.4
pH Growth at pH9 and (C) Trisodium citrate -0.9 -0.1 +0.3 +0.3 -0.1
pH pH 7 -0.9 -0.1 +0.1 +0.5 -0.1
pH Growth at pH7 and (C) Trisodium citrate -0.9 -0.4 -0.1 +0.5 +0.5
carbon source Acetate Carbon Source (20mM) +0.3 +0.2 +0.3 -0.4 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.0 -0.1 -0.6 +1.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.3 +0.5 -0.1 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 -0.0 -0.4 +0.5 -0.4
nitrogen source D-2-Aminobutyric (N) -0.3 +0.2 -0.3 +0.8 -0.1
phage P. putida Sci small MOI 0.1 -0.4 -0.1 -0.0 +0.8 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 +0.2 +0.1 +0.3 -0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.1 -0.5 +0.7 +1.2
pH Growth at pH6 and (C) D-Glucose +0.3 -0.3 +0.4 +0.5 -0.2
carbon source D-Glucose (C) -0.3 +0.2 +0.4 +0.7 -0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.4 +0.3 -0.7 +0.7 +0.6
pH Growth at pH7 and (C) D-Glucose +0.4 -0.2 +0.5 +0.6 +0.0
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